An exploration of the “Spinback” phenomenon

This paper examines nine cases of “spinback,” the flow back to laboratories of technical advantage from technology transfer activities. The benefits revealed in the cases are discussed and assessed for their value to the participating laboratories and the scientists involved. The objective of this study was to explore and test the feasibility of undertaking a comprehensive study of the value of spinback in order to encourage support for technology transfer activities by middle management in a laboratory. Spinback is defined as the phenomenon by which scientists’ and engineers’ participation in technology utilization activities results in a technically valuable flow back to their laboratories or organizations, thereby increasing or improving techical capability. It was anticipated that this exploration would provide evidence that the phenomenon does occur, with important consequences for the organization.     

Ribosomal ribonucleic acid (rRNA) gene typing for species identification.

Deoxyribonucleic acid (DNA) typing of ribosomal ribonucleic acid (rRNA) genes was performed with a polymerase chain reaction (PCR) assay for species identification. A variable region of the 28S ribosomal RNA gene was amplified with primers complementary to flanking sequences phylogenetically well conserved. The products of twelve animal DNAs (human, Japanese monkey, dog, cattle, pig, cat, rabbit, mouse, rat, chicken, frog, and fish) were separated by polyacrylamide gel electrophoresis, each revealing a few bands ranging from 150 to 100 base pairs. The band patterns obtained from each DNA sample differed in number and size, which indicates the applicability of the method to species identification. Samples containing either as little as 1 pg of DNA or degraded DNA of 0.2 to 0.5 kb in length were able to give detectable bands. Postmortem human tissue DNAs were tested as an example. They showed a pattern identical to the human control one, which was distinct from those of the other animals examined.     

Americans and their sexual partners

Andrew M. Greeley is a priest, sociologist, novelist, and journalist. His scholarly writings concentrate on religion and ethnicity. His other writings range from critiques of the Catholic church to best-selling novels.     

Symposium: Policy analysis for congress. Policy analysis for congress: Lengthening the time horizon

Policy analysis for the Congress is a timely subject. Several policy initiatives have emerged recently from the “first branch.” Others have demonstrated an independent capacity for analysis that is impressive by historical standards. This article introduces a practitioner symposium of three related works on policy analysis for Congress, with appropriate commentary by a distinguished veteran. It argues that because timing is crucial in the congressional policy process, anticipation and advance preparation are central to the effective use of analysis. The other articles illustrate the stages of advance preparation—of the policy analyst, the congressional environment, and the Member for voting—in the form of case studies.     

On the politics of traditional rulership

Conclusion We have in this paper tried to set out a framework which can facilitate analysis of the current and future situation of traditional rulership in contemporary African society. Our presentation of a proposed model for such an analysis is no doubt still in its elementary stages of formation. Much more work needs to be done. Nevertheless, our analysis has revealed certain salient elements and uncovered certain unresolved issues. If traditional rulers genuinely care about their own future they will have to devise techniques of legitimation which not only endear them to the government and the non-traditional elites, but also, and more importantly, to the mass of the people. True, the original role of the traditional rulers may be no more; still, it may be possible to salvage what is left.     

Threatening and otherwise inappropriate letters to members of the United States Congress

The authors examine the characteristics of threatening and otherwise inappropriate communications sent to members of the U.S. Congress by a sample of 86 subjects, 20 of whom threatened assassination. We quote excerpts from these letters and provide quantitative data on such variables as the volume, duration, form, and appearance of such communications; the enclosures; the subjects' perceived relationships to the recipients; the thematic content of the communications; and the messages and threats communicated. Comparisons between 43 subjects who pursued encounters with members of Congress and 43 who did not revealed 17 factors associated with such pursuit. In this population, threateners were significantly less likely to pursue an encounter than inappropriate letter writers who did not threaten, regardless of the type of threat or the harm threatened. Inappropriate letters to members of Congress are compared with those directed to Hollywood celebrities. Mentally disordered persons writing to public figures often mention and sometimes threaten public figures other than those to whom the letters are addressed, which raises important issues regarding notification of endangered third parties and the sharing of information among protective agencies.     

Back to the future of ‘one logic or two’?: forward to the past of ‘anarchy versus racist hierarchy’?

This article argues that while the attempt by Alex Callinicos to construct a non-reductionist approach for theorizing the international is brave, it falls short of the target due, in part, to the failure to look in the right place for a non-reductionist Marxism. In taking us ‘back to the future’ of the debate on ‘one logic or two?’ (‘economics’ or ‘geopolitics and economics’), the quest can at best result only in the construction of a thin materialist reductionism. This article develops an approach that takes norms and (racist) identity seriously—one that simultaneously reconfigures our conception of the international as a hierarchy rather than as a pure anarchy, thereby prompting a reconsideration of Callinicos's commitment to a neorealist anarchic conception of the international. This article closes by suggesting that the Eurocentrism of much Marxist International Relations scholarship obscures the role of non-Western resistance in the making of global politics.     

The use of phosphate buffered saline for the recovery of cells and spermatozoa from swabs.

In the forensic science laboratory, the recovery of spermatozoa from vaginal swabs, or vaginal cells from penile swabs, can help determine if sexual intercourse may have taken place. There are several methods used to recover spermatozoa and cells from the swabs before visualisation on a microscope slide and most of these methods use water. Phosphate buffered saline (PBS) is a non-toxic solution used in many biological laboratories. Unlike water, PBS prevents cells rupturing or shrivelling up due to osmosis. This study demonstrates that PBS can be used for the extraction of spermatozoa and cells from swabs and that PBS does not affect subsequent DNA profiling.     

Judicial Procedures as Instruments of Political Control: Congress's Strategic Use of Citizen Suits

Citizen suit provisions, which give proregulatory interests access to the federal courts, can be used by Congress to increase the courts' role in regulatory policy. I analyze 284 environmental regulation bills reported favorably out of committee and show that committee support for citizen suits is a function of the committee's policy goals and the political context in which the bills are generated. These findings indicate that Congress deliberately uses judicial procedures as instruments of political control and that scholars examining judicial policymaking must include legislative goals in the list of explanatory factors.     

柔嫩艾美球虫杨凌株表面抗原SAG10基因的克隆与原核表达

从柔嫩艾美球虫第二代裂殖子总RNA中扩增EtSAG10基因,与pGEM-T Easy载体连接后转化大肠杆菌(E.coli)DH5α,筛选阳性克隆,扩增不含N端信号肽的编码序列,分别插入表达载体pET-32a( )和pMAL-c2X,转化至E.coliRosetta,以IPTG诱导表达。结果表明,pET-32a( )-EtSAG10在E.coliRosetta中的表达产物约占菌体总蛋白的43%,融合蛋白分子质量约为47 ku,以包涵体形式存在;而pMAL-c2X-EtSAG10在E.coliRosetta中表达的重组蛋白为可溶性,表达产物约占菌体总蛋白的35%,融合蛋白分子质量约为69 ku。以表达的可溶性EtSAG10重组蛋白100μg/只肌肉注射免疫雏鸡,攻虫后以盲肠病变计分、盲肠卵囊数(OPG)、相对增重率和抗球虫指数(ACI)评价,免疫组相对盲肠卵囊产量为47.7%,抗球虫指数由86.79提高至152.13。提示,重组表达的EtSAG10可诱导雏鸡产生一定的抗球虫免疫保护。