Use of bleach to eliminate contaminating DNA from the surface of bones and teeth

The extraction of DNA from archaeological or forensic skeletal remains can provide quite powerful data for analysis, but is plagued by a unique set of methodological problems. One of the most important methodological problems to overcome in such analyses is the presence of modern contamination on the surfaces of bones and teeth, which can lead to false positives and erroneous results unless it is removed before DNA extraction is initiated. Ancient DNA (aDNA) researchers and forensic scientists have employed a number of techniques to minimize such contamination. One such technique is the use of bleach (sodium hypochlorite--NaOCl) to "destroy" contaminating DNA. However, a consensus on the optimum concentration of sodium hypochlorite to be used and the amount of time the bone or tooth should be exposed to it has not emerged. The present study systematically approaches the issue by introducing contamination to ancient bones (from approximately 500 BP) and determining which of several sodium hypochlorite treatments best eliminates surface contamination. The elimination of surface contamination from bone requires immersion in at least 3.0% (w/v) sodium hypochlorite (approximately equal parts of commercial bleach and water) for at least 15 min. Endogenous DNA proved to be quite stable to even extreme sodium hypochlorite treatments (6% for 21 h), suggesting that DNA adsorbs to hydroxyapatite in the bone and that this process facilitates the preservation of DNA in ancient skeletal remains.     

Intra-country regulation of share markets: does one size fit all?

A large body of evidence suggests that financial development is greater in countries that impose stricter regulatory requirements on their major stock exchanges, but this leaves open the question of whether or not such regulation should be uniformly applied to all equity trading platforms within a country. On the one hand, regulatory variation permits a wider choice of investment opportunities for investors, lowers the cost of capital for some firms, and enhances price discovery and efficiency. On the other hand, the presence of lightly regulated exchanges can potentially have adverse spillover implications for a country’s other financial markets.

Attachment and social bonding in maximum and minimum security prison inmates

Attitudes towards social attachment and bonding were evaluated in groups of maximum (N=48) and bonding were evaluated in groups of maximum (N=46) security prison inmates and compared with the attitudes of a group of 49 noncriminal college students. Three primary styles of attachment were considered: avoidant, ambivalent/anxious, and secure. It was predicted that the maximum security condition, a group hypothesized to contain a large number of lifestyle criminals, would evidence more of an avoidant style of attachment compared to minimum security inmates or a group of college student controls. Consistent with this hypothesis, the maximum security inmates exhibited greater avoidance in their attitudes towards relationships than subjects in the other two conditions. The implications of these results are discussed.     

Lifestyle criminality from a developmental standpoint

A theory which details the development of lifestyle criminality is discussed. The necessary (but not sufficient) early conditions for the development of later criminality involve improper resolution of three early developmental tasks which confront all children (social attachment, stimulus modulation, self-image). A child predisposed to later criminality by weak social bonding, a high need for excitement, and a negative self-image is made even more vulnerable when exposed to such additional influences as low verbal intelligence, a chaotic home environment, or antisocial peers. Despite the importance of these variables, however, it is the lifestyle choices one makes which determine later criminality.     

Contentious Politics and Democratization: Comparing the Impact of Social Movements on the Fall of Communism in Eastern Europe

In this paper, I identify and analyze the interaction between two processes - mobilization and bargaining - by which democratic challengers can transform political institutions, bringing together insights from the literatures on social movements, which tends to analyze movement emergence, and democratization, which tends to analyze the design of democratic institutions. I compare the impact of social movements in the fall of communism in Eastern Europe, cases that offer a valuable opportunity to extend the literature on contentious politics beyond its origins in the study of Western parliamentary democracies. The analysis directs attention to an under examined arena of political contestation, agenda setting, or the process by which the demands of social movements are translated into issues for governments. The paper argues that the traditional dichotomy between institutional and non-institutionalized contention has obscured the ways that democratic challengers not only pressure states from the outside but transform them through new forms of political participation. Finally, it considers alternative explanations and suggests new directions for comparative research across different settings and times.     

Friends,Cognition, and Delinquency: Proactive and Reactive Criminal Thinking as Mediators of the Peer Influence and Peer Selection Effects among Male Delinquents

The purpose of this study was to determine whether criminal thinking underpins peer influence and selection. It was predicted that proactive criminal thinking would mediate the peer influence effect (peers?→?offending) and reactive criminal thinking would mediate the peer selection effect (offending?→?peers). Participants were 1,170 male delinquent youth from the Pathways to Desistance study. The Moral Disengagement scale (proactive criminal thinking) and Peer Delinquent Behavior scale (peer delinquency) were cross-lagged to predict criminal offending, and the Weinberger Impulse Control scale (reactive criminal thinking) and criminal offending were cross-lagged to predict peer delinquency. Consistent with predictions, proactive but not reactive criminal thinking successfully mediated the peer?→?offending relationship and reactive but not proactive criminal thinking successfully mediated the offending?→?peer relationship. Whereas delinquent peer associations appear to promote proactive criminal thinking and peer influence, early criminal offending appears to promote reactive criminal thinking and peer selection.