Tri-allelic SNP markers enable analysis of mixed and degraded DNA samples

For the analysis of degraded DNA in disaster victim identification (DVI) and criminal investigations, single nucleotide polymorphisms (SNPs) have been recognized as promising markers mainly because they can be analyzed in short sized amplicons. Most SNPs are bi-allelic and are thereby ineffective to detect mixtures, which may lead to incorrect genotyping. We developed an algorithm to find non-binary (i.e. tri-allelic or tetra-allelic) SNPs in the NCBI dbSNP database. We selected 31 potential tri-allelic SNPs with a minor allele frequency of at least 10%. The tri-allelic nature was confirmed for 15 SNPs residing on 14 different chromosomes. Multiplex SNaPshot™ assays were developed, and the allele frequencies of 16 SNPs were determined among 153 Dutch and 111 Netherlands Antilles reference samples. Using these multiplex SNP assays, the presence of a mixture of two DNA samples in a ratio up to 1:8 could be recognized reliably. Furthermore, we compared the genotyping efficiency of the tri-allelic SNP markers and short tandem repeat (STR) markers by analyzing artificially degraded DNA and DNA from 30 approximately 500-year-old bone and molar samples. In both types of degraded DNA samples, the larger sized STR amplicons failed to amplify whereas the tri-allelic SNP markers still provided valuable information. In conclusion, tri-allelic SNP markers are suited for the analysis of degraded DNA and enable the detection of a second DNA source in a sample.     

Measurement bias in self-reports of offending: a systematic review of experiments

Journal of Experimental Criminology - Self-reported offending is one of the primary measurement methods in criminology. In this article, we aimed to systematically review the experimental evidence...     

Presidents and cabinets: The political determinants of fiscal behavior in Latin America

What political factors drive fiscal behavior in Latin America’s persidential democracies? This work seeks to identify the political determinants of the level of public spending and the primary balance of ten democratic regimes in Latin America between 1980 and 1998. We consider, besides the influence of traditional variables such as the government’s ideological orientation and electoral cycle, the impact of other institutional and political aspects, such as the legislative strength of the president, ministerial stability, and the degree of centralization of budget institutions. Methodologically, the work is based on a pooled cross-section-time-series data analysis of 132 observations. Our main findings are that presidents supported by a strong party and leading a stable team of ministers—and ones more to the right on the political spectrum—had a negative impact on public spending and a positive effect on fiscal balance, and that the electoral cycle deteriorates the latter.     

Traditional Excluding Forces: A Review of the Quantitative Literature on the Economic Situation of Indigenous Peoples,Afro‐descendants,and People Living with Disability

Unequal income distribution in Latin America and the Caribbean is linked to unequal distributions of human and physical assets and differential access to markets and services. These circumstances, and the accompanying social tensions, need to be understood in terms of traditional fragmenting forces; the sectors of the population that experience unfavorable outcomes are also recognized by characteristics such as ethnicity, race, gender, and physical disability. In addition to reviewing the general literature on social exclusion, this article surveys several more specific topics: relative deprivation (in land and housing, physical infrastructure, health and income); labor market issues, including access to labor markets in general, as well as informality, segregation, and discrimination; the transaction points of political representation, social protection, and violence; and areas in which analysis remains weak and avenues for further research in the region.     

Field experiments on dishonesty and stealing: what have we learned in the last 40 years?

Journal of Experimental Criminology - Field experiments combine the benefits of the experimental method and the study of human behavior in real-life settings, providing high internal and external...     

A Reappraisal of Developing Deciduous Tooth Length as an Estimate of Age in Human Immature Skeletal Remains

This study provides an update on a quantitative method for immature age estimation based on postnatal deciduous mandibular tooth length. Two known sex and age skeletal collections from Western Europe were sampled (n = 97). Linear regression models for age estimated were calculated for each individual tooth, each sex, and sex combined sample using classical calibration. Prediction errors, residuals, and percentage of individuals whose real age fell within the 95% prediction interval were calculated. The teeth which develop earlier in life, the incisors and the first molar, showed the greatest precision, while the canine showed the least. This method has greater applicability to archeological skeletons or to children in developing countries than for use in North American or European forensic contexts. The method can be applied to incomplete or poorly preserved remains of unknown sex, particularly when dental radiographs are not an option or when teeth have been removed from the alveolus or crypt.     

The social security earnings test and work incentives

The labor supply and benefit claiming incentives provided by the early retirement rules of the Social Security Old Age benefits program are of growing importance as the Normal Retirement Age (NRA) increases to 67, the labor force participation of Older Americans rises, and a variety of reforms to the Social Security system are considered. Any reform needs to take into account the effects and rationale of the Social Security Earnings Test and the Actuarial Adjustment Factor, which are likely to be widely misunderstood due to the relatively little attention paid by policymakers and researchers to the fact that Americans are willing to work while receiving benefits. We describe these incentives and emphasize that individuals who claim benefits before the NRA but continue to work, or return to the labor force, can reduce the early retirement penalty by suspending the collection of monthly benefits if they earn above the Earnings Test limit. We then argue that the Earnings Test can be distortionary and is costly to administer, and that these characteristics are inflated by the lack of information given to Older Americans regarding the consequences of working while receiving retirement benefits. We present results from statistical models of labor force exit behavior using data from the Health and Retirement Study showing the relevance of these incentives, and investigate the importance of informational asymmetries among beneficiaries regarding benefit withholding using a dynamic life‐cycle model of labor supply and benefit claiming. We then use the latter framework to compare the behavioral and welfare implications of a removal of the Earnings Test to the policy of providing more information regarding the Earnings Test and the adjustment of the rate of benefit pay to Older Americans. © 2007 by the Association for Public Policy Analysis and Management     

Thijs Van de Graaf and Benjamin K. Sovacool: Global Energy Politics

International Environmental Agreements: Politics, Law and Economics -     

The Salinity Crisis in Western Australia: A Case of Policy Paralysis

This article focuses on the Court government's development of a State Salinity Strategy during the 1990s which took nine years from the initial announcement to the final realease. Western Australia has 70 percent of the nation's dryland salinity, a figure widely regarded as representing a potential environmental disaster with significant flow-on economic and social impacts. The paralysis that has surrounded decision-making on this issue is examined as a case study in the lack of effectiveness of government policy-making capacity on the environment. The shortcomings of the Salinity Strategy examined in the article include a lack of a leadership role for government, inadequate resourcing and weaknesses in the supporting institutional arrangements.