Using FastID to analyze complex SNP mixtures from indoor dust

Forensically relevant single nucleotide polymorphisms (SNPs) can provide valuable supplemental information to short tandem repeats (STRs) for investigative leads, and genotyping can now be streamlined using massively parallel sequencing (MPS). Dust is an attractive evidence source, as it accumulates on undisturbed surfaces, often is overlooked by perpetrators, and contains sufficient human DNA for analysis. To assess whether SNPs genotyped from indoor dust using MPS could be used to detect known household occupants, 13 households were recruited and provided buccal samples from each occupant and dust from five predefined indoor locations. Thermo Fisher Scientific Precision ID Identity and Ancestry Panels were utilized for SNP genotyping, and sequencing was completed using Illumina^® chemistry. FastID, a software developed to permit mixture analysis and identity searching, was used to assess whether known occupants could be detected from associated household dust samples. A modified “subtraction” method was also used in FastID to estimate the percentage of alleles in each dust sample contributed by known and unknown occupants. On average, 72% of autosomal SNPs were recovered from dust samples. When using FastID, (a) 93% of known occupants were detected in at least one indoor dust sample and could not be excluded as contributors to the mixture, and (b) non-contributor alleles were detected in 54% of dust samples (29 ± 11 alleles per dust sample). Overall, this study highlights the potential of analyzing human DNA present in indoor dust to detect known household occupants, which could be valuable for investigative leads.     

Legal Invisibility and the Revolution: Statelessness in Egypt

Recent political turmoil has focused international attention on Egypt, yet there is little awareness of the country’s stateless populations—those who lack legal nationality to any state—or the challenges they face. Individuals in situations of protracted statelessness are denied their right to a nationality, resulting in an array of additional rights violations. Such violations include denied freedom of movement, equality before the law, and access to economic and social rights. Drawing from two years’ of fieldwork data, this study highlights the plight of those who are unable to achieve legal status in a country with harsh punishments for illegal presence, entry, and exit. It also evaluates potential solutions for eliminating statelessness in Egypt and protecting the rights of stateless populations. As Egypt seeks to move beyond revolution, it is vital that the government addresses the pervasive and systemic inequalities that deny individuals their right to a nationality.     

Assessing three soil removal methods for environmental DNA analysis of mock forensic geology evidence

Soil is useful in criminal investigations as it is highly variable and readily transferred. Forensic geologists use several different techniques to removal soil from evidence prior to the analysis of inorganic components. There has been recent interest from the forensic science community to analyze environmental deoxyribonucleic acid (eDNA) associated with soil to augment existing forensic analyses. Notably however, limited research has been conducted to compare commonly used soil removal methods for downstream eDNA analysis. In this study, three soil removal methods were assessed: picking/scraping, sonication, and swabbing. Three mock evidence types (t-shirts, boot soles, and trowels) were sampled in triplicate with each removal method (n = 27). Soil samples underwent DNA isolation, quantification, and amplification of four genomic barcode regions: 16S for bacteria, ITS1 for fungi, ITS2 for plants, and COI for arthropods. Amplicons were prepared into libraries for DNA sequencing on an Illumina^® MiniSeq. DNA concentrations were highest in picked/scraped samples and were statistically significant compared with swabbed and sonicated samples. Amplicon sequence variants (ASVs) were identified, and removal methods had no impact on the recovery of the total number of target ASVs. Additionally, when assessing each sample in multidimensional space, picked/scraped samples tended to cluster separately from swabbed and sonicated samples. The soil core used a reference in this study also clustered with the picked/scraped samples, indicating that these samples may be more reflective of the communities collected from soil cores. Based on these data, we identified that picking/scraping is an acceptable soil removal method for eDNA analysis.     

What Should We Do? Public Attitudes about How Local Government Officials Should Confront Fiscal Stress

Despite strong scholarly interest in the topic of fiscal stress, little attention has been paid to understanding how the general public thinks local governments should respond to situations where declining revenues endanger service levels. This study reports findings from a survey of 660 residents undertaken between November 2006 and January 2007 in the US state of Michigan to examine their support for eight potential strategies to cope with fiscal stress in five different local government services. We find that the public has a surprisingly nuanced perspective about these strategies and on their use for different services. Our findings may provide local policymakers with some insights about how to respond to fiscal stress.     

The cultural labor of surveillance: video forensics,computational objectivity,and the production of visual evidence

This essay argues that the status of video evidence as an index of real events—a sign or representation that offers a direct, empirical connection to material reality—is the result of an intentional process of production. This process involves the repurposing of new technologies borrowed from the domain of creative media production in order to transform a chaotic field of raw surveillance video into useable evidence. In addition to the exchange in technologies, an unavoidable epistemological and interpretive exchange takes place between evidentiary uses of surveillance video on the one hand, and the now prevalent forms of surveillant narration found in both fictional and reality-based storytelling. But despite this exchange in meanings and technical systems, considerable effort has gone in to establish formal standards for the evidentiary uses of surveillance video that distinguish the discovery of video evidence from the production of creative content. Building on Daston and Gallison's historical study of the prevailing “epistemic virtues” that have defined objectivity over time, I argue that what we see emerging in the field of forensic video analysis, as a means of establishing its scientific and legal status, is a commitment to a new epistemic virtue of “computational objectivity.”     

Antitrust policy in a hostile environment: institution building in venezuela's procompetencia

Theories of bureaucracy in Latin America generally stress institutional weakness, political volatility and the politicized nature of government agencies. One product of market liberalization in Venezuela was the creation of the anti-monopoly agency called “Procompetencia”, which managed to survive a change of government and a move toward interventionist, anti-market mechanisms hostile to its survival. The strategy and tactics of this agency are reviewed with the objective of detecting bureaucratic responses to threat and successful efforts to survive. Some lessons are derived for infusing more stability into weak institutions.     

Using Paint to Investigate Fires: An ATR‐IR Study of the Degradation of Paint Samples Upon Heating

Fire investigation is a challenging area for the forensic investigator. The aim of this work was to use spectral changes to paint samples to estimate the temperatures to which a paint has been heated. Five paint samples (one clay paint, two car paints, one metallic paint, and one matt emulsion) have been fully characterized by a combination of attenuated total reflectance Fourier transform infrared (ATR‐IR), Raman, X‐ray fluorescence spectroscopy and powder X‐ray diffraction. The thermal decomposition of these paints has been investigated by means of ATR‐IR and thermal gravimetric analysis. Clear temperature markers are observed in the ATR‐IR spectra namely: loss of ν(C = O) band, >300°C; appearance of water bands on cooling, >500°C; alterations to ν(Si–O) bands due to dehydration of silicate clays, >700°C; diminution of ν(CO₃) and δ(CO₃) modes of CaCO₃, >950°C. We suggest the possible use of portable ATR‐IR for nondestructive, in situ analysis of paints.     

Carbon Monoxide Poisoning and Death in a Large Enclosed Ventilated Area

A 55‐year‐old man with a medical history of tobacco use suddenly collapsed while power washing an empty indoor pool in a hotel. The decedent was transported to the local hospital where he was pronounced. A postmortem examination revealed atherosclerotic heart disease and bilateral pulmonary edema and congestion. A postmortem blood carbon monoxide (CO) level was 27% saturation, and a CO performed on hospital admission blood was 49% saturation. CO poisoning is a common cause of toxicological morbidity and mortality in the United States. The circumstances most often occur in an enclosed environment and may be intentional or unintentional. CO poisoning has been reported in open, well‐ventilated spaces, but rarely results in death. A warning label was present on the engine clearly stating the dangers of CO emission. However, there was a false sense of security due to the large size of the pool room and the presence of industrial blowers that were being used for ventilation.     

Slaves as criminal informers in ancient Rome

The Romans expected slaves to inform on their fellow slaves, particularly in regard to the master's safety. If a slave killed his master, a terrible retribution on the entire household of slaves would have to be paid to atone for the offense. In the final analysis, the slave‐informer system, with all of its drawbacks, supported the Roman criminal justice system nearly 1,000 years and the Byzantines employed it in a modified form. This paper is a case study of how a culture, with its values, musters its resources, in the case of slavery, to cope with the problems of internal security.

With most of the crime problems that confront us today, the ancient Romans were hard put to protect the public peace. They had neither the modern technology nor the forensic science that we do, so they utilized the limited resources they had to prevent crimes and catch criminals. They devised a policy of enticing slaves to act as criminal informants with the offer of freedom. Considering the sizeable adult slave population, this policy greatly deterred crime and the saying “Every slave, an enemy” became current among the citizenry.

The Romans, however, did not intend to open a door by which slaves could accuse citizens of false charges out of vindictiveness. The legal‐minded Romans adopted the safeguard of having the slave informant interrogated under torture.

Slaves also served the criminal justice system in other ways besides volunteering information. When the police were stymied in an investigation, they “rounded up the usual suspects” in the form of slaves who might have knowledge of the crime, but for some reason had not stepped forward. The police would use their powers to interrogate slaves in this case, too. This ancient practice reflects the procedure of modern police investigators checking surveillance cameras in the vicinity of the crime scene in the hopes of discovering a clue.