High‐Resolution Melting (HRM) of Hypervariable Mitochondrial DNA Regions for Forensic Science

Forensic strategies commonly are proceeding by analysis of short tandem repeats (STRs); however, new additional strategies have been proposed for forensic science. Thus, this article standardized the high‐resolution melting (HRM) of DNA for forensic analyzes. For HRM, mitochondrial DNA (mtDNA) from eight individuals were extracted from mucosa swabs by DNAzol reagent, samples were amplified by PCR and submitted to HRM analysis to identify differences in hypervariable (HV) regions I and II. To confirm HRM, all PCR products were DNA sequencing. The data suggest that is possible discriminate DNA from different samples by HRM curves. Also, uncommon dual‐dissociation was identified in a single PCR product, increasing HRM analyzes by evaluation of melting peaks. Thus, HRM is accurate and useful to screening small differences in HVI and HVII regions from mtDNA and increase the efficiency of laboratory routines based on forensic genetics.     

Extraction and isolation of cannabinoids from marijuana seizures and characterization by 1H NMR allied to chemometric tools

Marijuana, a drug derived from the Cannabis sativa L. plant, is the world's most consumed illicit drug. In this paper, a total of 156 marijuana samples seized in the state of Espírito Santo (ES), Brazil were studied and analysed by proton nuclear magnetic resonance (¹H NMR) spectroscopy to identify the major cannabinoids present. A crude extract of all samples was purified using high performance liquid chromatography so that these compounds could serve as reference substances. Nine fractions were obtained and analysed by ¹H NMR and gas chromatography–mass spectrometry (GC–MS), with five presented cannabinoids. ?⁹-THC (Δ⁹-trans-tetrahydrocannabinol), ?⁹-THCA (?⁹-tetrahydrocannabinolic acid), ?⁸-THC (?⁸-tetrahydrocannabinol), 11-hydroxycannabinol, CBV (cannabivarin), and CBN (cannabinol) were found, and their chemical structures were confirmed by GC–MS. The latter compound was obtained with high purity (≈100%), while the others were obtained as less complex mixtures with purity higher than 75% (except for Δ⁸-THC). Principal component analysis (PCA) was used on the ¹H NMR spectra of the 156 samples, and it was found that the samples were grouped according to the months, differentiating into two groups (from July 2014 to January 2015 and from February 2015 to July 2015), where non-grouping was observed from four macro-regions of the ES state (North, Central, Metropolitan, and South). The chemical profile of the seized samples was correlated to the ¹H NMR spectrum of an isolated CBN sub-fraction, in which the group formed by samples seized in the year 2015 presented lower CBN content in the chemical composition. From the PCA score plot, two groups of samples were confirmed using the partial least squares discriminant analysis and orthogonal projections to latent structures classification methods.     

Vertical transfers and the appropriation of resources by the bureaucracy: the case of Brazilian state governments

The purpose of this study is to explore whether the transfers received by state governments in Brazil are being appropriated by the bureaucracy as wages. The wage differential between the public and private sectors was used as a measure of this appropriation, following the technique for wage decomposition proposed by Oaxaca. Our results show that transfers stimulate appropriation by the bureaucracy, but that private groups existing within the states contend for these resources. The results also indicate that the resources appropriated are distributed uniformly between the various groups composing the bureaucracy.     

Intimate partner violence against women. Does violence decrease after the entry of the alleged offender into the criminal justice system?

Intimate partner violence(IPV)is simultaneously assumed as a serious crime and a major public health issue,having recurrences as one of its main characteristics and,consequently,re-entries of some alleged offenders in the criminal justice system(CJS).The main goal of this study is to assess if in cases of female victims of IPV,violence decreases after the first entry of the alleged offender in the CJS.A retrospective study was performed based on the analysis of police reports of alleged cases of IPV during a 4-year period.The final sample(n=1488)was divided into two groups according to the number of entries in the CJS(single or multiple)followed by a comparative approach.Results suggest that violence decreases after the first entry of alleged offenders in the CJS.Re-entries were found in only 15.5%of the cases but they were accountable for 3.3 times more crimes on average.Besides,victims of recidivism presented more injuries and required more medical care.Thus,a small group of alleged offenders seems to be more violent and accountable for most of the IPV crimes registered in the CJS suggesting that regardless of legal sanctions aiming to deter violence,these measures may not be enough for a certain group of offenders.This study sustains the need for a predictive model to quantify the risk of repeated IPV cases within the Portuguese population.     

Assessment of methods of homicides in a Brazilian city: a preliminary study

Homicides in the city of Ribeir?o Preto, state of S?o Paulo, Brazil, were analyzed with regard to methods (firearms or others) and gender, in a retrospective study from 1993 to 1997. There was a progressive increase in the number of homicides, mainly after 1995, with an increment of 86.6% in 1997 in comparison to 1993. The incidence of homicides was higher among males, with firearms being the most common method in this group since 1993. In addition, a 10% increase in this method was observed since 1995, in association with a proportional reduction in the use of other methods. The incidence of homicides among females remained stable until 1996 and increased by 51.3% in 1997. Simultaneously, firearms have become the most common method among female victims (71.4%), pattern similar to that observed among males. Attention is drawn to the social and economic conditions and their relationships with drug traffic in the city.     

Autosomic STR population data in two Caribbean samples from Colombia

In this work, a sample of 124 unrelated individuals from San Andres Island and Santa Marta City (Colombia) was studied for the nine STRs included in the AmpFlSTR Profiler kit (CSF1PO, D3S1358, D5S818, D7S820, D13S317, FGA, TH01, TPOX and vWA). Although these two populations are geographically apart, San Andres is an Island in the middle of Caribbean Sea (about 480 miles northwest the Colombian mainland) and Santa Marta City located in the coast, exact test showed no differentiation between both population samples (P=0.39445+/-0.0805). Therefore, allele frequencies and parameters of forensic interest were estimated for the global sample.     

Y-chromosome STRs in an Antioquian (Colombia) population sample

Haplotype data were obtained from a sample of 777 unrelated male individuals from Antioquia Department (Colombia), for eight Y-chromosome STRs (DYS19, DYS385, DYS389 I, DYS389 II, DYS390, DYS391, DYS392 and DYS393). A total of 442 different haplotypes were identified of which 334 were represented only once in the database and the most frequent haplotype was found in 32 individuals. A high haplotype diversity was found (99.45%). Genetic distances were calculated using previously published haplotype data and the lowest values were found for the comparisons with samples of lberian origin.     

The cordial maid: representations of servants in Brazilian adaptations of Eça de Queirós

ABSTRACT

This article considers the ways in which recent Brazilian film and television adaptations of the works of Eça de Queirós portray relationships between servants and their employers. I argue that the adaptations exaggerate certain connections and forge altogether new ones in order to create or emphasise a sense of kinship between people of different social strata.

This newfound familiarity between employer and employee, in relationships that recall Gilberto Freyre’s and Sérgio Buarque de Holanda’s theorisation on Brazilian society, breaks some of the fixed hierarchies presented in Eça’s work. Such a change, paradoxically, grants working-class characters much more air time than their original creator would have envisioned, while at the same time emptying the social commentary that derived from the rigid power structures found in the novels. In this context, this article argues that the blurring of class lines in these films and series is quintessentially Brazilian in its use of emotional connection to mask oppression and create a false sense of equality.