保存温度与时间对生物样品中氯胺酮稳定性的影响

目的研究不同温度和时间条件保存时生物样品中氯胺酮的稳定性。方法家兔以氯胺酮150mg/kg灌胃,30min后处死,取其血、肝、肾、脑,分别在室温（18～24℃）和冷冻（-20℃）条件下保存,并用气相色谱-质谱法定性分析、气相色谱-氮磷检测器法测定不同时间各样品中氯胺酮含量。结果血、肝、肾、脑冷冻保存至第30天氯胺酮含量均降低（P〈0.05）;室温条件下各样品中氯胺酮含量自第5天起均升高（P〈0.05）。结论生物样品在冷冻条件下保存时氯胺酮稳定性较好,怀疑氯胺酮中毒或死亡的检材应冷冻保存,尽快检测。     

病毒性心肌炎猝死心肌MCP-1表达及其法医学意义

目的探讨单核细胞趋化蛋白1（monocyte chemoattractant protein-1,MCP-1）在病毒性心肌炎（viral myocarditis,VMC）所致猝死者心肌组织中的表达及其意义。方法运用改良的免疫组织化学方法,观察VMC猝死组和对照组心肌组织中MCP-1的表达情况,应用统计学处理,比较两组间的表达差异。结果20例VMC猝死组中有17例MCP-1染色阳性;而20例非VMC猝死对照组仅4例局部散在MCP-1弱阳性表达,其余均阴性。VMC组MCP-1阳性表达率明显高于对照组（P〈0.01）。结论MCP-1的免疫组织化学检测可作为法医病理学诊断VMC所致猝死较为客观的病理形态学指标之一。     

近年来国家文化安全热点问题分析

近年来,围绕我国国家文化安全显示出诸多热点问题,值得我们关注与深思:国家文化软实力快速提升,民族精神空前升华,"国学热"不断升温,"汉语热"持续火爆,传统节日调整备受关注,山寨文化迅猛崛起等等,无一不表明我国国家文化安全已经呈现出与时俱进的崭新态势。     

论美国言论自由判例中的公共论坛原理——从2009年萨姆案谈起

公共论坛是指在传统上供人们聚集在一起以表达思想和交流观点的公共场所。美国联邦最高法院在言论自由司法判例中形成了公共论坛原理,该原理的本质意义在于明确公民在什么程度上有权为了表达的目的使用政府地产,而政府规制公民在公共地产上发表言论的权力限度又在哪里。这一原理近年来得到了进一步发展,2009年萨姆案赋予了公共论坛原理新的内容。公共论坛原理对于中国法治建设具有启发和借鉴意义。     

论土地征收过程中失地农民权益保护问题

在我国,随着城市化和工业化进程的加快,农民的土地大量被征收作为建设用地,失地农民越来越多。在土地征收过程中,失地农民权益受侵害的现象比较突出,失地农民群体上访或聚众闹事时有发生,严重影响了社会稳定。因此,探讨和解决失地农民的权益保护问题,对于农村社会的稳定、农民权益的保护、农业的发展具有重要的意义。     

马克思主义自然观及其多角度应用

自然是先于人类社会的客观存在,人靠自然界生活;人的实践活动使自然界打上了人的意志的烙印,使自然界"人化";从人类社会历史发展的角度把握自然界,自然界与人类社会不可分割;按照美的规律再生产自然界,实现人与自然的和谐.这种马克思主义的自然观一经形成就应用于其对各种具体问题的理论分析之中.     

我国公用事业特许经营政策变迁之述评

在国家政策和地方政策的引导下,我国公用事业特许经营改革稳步推进。政策主导型的特许运营模式虽然具有灵活应变的优势,但也存在预期缺乏的局限。为了实现我国公用事业特许经营模式向法制主导型模式的转变,必须在总结中央地方政策引导经验教训的基础上,实现中央与地方特许经营立法权限的科学配置。     

Influence of environmental variables on fear of crime: Comparing self-report data with physiological measures in an experimental design

Objectives

Self-reports and questionnaires have been the preferred research methods in the criminological field of “fear of crime” (FOC) since its rise in the 1960s. Our study had two main goals: (1) to measure the physiological indicators of fear in real time and (2) to compare these data with those obtained through self-reports, designed also to measure the emotion of fear.

Methods

An experimental study was conducted over the course of a week during late February 2016 in Aarhus (Denmark), in which the focus was on traditional environmental variables in the field of FOC (i.e., poor lighting conditions).

Results

Our results support the ideas that: (1) the absence of good luminosity in an open public space in an urban setting elicits physiological reactions of arousal that can be taken as indicators of experiences of fear and (2) heart rate appears to capture aspects of the emotion of fear that are not reflected in data obtained through self-report questionnaires.

Conclusions

This study, introducing a pioneering approach to the study of FOC, presents great potentials in complementing traditional methods in the crime sciences. The many challenges we faced are significant and reported with the hope that subsequent literature will build upon. We propose that traditional methods and new measurements could be combined to advance research in the field by allowing researchers to more unambiguously constrain the interpretation of their data. This becomes particularly relevant in a field like FOC, which has long suffered from irreconcilable results stemming from different investigations.

     

Y染色体OSU49基因座遗传多态性分析

目的调查Y染色体OSU49基因座在河南汉族群体中的遗传多态性,评价其法医学应用参数。方法知情同意情况下,采集300名河南汉族男性个体的血样本,荧光标记PCR,扩增产物采用ABI 3130遗传分析仪检测。根据分型检测结果,对不同等位基因进行序列分析(测序)。结果 OSU49基因座包含五核苷酸和四核苷酸两种核心序列。在河南汉族群体中基序表现为:(CTTTC)pCTT(CCCT)7T(CTTTC)1(TCTT)5(TCCT)m(TCTT)n TCT(TCCT)4,五核苷酸核心序列的重复次数为12~17,四核苷酸核心序列的重复次数为20~30,按其片段长度命名等位基因,共发现34个等位基因,GD值为0.918 6,DP值为0.915 5。结论 Y染色体OSU49基因座序列结构复杂,在河南汉族群体中具有较高的遗传多态性,可应用于法医学和人类遗传学研究中。     

Duplications of the Y-chromosome specific loci P25 and 92R7 and forensic implications

In the present study, we demonstrate that two commonly used Y-chromosome single nucleotide polymorphisms (SNPs), P25 and 92R7, are paralogous sequence variants (PSVs) originating from segmental duplications and that at least one of the sequence variants in each group of loci is polymorphic. Several methodologies were used in order to detect the SNP alleles and the PSVs of the loci. All results obtained with the various typing techniques supported the conclusion. The allele distributions of the binary markers were analysed in more than 600 males with seven different haplogroups. For P25, the ancestral allele C was found in several samples from different haplogroups. The derived allele A was always present with an additional C variant. Haplogroup P was defined by the derived allele A at the 92R7 locus. However, the ancestral allele G was always associated with an A variant due to the duplication.