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591.
Abstract: Low concentrations of microbial pathogens in pure and mixed samples were detected using a bead‐based, liquid array technology. A 20‐bp sequence in the 23S rRNA gene, rrl, was amplified in four microorganisms: Bacillus cereus, Escherichia coli, Salmonella enterica and Staphylococcus aureus. PCR products were positively identified with the Luminex® 100? system. The system could detect very low amounts of DNA and the instrument response was proportional to the input concentration. The lower limit of detection (LLD) was determined to be 0.5 ng for B. cereus and E. coli and 2 ng for S. enterica. The LLD for S. aureus was not determined as the instrument response was still above the threshold when quantities of DNA as low as 0.25 ng were used. The platform positively identified organisms present in mixed samples even when the minor component was overshadowed by a 10‐fold excess of the major component. 相似文献
592.
Cooley-Strickland MR Griffin RS Darney D Otte K Ko J 《Journal of prevention & intervention in the community》2011,39(2):149-166
This study evaluated the efficacy of a school-based anxiety prevention program among urban children exposed to community violence. Students who attended Title 1 public elementary schools were screened. Ninety-eight 3rd-5th-grade students (ages 8-12; 48% female; 92% African American) were randomized into preventive intervention versus wait list comparison groups. Students attended 13 biweekly one-hour group sessions of a modified version of FRIENDS, a cognitive-behavioral anxiety intervention program. Results indicated that both intervention and control groups manifested significant reductions in anxiety symptomatology and total exposure to community violence, along with improved standardized reading achievement scores. Additional gains observed only in the intervention group were increased standardized mathematics achievement scores, decreased life stressors, and reduced victimization by community violence. The intervention was equally efficacious for both genders and for children exposed to higher, compared to lower, levels of community violence. Implications for comprehensive, culturally and contextually relevant prevention programs and research are discussed. 相似文献
593.
Katherine A. Roberts Ph.D. Donald J. Johnson M.S. Sherillelynn Cruz M.S. Heather Simpson M.S. Alan Safer Ph.D. 《Journal of forensic sciences》2014,59(4):909-918
This study examined whether flossing the teeth is a more effective collection method in recovering spermatozoa than conventional swabbing techniques. It was hypothesized that inclusion of flossing as a collection method would extend the recovery of spermatozoa to longer postcoital intervals (PCIs). Eighteen individuals provided 174 oral cavity samples. Successful recovery of spermatozoa was assessed with respect to the collection method and reported activity in the oral cavity during the PCI. Samples were subjected to a differential extraction procedure prior to microscopic evaluation of the extracted pellet. The results indicate that swabbing is more effective than flossing when the PCI falls within 1.5–12 h. However, spermatozoa were recovered from seven floss samples where the corresponding swabs gave negative results. When combining the results from the two collection methods, the percentage of subjects from whom spermatozoa are recovered increases for each PCI beyond the 0‐h interval. 相似文献
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Timothy Caulfield Sarah Burningham Yann Joly Zubin Master Mahsa Shabani Pascal Borry Allan Becker Michael Burgess Kathryn Calder Christine Critchley Kelly Edwards Stephanie M. Fullerton Herbert Gottweis Robyn Hyde-Lay Judy Illes Rosario Isasi Kazuto Kato Jane Kaye Bartha Knoppers John Lynch Amy McGuire Eric Meslin Dianne Nicol Kieran O’Doherty Ubaka Ogbogu Margaret Otlowski Daryl Pullman Nola Ries Chris Scott Malcolm Sears Helen Wallace Ma'n H. Zawati 《Journal of Law and the Biosciences》2014,1(1):94-110
596.
This article presents the results of a pilot study on the effects of vulture modification to human remains. A donated body from the Willed Body Donation Program was placed at the Forensic Anthropology Research Facility (FARF), an outdoor human decomposition laboratory located at Texas State University-San Marcos. The effects of vulture scavenging on the timing and sequence, and the rate of skeletonization, disarticulation, and dispersal were observed via a motion sensing camera and direct observation. Using GIS (Geographic Information Systems) and GPS (Global Positioning System) technologies and spatial analytical methods, the transport of skeletal elements was mapped in order to analyze dispersal and terrain-influenced patterns of active vulture scavenging. Results showed that the initial scavenging took place 37 days after placement at FARF. This delay in scavenging differs from previous research. After the initial appearance of the vultures, the body was reduced from a fully-fleshed individual to a skeleton within only 5h. This underscores the potential for errors in postmortem interval estimations made at vulture scavenged scenes. Additionally, spatial analysis showed that skeletal elements were dispersed by vultures to lower elevations, and that the disarticulation and dispersal of the skeletal elements occurs early in the scavenging sequence. 相似文献
597.
In human social interactions, punishment is often directed at cheating individuals. Subjective reports and neuro-imaging studies indicate that the experience of interacting with a cheat produces negative emotions and that the act of punishing a cheat assuages these feelings. However, while negative emotions may elicit punishment, the precise source of these emotions remains obscure. Specifically, it is often very difficult to tease apart whether punishing individuals are inequity averse (upset because cheating partner receives more than they should) or, more simply, whether they might be loss averse (upset because their payoffs did not meet their expectations). We compare results on punishment and inequity aversion in humans with results from a non-human model system, the cleaning mutualism between bluestreak cleaner wrasse (Labroides dimidiatus) and its reef-fish ??clients??. Male cleaner fish are known to punish females that cheat during joint client inspections, but a recent study failed to demonstrate evidence for inequity aversion in this species. We suggest that punishment in cleaner fish may be motivated by loss aversion rather than inequity aversion. Punishment in humans might also often be motivated by loss aversion??and empirical studies that disentangle the two competing motives for punishment are a clear research priority. 相似文献
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Marsha Kline Pruett Glendessa M. Insabella Katherine Gustafson 《Family Court Review》2005,43(1):38-51
The role the legal process of separation and divorce plays in affecting outcomes for young children and their families was examined in the Collaborative Divorce Project (CDP), an intervention designed to assist the parents of children six years old or younger as they begin the separation/divorce process (married and unmarried couples). Evaluation and outcome data were collected from 161 couples, their attorneys, teachers, and court records. In addition to positive evaluations from both parents, intervention families benefited through lower conflict, greater father involvement, and better outcomes for children than the control group. Attorneys and court records indicate that intervention families were more cooperative and were less likely to need custody evaluations and other costly services. The CDP illustrates how prevention programs can be located within the courts, can be systematically evaluated, and can aid in helping the legal system function optimally for families with young children. 相似文献