Validation of the barcoding gene COI for use in forensic genetic species identification

The application of forensics to wildlife crime investigation routinely involves genetic species identification based on DNA sequence similarity. This work can be hindered by a lack of authenticated reference DNA sequence data resulting in weak matches between evidence and reference samples. The introduction of DNA barcoding has highlighted the expanding use of the mtDNA gene, cytochrome c oxidase I (COI), as a genetic marker for species identification. Here, we assess the COI gene for use in forensic analysis following published human validation guidelines. Validation experiments investigated reproducibility, heteroplasmy, mixed DNA, DNA template concentration, chemical treatments, substrate variation, environmental conditions and thermocycling parameters. Sequence similarity searches using both GenBank BLASTn and BOLD search engines indicated that the COI gene consistently identifies species where authenticated reference sequence data exists. Where misidentification occurred the cause was attributable to either erroneous reference sequences from published data, or lack of primer specificity. Although amplification failure was observed under certain sample treatments, there was no evidence of environmentally induced sequence mutation in those sequences that were generated. A simulated case study compared the performance of COI and cytochrome b mtDNA genes. Findings are discussed in relation to the utility of the COI gene in forensic species identification.     

Incapacitation: Revisiting an Old Question with a New Method and New Data

We use the National Longitudinal Survey of Youth 1997 to obtain estimates of the number of crimes avoided through incapacitation of individual offenders. Incarcerated individuals are matched to comparable non-incarcerated counterparts using propensity score matching. Propensity scores for incarceration are calculated using a wide variety of time-stable and time-varying confounding variables. We separately analyze juvenile (age 16 or 17) and adult (age 18 or 19) incapacitation effects. Our best estimate is that between 6.2 and 14.1 offenses are prevented per year of juvenile incarceration, and 4.9 to 8.4 offenses are prevented per year of adult incarceration.

Criminology Theory and Terrorism: Introduction to the Special Issue

In this short essay, we introduce readers to a special issue of Terrorism and Political Violence on criminological approaches to the study of terrorism. In addition to summarizing the eight articles in the issue, we outline some general points about the relationship between criminological thinking and our understanding of terrorism. Our goal is to place the special issue's contributions in context and highlight under-explored issues that future research could address.     

Proscribing torture: an analysis in Indian and ethical contexts

The article seeks context for the 2010 Indian Prevention of Torture Bill, by critically examining the nature of the international law proscribing torture, then the universality of torture’s immorality. Argument covers the scholarship on torture categories and addresses the probity of evidence deriving from tortured subjects. It critically investigates the sociological literature on torture. It states a suggested policy guide, developed in a worldwide context, and therefore of use by any jurisdiction. In particular, this paper considers the underpinnings of the prohibition against torture and also will analyse the proposed Indian Bill on the delimited basis of the conflicting ideologies of the two greater jurists, Immanuel Kant and Jeremy Bentham. This paper asks whether the new Prevention of Torture Bill, 2010 (India), still not enacted, would prevent torture in India.     

The Effects of a School Policing Program on Crime,Discipline, and Disorder: A Quasi-Experimental Evaluation

This study analyzes the impacts of school-based law enforcement officers on school crime, disciplinary actions, and disciplinary problems in 238 middle and high schools in West Virginia using a non-equivalent groups design and three years of data. Propensity score weights are utilized to reduce selection biases resulting from non-random group assignment in observational data. Binary and multinomial logistic treatment models are used when estimating treatment effects to examine whether the extent to which police officers are present in schools impacts problem outcomes. Findings indicated that while the presence of school police officers increased drug-related crimes and out-of-school suspensions for drug crimes regardless of whether they were present in schools for a single year or multiple years, there were deterrent effects observed for violent crimes and incidents of disorder when police officers were present in schools during all school years. Implications for policy and practice are discussed.

     

From intergenerational equity to intergenerational stewardship

This essay borrows from our civic humanist tradition to present an alternative to the more dominant neo-utilitarian approach to evaluating current decisions and their potential burden on future generations. The analysis first focuses on financial debt, and then applies the framework beyond the realm of financial policy. The framework exposes some limitations in the rational-quantitative approach to policy decision, and embraces the art of stewardship as a more encompassing and appropriate role in public life. Stewardship brings intergenerational obligations to bear on decisions that current analytic techniques fail to regard as meaningful. The ways and means of stewardship are matters more of prudent judgment and passionate commitment to ways of life than of rigorous analytic precision.

[A] man owes it to his children, to his neighbors to secure their future and rescue their lives from impediments to holiness and happiness. Therefore he has no right to acquiesce in tyrannical and immoral government.

Lord Acton⁽¹⁾