Sudden infant death due to asplenia syndrome

An apparently healthy 80-day-old boy died suddenly for no apparent reason. The autopsy revealed that the patient had had congenital asplenia, extensive cardiovascular anomalies, and other organ malformations, including trisegmented lungs, hypoplasia of the corpus callosum and cranial bones, a symmetrical liver, accessory hepatic tissue in the adrenal glands, malrotation of the intestine, and hypoplasia of the greater omentum.     

Lewis blood group determination in bloodstains by planimetric measurement of eluted monoclonal antibodies

Planimetric measurements were employed for reading the results of an elution test to determine Lewis blood groups in dry human bloodstains. In the absorption-elution test, two varieties of indicators were used to detect eluted Lewis antibodies. First, 64 blood-stains aged between 2 to 8 months were tested with glutaraldehyde (GLA)-treated erythrocytes (planimetric hemagglutination assay, PMHA). This method demonstrated that dry stains weighing approximately 0.4 mg (equivalent to 3 microliters of whole blood) were sufficient for detection of Lea or Leb antigen. Results were obtained within 1 h. Then, 37 of these stains were tested with Lewis substance-coated latex particles (planimetric latex agglutination assay, PMLA). The presence of Lea and Leb antigen were detected from dry stains weighing 0.1 mg (equivalent to 1 microL of whole blood) within 3 h. Both these assays are faster and simpler with accuracy than the enzyme-linked immunosorbent assay (ELISA). Latex particles coated with Lewis substance are, in particular, strongly agglutinated and show agglutination patterns more clearly than erythrocytes. The blind tests using these two methods properly classified 7 Le(a + b-) and 23 Le(a-b + ) bloodstains; whereas, 5 Le(a-b-) stains were undetermined by the criteria for these tests. These results indicate the usefulness of the PMHA and PMLA for typing Lewis blood groups from small bloodstains.     

Detection of human chorionic gonadotropin (HCG) in fabric urine stains older than five years

Human chorionic gonadotropin (HCG) is used in clinical medicine as a particularly important indicator to determine pregnancy. In this study, it was necessary to determine whether the urine spots on car seat fabric from a murder 5 years previously were from a pregnant woman. The HCG in the dried urine spot on a car seat was detected using an immunochromatography kit. It was found that the HCG in urine can be detected for much longer periods of time than the previously reported period of approximately 6 months.     

Differences in tissue distribution of HV2 length heteroplasmy in mitochondrial DNA between mothers and children

Sequence analysis of HV2 in mitochondrial DNA has been performed as a tool for forensic identification, in addition to that of HV1. HV2 contains length heteroplasmy, which shows high variability within an individual or in maternal relatives. In this study, we used cloning analysis and PCR direct sequencing to compare, between mothers and their children, HV2 length heteroplasmic profiles in different tissues. For two mother-child pairs, different types of variant distribution were observed by cloning analysis. In pair 1, length heteroplasmic patterns in most tissues were similar (predominantly 9 and 10Cs variants), but different length heteroplasmic levels, with shifts in predominant genotype, were observed for some hairs in both mother and child. In pair 2, genotype distribution was similar for all tissues, with a predominant 8Cs genotype, but varying in the proportion of minor component. The proportion of one minor length variant (9Cs) in blood from the child was significantly higher than that from the mother, but the proportions of minor components (7 and/or 9Cs) in other tissue samples decreased from mother to child. Moreover, we could confirm that sequence types of PCR products were reflected by the distribution of length variants, which were observed especially in high proportion, in cloning analysis. Our results reveal variable changes in length heteroplasmic level in various tissues between generations. Variability between tissues, especially among hairs, within an individual would result in complicated differences in genotype distribution between maternal generations, and correlate with longer length of Cs for predominant variants.     

Lessons Learned from Autopsying an Unidentified Body with Iodine‐125 Seeds Implanted for Prostate Brachytherapy

We report here lessons learned from an autopsy case involving radioactive materials. We performed an autopsy of an unidentified mummified man with no available medical history whom from imaging findings we suspected had received radioactive seed implants for prostate brachytherapy. We returned the excised prostate and seeds to the body. A few days later, the body was identified by DNA matching and cremated. According to the man's medical record, he had undergone iodine‐125 seeds implantation for prostate cancer 11 months earlier. We should have removed the radioactive seeds from the body to prevent radiation exposure to the bereaved family and/or environmental pollution due to cremation. Surprisingly, one seed was found in the stored prostate specimen. Forensic experts should be cognizant of the risk of both radiation exposure in the autopsy room and environmental pollution. We must remain abreast of the latest advances in medicine.     

Potentiation of lethality and increase in body temperature by combined use of d-methamphetamine and morphine in mice

Lethality and change in body temperature in mice were examined after subcutaneous injection of d-methamphetamine and morphine alone or in combination. The LD50 values for methamphetamine and morphine were calculated to be 95 and 670 mg/kg body wt., respectively. When a non-lethal dose of morphine (300 mg/kg) was administered with various doses of methamphetamine, the LD50 for methamphetamine was reduced to 5 mg/kg, indicating a marked potentiation of toxicity by combined use of both drugs. Injection of 5 mg/kg of methamphetamine produced slight hyperthermia, while 300 mg/kg of morphine decreased the body temperature of mice. However, when both drugs were used concomitantly, a marked increase in body temperature was observed. Hyperthermia was also observed when the dose of morphine was reduced to 50 mg/kg. It is postulated that hyperthermia is probably one of the contributory factors in the potentiated toxicity by combined use of morphine and methamphetamine.     

Social Capital, Rehabilitation, Tradition: Support for Restorative Justice in Japan and Australia

This paper investigates the attitudes and beliefs that the public hold about criminal behaviour in Japanese and Australian society, with a view to uncovering sources of resistance to, and support for, restorative justice. The study draws on a survey of 1,544 respondents from Japan and 1,967 respondents from Australia. In both societies, restorative justice met with greater acceptance among those who were (1) strong in social capital, (2) believed in offender reintegration and rehabilitation, (3) saw benefits for victims in forgiveness, and (4) were advocates for victims?? voices being heard and amends made. The alternative ??just deserts?? and deterrence models for dealing with crime were grounded in attitudes of punitiveness and fear of moral decay, and reservations about the value of reintegrating and rehabilitating offenders. Like restorative justice supporters, ??just deserts?? and deterrence supporters expressed concern that victims?? voices be heard and amends made. Winning public support for competing institutional arrangements may depend on who does best in meeting expectations for meeting the needs of victims.     

The representative jury requirement: Jury representativeness and cross sectional participation from the beginning to the end of the jury selection process

The paper specifically addresses the many ways in which the facially neutral procedures actually fail to secure representative jury pools. Although the Sixth Amendment's fair cross‐section requirement forbids systematic discrimination in the creation of the jury venire and panel, it does not guarantee that the criminal jury will in fact reflect an accurate cross‐section of the community. As a result, not only does the Court fail to focus on nonlegally recognized screening mechanisms and factors such as exemptions, excuses, failure to followup jurors, etc., may affect jury representativeness, but also the Court never examined cross‐sectional representation at the entirety of the jury selection processes, except jury panels and final juries.

The first section of this paper presents a brief overview of the constitutional law impacting impartial juries, especially addressing the fair cross‐section doctrine that is the focus of contemporary jury selection procedures. In providing empirical and systematic comparisons of jury participation at each of the distinct jury selection stages encompassing a general population, jury wheels, jury qualified pools, jury eligibles, jury panels, and actual trial jurors, the second section of this paper makes critical analyses of the cumulative effects of screening mechanisms in jury selection. The paper assesses jury compositions by looking at demographic, socio‐economic, and ideological profiles of prospective jurors, illustrating that those jury profiles do not necessarily reflect cross‐sectional representation of the community population at comprehensive stages of the jury selection process. The analytical findings show that unless some deep seated reforms are made to eliminate cumulative effects of selection biases and correct representative imbalances of jury wheels, qualified pools, jury panels, and trial juries, historically underrepresented groups such as racial minorities, the poor, and part‐time employees will continue to be underrepresented on juries, negating the public's shared responsibility for the administration of justice in one of America's most heralded democratic institutions.     

A Dawn of Two-Party System in Japanese Politics?: The Emergence of the Democratic Party of Japan

The long rule of the Liberal Democratic Party (LDP) has finally ended, and the Democratic Party of Japan (DPJ) has captured government. This article is to examine why and how that has ended and what are the major factors which have contributed to the change of government. The inability of Japanese opposition parties to capture government has been discussed for a long time. The first part of this article is to examine the strength and weakness of major structural explanations which have been discussed in existing literature. Then, the article proposes a process-level analysis which takes a synergistic effect among institution, strategy and chance seriously. The second part of the article is a short history of the emergence of the Democratic Party of Japan (DPJ) as a ruling party. The history will start with a formation of a small political party in September 1996,and describe how a new single-member electoral system shaped the incentives of political actors, how they worked out political strategy under the new system, and how chance affect the success or failure of political strategy.