Rapid isolation with Sep-Pak C18 cartridges and capillary gas chromatography of triazine herbicides in human body fluids.

A simple and rapid method, for the isolation of eight triazine herbicides from human serum and urine, using Sep-Pak C18 cartridges is presented. After mixing with distilled water, serum and urine samples containing the herbicides, were loaded on Sep-Pak C18 cartridges and eluted with either chloroform only or chloroform/methanol (9:1). The herbicides were detected by capillary gas chromatography with both flame ionization detection (FID) and nitrogen-phosphorus detection (NPD). Separation of eight triazine herbicides from each other and from impurities was generally satisfactory with the use of a non-polar DB-1 capillary column. Recovery of most compounds was excellent for both chloroform and chloroform/methanol (9:1) as elution solvents. Backgrounds were cleaner and evaporation time was shorter for the chloroform only than for the chloroform/methanol (9:1). The NPD gave sensitivity more than 10-20 times higher than that of FID.     

The detection of ephedrine by solid-phase immunoenzyme analytical and gas chromatographic methods

Comparative analysis of ephedrine derivative content in 96 urine specimen obtained from people suspected in abuse with these drugs. Physical and chemical methods of the analysis (gas and thin-layer chromatography) as well as ELISA were used. It was stated that the latter is specific for determination of compounds related to ephedrine by structure and it allows one to detect its analogues in 87 cases. Gas chromatography made it possible to detect ephedrine derivatives in 94 specimen tested and thin-layer chromatography--in 64 specimen tested. The given ELISA may be recommended for medicolegal expert purposes and narcology practice.     

An unusual death in water

A case of combined suicide involving the explosion of a handgranate in water demonstrates the possible misinterpretation of external injuries.     

Effects of a state law on rates of restraint on a child and adolescent unit

Rates of restraint and seclusion on a child and adolescent unit were evaluated before and after the implementation of a restrictive state law which was designed to reduce the monthly rates of restraint. Overall, the total number of hours in restraint, corrected for mean daily census, decreased significantly. The average number of patients in chemical restraint stayed about the same. There was a significant increase in number of patients, number of episodes, and hours of mechanical restraint as expected. Rates of seclusion dropped to zero as mandated. A new category of physical restraint was defined by law and was used to limited extent.     

The dynamic postmortem changes of the nucleoproteins in the tissues of the liver and eye

Postmortal changes in nucleoproteins were examined in the hepatocytes and anterior corneal and crystalline lenticular epithelial cells from 106 cadavers within 48 h after death by microfluorometry. Cryostat tissue sections were stained by acridine yellow by the method of R. Rigler (1966). The author found a natural decrease in fluorescence intensity of the examined tissue cellular nuclei at 530 nm within 4-24 h. and an increase in their fluorescence intensity at 640 nm within 4-48 h. after death. Data obtained may be used to establish the time of death.     

The clinico-morphological basis for a diagnosis of diffuse axonal brain damage in head trauma

The most typical neurologic signs and variants of computer-tomographic cerebral picture in diffuse axonal lesion of the brain are presented. Dynamics of pathomorphologic changes depending on duration of posttraumatic periods is detected.