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991.
Legrand B Mazancourt Pd Durigon M Khalifat V Crainic K 《Forensic science international》2002,125(2-3):205-211
Formalin-induced DNA degradation was studied at different fixation times (3, 7, 16 and 32 days) each on 10 formalin fixed paraffin embedded tissues (FFPET) stored for 15 years at room temperature.The four different extraction protocols used in this study showed that Chelex100 extracts performed the best at 3 and 7 days of formalin fixation (DFF) (with regard to the quantity and the quality of the DNA). However, Qiamp extracts showed better results for long sized alleles, as well for single polymerase chain reaction (PCR) amplifications after 16 and 32 DFF, as for multiplex PCR at shorter fixation times. DNA degradation is expressed by the size of the amplified alleles, only 100 bp templates surviving after 32 DFF (AMG locus). Single locus amplifications (CD4 and FES/FPS alleles) performed better than multiplex PCR (ProfilerPlus), with nearly 100% positive results at 7 DFF. In both types of amplifications, the success rate decreased proportionally with the time of formalin fixation and, consequently, with the size of the required DNA template. 相似文献
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Recent theoretical debates have questioned the compatibility of patriotism with global political responsibilities, as identified by cosmopolitan theory. In response, several authors claim that a cosmopolitan patriotism is both possible and desirable. In this article, we propose two desiderata for cosmopolitan patriotism as a civic ideal, which existing accounts fail to meet. First, arguments for cosmopolitan patriotism should provide an account of collective identification, supporting the relation between the actions of one's country and one's appropriate reactive attitudes. Second, such a theory should be able to explain the patriot's commitment to critical engagement with her country's actions. We then offer a critical appraisal of two accounts linking patriotism with global responsibility—Permissible Partialism and Globally Responsible Nationalism—-and demonstrate how they fall short. Finally, we propose an account of civic republican patriotism, which better explains how cosmopolitanism and patriotism can be brought together. 相似文献
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