When using non-FTA cards in commercial multiplex STR kits for direct PCR, pretreatment steps with specific buffers are recommended. Here, we designed a rapid direct PCR method utilizing a non-FTA card, Oral Cell Sampling Kit, by omitting the pretreatment step involving Prep-n-Go™ Buffer, and it showed compatibility with the GlobalFiler™ Express PCR Amplification Kit, GlobalFiler™ PCR Amplification Kit, and PowerPlex® Fusion system. To optimize the PCR conditions, we tested the method with different final PCR volumes and cycles. Finally, we conducted a performance test using 50 Korean buccal samples and confirmed the high performance of the method, detecting more than 90% of the samples with full profiles when using GlobalFiler™ PCR Amplification Kit and PowerPlex® Fusion system at 29 cycles in a 10 μL final PCR volume. Thus, we report a simple direct PCR set-up to analyze reference samples collected using a non-FTA card manufactured in Korea. 相似文献
Journal of Youth and Adolescence - Language brokering is a prevalent phenomenon in ethnic minority immigrant populations. Although accruing evidence points to the beneficial impacts of healthy role... 相似文献
Research on the health benefits and consequences of close relationships has suggested the linkage in daily emotions (i.e., coregulation) between close partners is an important relationship dynamic. While the coupling of daily emotions among family members (parent–child and marital dyads) has been widely documented, research examining emotional coregulation among ethnic minority youth during adolescence, a period marked by heightened emotion and risk for psychopathology, remains an important area in need of exploration. This study examined correlates of emotional coregulation in a sample of Mexican-origin adolescents (Mage?=?15.02, SD?=?.83) and their parents (Mage?=?41.93, SD?=?6.70). Dyads reported on daily levels of distress and happiness for 14 consecutive days across two waves of data collection a year apart (nwave1?=?428 dyads, nwave2?=?336 dyads). Dyads who reported getting along were more likely to coregulate their daily happiness. Importantly, coregulation of distress was only present in older adolescents who reported above average levels of internalizing symptoms. The results suggest coregulation of distress may shape or be shaped by poor mental health during the later years of adolescence, a time when youth may be establishing a degree of emotional autonomy from parents.
This study provides a systematic review of the development of Chinese public administration in English language journals. An analysis of articles in the top 25 English-language public administration journals worldwide from 1996 to 2016 confirmed increases in both the number and significance of studies of Chinese public administration. A systematic content analysis of abstracts of previous studies was performed and showed that social development and administrative reform were among the most important topics. With respect to the methodology of this study, qualitative methods were more frequently used than quantitative or mixed methods. Finally, implications for future research on Chinese public administration are discussed. 相似文献
Organizational culture is widely considered to be one of the most significant factors in reforming and modernizing public administration and service delivery. This article documents the findings of a literature review of existing qualitative and quantitative instruments for the exploration of organizational culture. Seventy instruments are identified, of which 48 could be submitted to psychometric assessment. The majority of these are at a preliminary stage of development. The study's conclusion is that there is no ideal instrument for cultural exploration. The degree to which any measure is seen as "fit for purpose" depends on the particular reason for which it is to be used and the context within which it is to be applied. 相似文献
In this paper, we examine whether the impact of negative advertising on citizens’ evaluations of candidates depends on the
gender of the candidates. Given common gender stereotypes, we expect negative campaigning aimed at women candidates will affect
citizens differently than negative campaigning against male candidates. The results of our study, derived from a survey experiment
conducted on a nationwide sample of more than 700 citizens, demonstrate that negative commercials are less effective at depressing
evaluations of woman candidates, compared to male candidates. The findings are consistent and strong, across a range of forces
that people use to assess competing candidates (i.e., affect and trait evaluations, people’s beliefs about issues, anticipated
vote choice). The tight control of the experimental design, including randomization of respondents into different conditions
that vary in only one way, demonstrates that the gender of the candidate influences people’s reactions to different types
of negative commercials.
Conscious policy and program support for the resettlement of refugees in regional and rural areas is a relatively recent trend in Australia. Resettlement is a complex process that hinges on the establishment of viable communities. This review suggests that its outcomes, especially for refugee communities, are potentially mixed. However, an appropriately resourced, well managed and well planned refugee resettlement program can potentially provide beneficial outcomes for refugees and host communities. This article draws on existing empirical data and contributions made at a VicHealth Roundtable in late 2007 to thematically explore the challenges facing rural and regional resettlement programs. Given that settlement is a dynamic process and the approaches to rural and regional settlement are evolving, this article provides 12 propositions for a more effective and integrated approach to policy and practice. 相似文献
This essay explores how South Koreans have creatively acculturated the meaning of citizenship using Confucianism-originated familial affectionate sentiments (ch?ng), while resisting a liberal individualistic conception of citizenship, by investigating contemporary nationalist politics in South Korea. Its central claim is that the ch?ng-induced politico-cultural practice of collective moral responsibility (uri-responsibility), which transcends the binary of individualism and collectivism and of liberalism and nationalism, represents the essence of Korean national citizenship. In other words, this essay attempts to make a Korean case of “liberal nationalism” in its post-Confucian context. 相似文献
With its strategic location in East Asia, the Yellow Sea Rim is becoming an important economic region, where the socialist
economies of China and North Korea and the market economies of Japan and South Korea interact and exchange for their mutual
benefit. In light of the recent rapprochement among Northeast Asian countries, the article describes the emerging pattern
of development in the Yellow Sea Rim. It assesses the potential gains of opening up the region by assuming a complete or partial
removal of political barriers. Anticipating the future of cooperative regional development in the Yellow Sea Rimlands, the
article discusses regional strategies and key issues involved in cooperation, in particular between China and South Korea. 相似文献