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231.
Bezerra de Menezes LM Volpato MC Rosalen PL Cury JA 《Forensic science international》2003,137(2-3):209-214
The use of bone as a biomarker for chronic and acute exposure to fluoride salts has been suggested, but there are no data published about its use to evaluate lethal intoxication. One hundred and sixty rats were divided into eight groups that received a single oral intubation dose from 0 (control) to 90 mg F/kg as NaF. The animals' time of death was recorded and their femurs were removed for fluoride analysis. Acid-soluble fluoride was determined in the whole bone and on the surface (periosteal), using an ion specific electrode. The data showed a statistically significant relationship between fluoride dose and the number of deaths (P<0.0001). A statistically significant relationship was also found between fluoride dose and fluoride concentration ([F]) in either the whole femur (P<0.0017), on the surface (P<0.0001) or for the ratio periosteal [F]/whole [F] (P<0.0001). However, the [F] on the femur surface was more closely correlated with mortality than that in the whole bone, showing statistically significant differences among the lethal doses and control (P<0.05). The data suggest that the ratio [F] periosteal bone/[F] whole bone, is a biomarker for acute fluoride toxicity. 相似文献
232.
Sánchez-Diz P Gusmão L Beleza S Benítez-Páez A Castro A García O Solla LP Geada H Martín P Martínez-Jarreta B de Fátima Pinheiro M Raimondi E Silva de la Fuente SM Vide MC Whittle MR Zarrabeitia MT Carracedo A Amorim A;GEP-ISFG 《Forensic science international》2003,135(2):158-162
A collaborative exercise was carried out by the Spanish and Portuguese ISFG Working Group (GEP-ISFG) in order to evaluate the performance of two Y-chromosome STR PCR tetraplexes, which include the loci DYS461, GATA C4, DYS437 and DYS438 (GEPY I), and DYS460, GATA A10, GATA H4 and DYS439 (GEPY II). The participating laboratories were asked to type three samples for the eight markers, using a specific amplification protocol. In addition, two control samples, with known haplotypes, were provided. The results obtained by the 13 different participating laboratories were identical, except for two laboratories that failed to type correctly the same two samples for GATA C4. By sequence analyses, two different GATA C4 allele structures were found. One control sample (allele 21) and two questioned samples (allele 22, correctly typed by all the laboratories, and allele 25) presented the following repeat structure: (TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)n, but different from the one found for allele 26 in one sample included in this exercise, as well as in the second control sample (allele 23), namely (TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)2(TGTA)2(TCTA)n. The collaborative exercise results proved that both Y-tetraplexes produce good amplification results, with the advantage of being efficiently typed using different separation and detection methodologies. However, since GATA C4 repeat presents a complex structure, with alleles differing in sequence structure, efficient denaturing conditions should be followed in order to avoid typing errors due to sizing problems. 相似文献
233.
Calacal GC De Ungria MC Delfin FC Lara MC Magtanong DL Fortun R 《The American journal of forensic medicine and pathology》2003,24(2):148-152
We describe here our collaborative efforts in identifying 2 fatalities of a fire disaster by using a variety of identification techniques. Postmortem findings in both cases were reinforced using Short Tandem Repeat (STR) DNA technology to establish with a high degree of certainty the identities of 2 child victims. STR markers used in the present study include HUMAMEL, HUMCSFIPO, HUMTHO1, HUMvWA, HUMFES/FPS, HUMF13A01, HUMFOLP23, D8S3O6, HUMFGA, and HUMTPOX. Unambiguous identification was made possible through matching DNA profiles generated from skeletal remains with those from umbilical tissues. These tissues were kept by their mothers in accordance with a Philippine tradition and were submitted for DNA analysis. Of the DNA profiles generated from exhumed bone samples of 21 child victims, comparison with the genetic profiles of children A and B obtained from umbilical tissues showed consistent DNA matches with remains 1756 and 1758, respectively. 相似文献
234.
Episodes of husband-to-wife violence in which the husband consumed alcohol were compared with episodes of husband-to-wife violence, reported by the same individuals, in which the husband was not drinking. Among this community sample of newlyweds, wife reports, but not husband reports, indicated that violent episodes in which the husband was drinking included more acts of violence and were more likely to involve severe violence. Both wife and husband reports indicated that wives were more likely to be physically aggressive in husband drinking episodes compared to sober episodes. However, whereas wives reported that their aggressive behavior was a response to husband aggression, husbands reported that wives were more likely to initiate violence in these episodes. Violent episodes that include alcohol may be more severe and more mutually violent than sober episodes. Discrepancies between husband and wife reports may reflect differences in recall or self-serving biases. 相似文献
235.
Sudden death is the most common manifestation of myocardial sarcoidosis, which is often clinically silent. The disease is rarely encountered in forensic medicine, but the diagnosis is important because it may change the interpretation of the manner of death and thus have insurance implications. Six cases of myocardial sarcoidosis diagnosed at autopsy are reported, and the significance of the findings is discussed. 相似文献
236.
Maria Nedeva Luke Georghiou Peter Halfpenny 《The Journal of Technology Transfer》1999,24(2-3):139-147
This paper examines the role of industry in the support of academic infrastructure, in particular university research equipment. Although the United Kingdom provides the framework for discussion the described situation should be a familiar one in most countries. The argument is constructed around the perceptions, opinions and positions of universities, government and industry. Drawing on results from a survey of academic departments the equipment situation at UK universities is outlined. Following that the position of the Government attempting to attract industrial support for university research equipment is discussed. And finally, industry's views of where the demarcation between public and private responsibility lies are presented. 相似文献
237.
Maria Maley 《Australian Journal of Public Administration》2002,61(1):103-107
The role of the ministerial office was one of the key issues investigated by the Royal Commission on Australian Gvoernment Administration (RCAGA) in 1976. At the time the ministerial office was undergoing new and controversial developments. In the 25 years since the Royal Commission the new ministerial office has become a permanent and accepted part of our machinery of government. This paper reviews RCAGA’s analysis of ministerial advisers and uses research on the Keating advisers in 1995–96 to track how the institution of the ministerial office has developed since the time of the Royal Commission. 相似文献
238.
239.
Maria Escobar-Lemmon 《American journal of political science》2003,47(4):683-697
A worldwide trend of decentralizing political power, fiscal authority, and the responsibility for providing services to subnational governments has been especially evident in Latin America over the past decade. This article focuses on variation in intracountry support for decentralization, using data on bill initiation from Colombia and Venezuela to determine the domestic sources of this policy. While the data support prior findings that decentralization is supported by parties whose future electoral prospects are better at the subnational level, the analysis also shows that citizen's trust in government and greater wealth affect a deputies' support for decentralization . 相似文献
240.
Maria Laura Seguiti 《Public Budgeting & Finance》2003,23(2):96-117
What is the role of the European Union (EU) budget in the upcoming EU enlargement? This article attempts to answer this question by focusing on the EU budgetary system before and after the enlargement. The analysis of the EU budget helps us understand the political and economic philosophy as well as the practical achievements of the European construction. The upcoming enlargement urges the elaboration of a new vision of Europe, its missions and democratic institutions, and the power sharing between the EU and its member states, with a consequent major reform of EU fiscal and budgetary discipline. 相似文献