Quantitative analysis of amplifiable DNA in tissues exposed to various environments using competitive PCR assays

Competitive PCR assays were established for the mitochondrial DNA hypervariable region I and the human amelogenin locus. Using these assays, the copy numbers of DNA participating in PCR (amplifiable DNA) were quantified in tissues exposed to different environments. Human ribs, skin and nails were left in three exposure conditions (in the open air, in soil and in water). The amounts of amplifiable DNA in these tissues were quantified during a time period of up to two months. The amount of amplifiable DNA was well preserved in hard tissues (ribs and nails) regardless of the exposure conditions, whereas the soft tissues immersed in water showed a rapid decrease in amplifiable DNA. Strong PCR inhibition was observed in the DNA extracts obtained from buried bones. This phenomenon was clearly identified from an amplification failure of the internal standards in the competitive PCR. A preliminary examination to identify the PCR inhibitor suggested that the soil itself contributed to the inhibition. In addition, the amounts of amplifiable DNA in case samples were also investigated.     

What Is Critical Globalization Studies?

From a critical perspective, precisely what kind of knowledge about globalization is meaningful? The distinctive province of a critical orientation to globalization is coming to be defined by a complex of five interacting components: reflexivity, historicism, decentering, crossovers between social inquiry and other streams of knowledge, and an emphasis on strategic transformations. Critical globalization studies may be employed to identify diverse tendencies in world order, parts of a contradictory whole that coexist, with different logics colliding with one another. These are elements of the old configuration, multilateral globalization ; the contemporary structure, militarized globalization ; and the potential constellation, democratic globalization . The motor of transformation is not only countervailing power but also alternative knowledge, which should be demystifying and enabling.     

PROFESSIONAL INTERDEPENDENCE IN DIVORCE PRACTICES

Divorce mediation is one area of the new and growing field of interpersonal mediation. Differences and similarities between divorce mediation and divorce psychotherapy are discussed in this article, and a method for teamwork between the two professions is proposed. The authors take the approach that the separate but overlapping practices of mediation and psychotherapy can be interdependent and that specific benefits to divorcing clients can result when divorce mediation and divorce psychotherapy are practiced collaboratively.