The 2000-2001 GEP-ISFG Collaborative Exercise on mtDNA: assessing the cause of unsuccessful mtDNA PCR amplification of hair shaft samples

We report the results of Spanish and Portuguese working group (GEP) of International Society of Forensic Genetics (ISFG) Collaborative Exercise 2001-2002 on mitochondrial DNA (mtDNA) analysis. 64 laboratories from Spain, Portugal and several Latin-American countries participated in this quality control exercise. Five samples were sent to the participating laboratories, four blood stains (M1-M4) and a sample (M5) consisting of two hair shaft fragments. M4 was non-human (Felis catus) in origin; therefore, the capacity of the labs to identify the biological source of this sample was an integral part of the exercise. Some labs detected the non-human origin of M4 by carrying out immuno-diffussion techniques using antihuman serum, whereas others identified the specific animal origin by testing the sample against a set of animal antibodies or by means of the analysis of mtDNA regions (Cyt-b, 12S, and 16S genes). The results of the other three human blood stains (M1-M3) improved in relation to the last Collaborative Exercises but those related to hairs yielded a low rate of success which clearly contrasts with previous results. As a consequence of this, some labs performed additional analysis showing that the origin of this low efficiency was not the presence of inhibitors, but the low quantity of DNA present in these specific hair samples and the degradation.As a general conclusion the results emphasize the need of external proficiency testing as part of the accreditation procedure for the labs performing mtDNA analysis in forensic casework.     

Defining and measuring the underclass

Research on the underclass has been hampered by the absence of a clear definition of the term. In this article we develop an operational definition of the underclass that is consistent with the emphasis of most of the underclass literature on behavior rather than poverty. Using this definition, we anlyze data for all census tracts in the United States in 1980. According to our definition, about one percent of the U.S. population lived in “underclass areas” in 1980, and this group was overwhelmingly concentrated in urban areas. It was also disproportionately made up of minorities living in the older industrial cities of the Northeast.     

Comparative performance of the Phadebas® Forensic Press Test at room temperature and 37 °C for the detection of saliva stains on fabric exhibits

The Phadebas® Forensic Press Test (PFPT) is an enzyme-based colorimetric test used to visualise and locate latent saliva stains on forensic exhibits. The test relies upon the presence of the enzyme α-amylase which is present in high levels in saliva. Even though the optimal in vitro temperature for α-amylase activity is 37 °C, the PFPT manufacturer’s protocol specifies that the PFPT should be carried out at room temperature (RT). In this study, we compared the performance of the PFPT at RT and 37 °C using combinations of four fabric types (cotton, polyester, acrylic and a cotton/polyester blend), three saliva dilutions (neat, 1:10 and 1:100) and stains aged for four time periods (1 day, 1 week, 1 month and 3 months). The intensity of the PFPT colour reactions at RT and 37 °C were not statistically different across all fabric types, saliva concentrations and stain ages, indicating that maximum sensitivity and performance of the PFPT can be achieved at RT.     

Public Financial Management Reforms: The Role of Ipsas in Latin‐America

During the last 30 years, many public administration reforms promoted by New Public Management have been undertaken. These reforms have spread to Latin‐American countries and include changes in governmental accounting systems, where the implementation of International Public Sector Accounting Standards (IPSASs) has been a stimulus for modernization. This article aims to clarify the situation of IPSASs implementation in the Latin‐American context as well as the stimuli for and effects of their implementation. The analysis shows that there is an emerging international trend to adopt IPSASs in Latin‐American countries although at the same time, there are evident obstacles to achieving reform goals. In Colombia, reforms are still underway, and the usefulness of IPSASs to improve decision‐making at an organisational level cannot be evaluated. Meanwhile, in Peru, the modernization is more rhetorical than real, and many efforts remain to be made for the effective implementation. Copyright © 2016 John Wiley & Sons, Ltd.     

File-sharing and downloading: goldmine or minefield?

Legal context: Major copyright owners have been slow to rise to the challengepresented by illegal file-sharing and downloading. In recentyears, they have scored a number of significant Court victoriesagainst file-sharers, but the recent decision in Promusicaev Telefónica, in which the ECJ held that the right tothe protection of industrial property does not necessarily outweighthe right to privacy, indicates that rights holders may benefitfrom a more creative and co-operative approach to file-sharing. Key points: The author provides an analysis of the Promusicae decision,along with a summary of the statutory position in the UK. Thisis also accompanied by a review of the recent internationallitigation landscape on file-sharing and a review of currentattitudes towards addressing illegal filesharing, includingrecent proposals from the British Government and the EuropeanParliament and new models of co-operation between rights holdersand file-sharing service providers. Practical significance: The Promusicae decision will disappoint copyright owners. TheECJ decision left it to Member States to determine whether thereshould be an obligation to disclose personal data in order toprotect copyright, so long as the interpretation of the lawattempts to reconcile the parties' competing rights and principlesand demonstrates proportionality. Copyright owners will, therefore,have to tailor enforcement strategies to individual Member States.This will hamper their ability to take action against individualinfringers efficiently. In addition to litigating against infringers,however, they may benefit from the new models of co-operationbetween copyright owners and file-sharing services which areemerging.