Two fatal intoxication cases with imidacloprid: LC/MS analysis

Imidacloprid [1-(6-chloro-3pyridylmethyl)-N-nitroimidazolidin-2-ylideneamine] is a new and potent nitromethylene insecticide with high insecticidal activity at very low application rates. It is the first highly effective insecticide that, like nicotine, acts on the nervous system, causing blockage of postsynaptic nicotinergic acetylcholine receptors. Two fatal cases with this insecticide in two male individuals, of 33 and 66 years old, are presented. An LC/MS with electrospray method for measuring imidacloprid and its metabolites in post-mortem samples is described. In the chromatographic separation, a reverse-phase column XTerra MS C18 (2.1mm i.d.x 150 mm, 5 microm) was used and the mobile phase composed with acetonitrile and 0.1% formic acid (15:85), at a 0.25 mL/min flow rate. Samples were prepared with a liquid-liquid extraction procedure with dichloromethane. Calibration curves for imidacloprid in blood and urine samples were linear from 0.2 to 15 microg/mL. The mean recovery was 86% with a coefficient of variation of +/-5.9%. The detection limit was 0.002 microg/mL. Quantitative results were obtained for all post-mortem matrices available of the two fatal cases: blood, urine, stomach contents, lung, liver and kidney. The imidacloprid blood concentrations found in two-cases were 12.5 and 2.05 microg/mL. The authors validated a method to detect and quantify imidacloprid in post-mortem samples, and to our knowledge for the first time a post-mortem tissue distribution was performed on various samples for this insecticide.     

A collaborative study of the EDNAP group regarding Y-chromosome binary polymorphism analysis

A collaborative study was carried out by the European DNA Profiling Group (EDNAP) in order to evaluate the performance of Y-chromosome binary polymorphism analysis in different European laboratories. Four blood samples were sent to the laboratories, to be analysed for 11 Y-chromosome single nucleotide polymorphisms (SNPs): SRY-1532, M40, M35, M213, M9, 92R7, M17, P25, M18, M153 and M167. All the labs were also asked to submit a population study including these markers. All participating laboratories reported the same results, indicating the reproducibility and robustness of Y-chromosome SNP typing. A total of 535 samples from six different European populations were also analysed. In Galicia (NW Spain) and Belgium, the most frequent haplogroup was R1b*(xR1b1,R1b3df). Haplogroup F*(xK) is one of the most frequent in Austria and Denmark, while the lowest frequency appear in Belgium. Haplogroup frequencies found in this collaborative study were compared with previously published European Y-chromosome haplogroup data.     

Political business cycles at the municipal level

The present article tests predictions of rational political business cycle models using a large and previously unexplored data set of Portuguese municipalities. This data allows for a clean test of these predictions due to the high level of detail on expenditure items, an exogenous fixed election schedule, and homogeneity of Portuguese local governments with respect to policy instruments and institutions. Estimation results clearly reveal the opportunistic behaviour of local governments. In pre-electoral periods, they increase total expenditures and change their composition favouring items that are highly visible to the electorate. This behaviour is consistent with an effort to signal competence and increase chances of re-election.     

Does It Take a Village? Policing Strategies and Fear of Crime in Latin America

How can policymakers reduce public fear of crime in Latin America? This study compares the effectiveness of “zero tolerance” and community‐based policing strategies in Argentina, Brazil, and Chile. At the micro level, it assesses the links between fear of crime and social identity characteristics, contextual factors, the media, community participation, and other insecurities. It finds that citizens' economic, political, and social insecurities are the main determinants of their fear of crime. At the macro level, the study compares levels of public insecurity and finds that cities that employ community‐based strategies to fight crime register lower levels of public fear of crime.     

Genetic profile of a multi-ethnic population from Guiné-Bissau (west African coast) using the new PowerPlex 16 System kit

Allele and haplotype frequencies of 15 chromosome STR loci included in the kit PowerPlex16 System from Promega, were determined in a sample of unrelated males from Guiné-Bissau, a country from the west African coast. All individuals were subjected to an interview in order to make sure that their ancestors belonged to the same ethnic group. This way we intended to look for possible inter-ethnic differences. PowerPlex 16 includes STRs not studied before in any multi-ethnic population. The kit includes two new allele markers (Penta D and Penta E), which are very useful either in forensics or population genetic studies. The Guinean population presents significant differences when compared with other African populations.     

Costing governmental services in a reformed environment: Unreachable goal or unfinished business?

This article assesses the impact of New Public Management (NPM) reforms in the public sector from a cost consciousness perspective. Using an action‐research methodology, we analyze the intensity of the external pressures that influence the institutional logic under which public sector organizations manage their costs. The field work shows that even after decentralization, public sector organizations appear unable to move their costing function from expense control to cost management unless external pressures motivate managers to complete the reform. We propose that the probability of success of reforms is increased when organizations are subject to strong pressures that could take the form of individual incentives or external threats.     

Les enjeux de l'évaluation de la performance : Dépasser les mythes

Since the 1990s, most Western countries have equipped themselves with frameworks and tools to measure health organizations and systems' performance. Paradoxically, several theoretical, methodological and usage issues remain unresolved. Conceptually, the notion of performance is vague and not well rooted in theory. From a methodological perspective, we are faced with numerous measures that don't always meet reliability, validity and usefulness criteria and don't help to grasp the complexity of the concept. Finally, the assessment results are not fully used to continuously improve performance. This article focuses on analyzing these issues by deconstructing the myths that slow down the development of better‐suited frameworks to meet decision‐makers' needs. It also proposes innovations based on the global and integrated assessment model of health systems.     

Implementing national food policies to promote local family agriculture: Belo Horizonte's story

Policies in Brazil promoting family agriculture such as the Food Acquisition Programme (PAA) and the 30% minimum procurement law for the National School Meals Programme have had varying success across the country. To understand why these policies work effectively only in some areas, the implementation processes must be clarified. This exploratory, ethnographic study identifies the causal mechanisms involved in implementing PAA and the 30% law in the city of Belo Horizonte. The findings reveal that although beneficial, large supply volumes and transportation costs are challenges faced by farmers in the implementation process.