Analysis of nitrite in adulterated urine samples by capillary electrophoresis

A simple method for analyzing nitrite in urine has been developed to confirm and quantify the amount of nitrite in potentially adulterated urine samples. The method involved separation of nitrite by capillary electrophoresis and direct UV detection at 214 nm. Separation was performed using a bare fused silica capillary and a 25 mM phosphate run buffer at a pH of 7.5. Sample preparation consisted of diluting the urine samples 1:20 with run buffer and internal standard, and centrifuging for 5 min at 2500 rpm. The sample was hydrodynamically injected, then separated using -25 kV with the column maintained at 35 degrees C. The method had upper and lower limits of linearity of 1500 and 80 microg/mL nitrite, respectively, and a limit of detection of 20 microg/mL. The method was evaluated using the National Committee for Clinical Laboratory Standards (NCCLS) protocol (Document EP10-A2), and validated using controls, standards, and authentic urine samples. Ten anions, ClO-, CrO4(-2), NO3-, HCO3-, I-, CH3COO-, F-, SO4-, S2O8(-2), and Cl-, were tested for potential interference with the assay. Interferences with quantitation were noted for only CrO4(-2) and S2O8(-2). High concentrations of Cl- interfered with the chromatography. The method had acceptable accuracy, precision, and specificity.     

Adolescent women's developing identity: A study of self-definition in the context of family relationships

This study generated adolescent women's perception of their identity in relation to family members spanning three generations and related these perceived relationships to their sex-role orientation. Subjects were 20 firstborn university women from intact families. The methodology used multiple sources of information, including open-ended interviewing procedures, rating scales, and standard research measures of sex-role identity. Significantly more constructs empirically differentiated family by generation than by sex. Congruence of young women with both the parent and grandparent generation, relative number of masculine stereotypes produced, and personality traits of males and females were significantly influenced by the presence of a brother in the sibling generation. There was no relationship between family constellation and sex-role orientation. Feminine women were significantly more congruent with other females in their family than androgynous women. There was a linear trend for androgynous women to be increasingly individuated across the generations.Received her Ph.D. from Yale University. Research interests include observation of children and families in natural settings, longitudinal research with at-risk infants, and rural consultation.Received her Ph.D. from the University of Minnesota. Research interests include development of social competence in family, school, and community environment.Received his Ph.D. from Yale University. Research interests include the socialization of values, professional development, and studies of the family in religious and ethnic community contexts.     

Positive Resolution of Childhood Sexual Abuse Experiences: The Role of Coping,Benefit-Finding and Meaning-Making

Resolution of the trauma of childhood sexual abuse (CSA), and the current adjustment of 60 adult female CSA survivors were explored through qualitative and quantitative analyses of their coping strategies, perceived benefits, and meaning-making attempts. While the majority of the women (87%) perceived at least some benefit resulting from coping with the CSA experience, many (29%) found it impossible to make any meaning of their trauma. Specific benefits that were associated with various aspects of positive adjustment (marital satisfaction, better physical health, less isolation) included improved relationships with others, religious or spiritual growth, and improved parenting skills. Some perceived benefits were actually associated with a negative outcome. Increased knowledge of sexual abuse was associated with more isolation and lower marital satisfaction. When positive meaning could be derived from the coping process, the women reported less isolation. Avoidant coping was strongly associated with more depressive symptoms and poorer resolution of abuse issues. Results highlight the importance of considering coping strategies and cognitive restructuring efforts in designing therapeutic interventions with this population. Portions of this paper were presented at the annual meeting of the Midwest Psychological Association, May 2004, Chicago, IL.     

Distributive Justice Norms and Family Work: What Is Perceived as Ideal,What Is Applied,and What Predicts Perceived Fairness?

Members of married couples rated how ideal communal, exchange, and equality rules were for their marriages. They also reported on whether they and their spouses followed each norm in general in their marriages and in the domains of housework and child care. Both men and women considered a communal norm to be more ideal than the other norms and reported that they and their spouse followed a communal norm to a greater extent than an exchange or equality rule, both in general and in the domains of housework and child care. In addition, links between reports of actual division of labor in each domain, as well as reports of norm use, and perceived fairness of the division of family work were examined. Greater inequalities in the division of labor were linked to decreased perceptions of fairness. Controlling for reports of division of labor, women's reports of the self and of the spouse following a communal norm were linked with increased perceptions of fairness as were women's reports of the spouse following an equality rule. In contrast, and again after controlling for reports of division of labor, women's reports of the self following an exchange rule and men's reports of the self and of the spouse following an exchange rule were associated with greater perceived unfairness of the division of family work.