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A simple tandem repeat (STR) PCR-based typing system developed for the genetic individualization of domestic cat samples has been used to generate a population genetic database of domestic cat breeds. A panel of 10 tetranucleotide STR loci and a gender-identifying sequence tagged site (STS) were co-amplified in genomic DNA of 1043 individuals representing 38 cat breeds. The STR panel exhibits relatively high heterozygosity in cat breeds, with an average 10-locus heterozygosity of 0.71, which represents an average of 38 breed-specific heterozygosities for the 10-member panel. When the entire set of breed individuals was analyzed as a single population, a heterozygosity of 0.87 was observed. Heterozygosities obtained for the 10 loci range from 0.72 to 0.96. The power for genetic individualization of domestic cat samples of the multiplex is high, with a probability of match (p(m)) of 6.2E-14, using a conservative θ = 0.05. 相似文献
178.
Lisa Dillon Marilyn Amorevieta-Gentil Marianne Caron Cynthia Lewis Angélique Guay-Giroux Bertrand Desjardins 《The History of the Family》2018,23(1):20-53
The Programme de recherche en démographie historique (Historical Demography Research Programme) (PRDH), founded in 1966 and based at the Département de Démographie of the Université de Montréal, has since its inception featured a central project, a family reconstitution database of Quebec’s Catholic population from 1621 to 1799 named the Registre de la population du Québec ancien (Population Register of Historic Quebec) (RPQA). This article, which marks the fiftieth anniversary of the project, explores the development of the RPQA over the five decades in the context of similar international databases, explains the current state of the database as well as our record linkage methodology, describes an important collaboration now underway to build a larger Quebec historical data infrastructure, outlines new and renewed international collaborations, and summarizes research conducted using these data as well as future research possibilities. The particular geographic context, historical development and manageable colonial population size of Quebec favoured family reconstitution of the whole colony from the beginning of the project. Today, the RPQA comprises 438,193 individual biographies and 74,000 family files encompassing up to nine generations. To reconstitute families, we must identify and incorporate into the database all demographic events, including those whose existence can only be inferred through other sources. Future efforts to link nineteenth-century parish acts will need to deal with large case counts, mixed Catholic–Protestant marriages, and increased geographic and social mobility. The integration of complementary data will provide information on household co-residence, occupations, help track the destinies of mixed-religion persons and persons outside nuclear families and provide additional points of observation. 相似文献
179.
Marilyn Silberfein Shelly Kessler 《Studies in Comparative International Development (SCID)》1988,23(1):85-101
The distribution of services, personnel, and infrastructure in rural development projects has not usually been subject to
a thorough scrutiny. Locational decisions have been arbitrary, without reference to the needs and mobility of the rural population.
This study advocates one particular revision of past policies: the incorporation of small towns into programs designed to
improve agricultural production and economic opportunity in rural regions. When such towns already function as a center for
political activity, marketing, employment, and service provision, a development project can reinforce these linkages in ways
that enhance productivity. A case study is used to illustrate the efficacy of this proposal: the town of Panguma, Kenema District,
Sierra Leone and its role in development programs. Panguma has several features that make it an ideal focal point, but it
has not been incorporated into regional projects in any systematic way. This town and its hinterland provide an illustration
of how rural-urban interaction at a local scale can further the achievement of rural development goals.
C.A.R.E. 相似文献
180.
Robert J. Levine 《The Journal of law, medicine & ethics》1988,16(3-4):174-182
"It is a fact of life that human beings find it difficult to maintain a consistent, self-conscious appreciation of the extent to which uncertainty accompanies them on their daily rounds and to integrate that uncertainty with whatever certainties inform their conduct. Physicians are not exempt from this human proclivity." Jay Katz1 相似文献