Confirmation by LC–MS of drugs in oral fluid obtained from roadside testing

The aim of this study was to assess the effectiveness of two current on-site oral fluid (OF) drug detection devices (OraLab and Dräger), as part of the Spanish participation in the Roadside Testing Assessment Project (ROSITA Project). The study was done in collaboration with the Spanish Traffic Police, in Galicia (NW Spain), during 2004 and 2005. A total of 468 drivers selected at the police controls agreed to participate through informed consent. In addition, saliva samples were collected and sent to the laboratory to confirm the on-site results. For this purpose, two different analytical liquid chromatography–mass spectrometry (LC–MS) methods were used to detect 11 drugs or metabolites in a 300 μL sample. Simultaneous analysis of morphine, 6-acetylmorphine, amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB, cocaine and benzoylecgonine was carried out using 100 μL of oral fluid, after an automated solid phase extraction. A different LC–MS method was performed to detect Δ⁹-THC in 200 μL of oral fluid using liquid–liquid extraction with hexane at pH 6. Both methods were fully validated, including linearity (1–250 ng/mL, 2–250 ng/mL) recovery (>50%), within-day and between-day precision (CV < 15%), accuracy (mean relative error < 15%), limit of detection (0.5 and 1 ng/mL), quantitation (1 and 2 ng/mL) and matrix effect. All of the positive cases and a random selection of 30% of the negatives were analyzed for confirmation analysis. Good results (sensitivity, specificity, accuracy, positive predictive value and negative predictive value > 90%) were obtained for cocaine and opiates by OraLab, and for cocaine by Dräger. However, the results for the other compounds could be improved for both detection devices. Differences in the ease of use and in the interpretation mode (visual or instrumental) were observed.     

Defining and classifying serial murder in the context of perpetrator motivation

The last several decades have seen increased research attention to the subject of serial murder. Unfortunately, an empirical understanding of serial homicide continues to be impeded by difficulties in establishing a working definition for serial murder. Current definitions for serial homicide tend to focus on frequency and timing of homicide behaviors, and thus leave some confusion as to which groups of individuals should be included under the heading of serial murders. This article suggests alterations to the definition of serial murder, which focus not only on frequency and timing of homicide behaviors, but also on the motivation and context of those behaviors.     

Voting as a habit in new democracies – Evidence from Poland

Among the wide range of theories explaining why some people vote and others do not, one is recently gaining particular popularity. This is the theory of voting as a habit (e.g. Plutzer, 2002; Franklin, 2004; Hooghe, 2004). The empirical evidence supporting this theory covers only Western democracies, so the following question might be asked: is this pattern universal? In the case of old democracies, voting is a habit acquired gradually in a process which starts at the moment of the very first election one can cast the ballot. In new democracies the situation is different, as we can pinpoint the starting moment (first democratic election), which is the same for different voters and thus different age cohorts. In this paper we investigate voting as a habit in new democracies, using data from the Polish National Election Study. We find that voting in Poland has some habitual aspect; repeated voting brings about a (sort of) habit, which has an intrinsic, irreducible effect on voter turnout. We also find that habit of voting is formed likewise in all age cohorts.     

‘you like to mix things up on purpose …? hoy,what are you trying to prove?’: representations of recent (hi)stories in Jessica Hagedorn's <Emphasis Type="Italic">The Gangster of Love</Emphasis>

This paper examines how The Gangster of Love (1996), the second novel by Filipino American artist and writer Jessica Hagedorn, dismantles ready-made assumptions about the construction of minority and mainstream cultures. Spanning the period from the 1970s to the early 1990s, Gangster depicts the life of Rocky Rivera, a Filipina American young artist. As it portrays Rocky's family and friends, the novel examines the drastic re-articulation of the US’s self-image brought about by Filipino Americans and other groups marginalized as minorities by virtue of their ethnic, socio-cultural, and/or sexual identities. The narrative challenges dominant notions of who and what makes history, juxtaposing historical references with fictionalizations of real episodes and completely fictional incidents, and making a constant use of parody, irony, jokes and clichés. This paper studies Rocky's reworks of her multiple ethnic and socio-cultural allegiances in connection with her passion for music and popular culture, placing these reworks in the context of the Philippines’ colonial and neo-colonial background and multi-cultural US history. It also considers succinctly how Gangster's postcolonial dimensions intersect with a feminist and postmodern consciousness in a gendered strategy of socio-cultural resistance and critique.     

Fast LC-MS/MS method for the determination of amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB and PMA in urine

A fast method was designed for the simultaneous determination of amphetamine (A), methamphetamine (MA), PMA, MDA, MDMA, MDEA and MBDB in urine. The drugs were analysed by LC (ESI)-MS/MS, after a simple liquid-liquid extraction in the presence of the deuterated analogues. Reverse phase separation on an Atlantis dC18 Intelligent Speed column was achieved in less than 4 min under gradient conditions, and the total run time was 8 min. The method was fully validated, including linearity (1-1000 ng/mL for A, MDMA, MDEA and MBDB; 2-1000 ng/mL for MDA and PMA; 1-200 ng/mL for MA; r2>0.99 for all compounds), recovery (>80%), within-day and between-day precision and accuracy (CV and MRE<12.7% for intermediate level and ULOQ, and <17.2% for LLOQ), limit of detection (0.2 ng/mL for MDMA, MDEA and MBDB; 0.5 ng/mL for A, MA and PMA; 1 ng/mL for MDA) and quantitation (1 ng/mL for A, MA, MDMA, MDEA and MBDB; 2 ng/mL for MDA and PMA) and relative ion intensities. No matrix effect was observed. The procedure proved to be sensitive, specific and rapid, and was applied to real forensic cases.     

Confirmation by LC-MS of drugs in oral fluid obtained from roadside testing

The aim of this study was to assess the effectiveness of two current on-site oral fluid (OF) drug detection devices (OraLab and Dr?ger), as part of the Spanish participation in the Roadside Testing Assessment Project (ROSITA Project). The study was done in collaboration with the Spanish Traffic Police, in Galicia (NW Spain), during 2004 and 2005. A total of 468 drivers selected at the police controls agreed to participate through informed consent. In addition, saliva samples were collected and sent to the laboratory to confirm the on-site results. For this purpose, two different analytical liquid chromatography-mass spectrometry (LC-MS) methods were used to detect 11 drugs or metabolites in a 300 microL sample. Simultaneous analysis of morphine, 6-acetylmorphine, amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB, cocaine and benzoylecgonine was carried out using 100 microL of oral fluid, after an automated solid phase extraction. A different LC-MS method was performed to detect Delta(9)-THC in 200 microL of oral fluid using liquid-liquid extraction with hexane at pH 6. Both methods were fully validated, including linearity (1-250 ng/mL, 2-250 ng/mL) recovery (>50%), within-day and between-day precision (CV<15%), accuracy (mean relative error<15%), limit of detection (0.5 and 1 ng/mL), quantitation (1 and 2 ng/mL) and matrix effect. All of the positive cases and a random selection of 30% of the negatives were analyzed for confirmation analysis. Good results (sensitivity, specificity, accuracy, positive predictive value and negative predictive value>90%) were obtained for cocaine and opiates by OraLab, and for cocaine by Dr?ger. However, the results for the other compounds could be improved for both detection devices. Differences in the ease of use and in the interpretation mode (visual or instrumental) were observed.     

Toward the Implementation of Intersectionality in the European Multilevel Legal Praxis: B. S. v. Spain

This article identifies the factors that contribute to the successful implementation of intersectionality in European multilevel legal praxis through the analysis of the case B.S. v. Spain. Combining critical legal analysis of the main judicial documents with qualitative methodology from political science based on in‐depth interviews with key actors involved in the case, we uncover the obstacles and opportunities existing at the national and supra‐national levels for the implementation of intersectionality. We decipher the factors contributing to the exceptional success of this case through the conjoint analysis of macro, meso, and micro levels. Our analysis shows that a combination of the legal provisions, paradigms and structures, the roles of the different actors involved, and the applicant's subjective position made it possible to put intersectionality in practice. This study provides novel empirical evidence that contributes to advancing the theoretical debate about intersectionality implementation in the multilevel European context.     

Electoral Reform as an Engine of Party System Change in Italy

Abstract

After more than forty years of proportional representation, in 1991 Italy embarked on a period of electoral law revision. The ongoing debate about how to engineer an electoral system capable of producing the preferred political outcomes stands out against the stasis in constitutional reform, most recently demonstrated in the rejection by popular referendum of Matteo Renzi’s package of reforms. The extent to which the different electoral reforms have had an impact on Italian politics, especially following the 2005 electoral legislation, can be evaluated by analysing the changing Italian party system over the past decade and beyond in terms of its morphology, dynamics, and party/parliamentary group switching. The 2005 electoral reform has had a clear effect on Italian politics and on the party system, but that effect is unlikely to endure given the highly controversial new electoral law that came into force in 2017.     

Parental-leave policy for male lawyers in Helsinki and Montreal: cultural and professional barriers to male lawyers’ use of paternity and parental leaves

Family policies in Finland and French Canada (Québec) include fathers’ rights to paternity and parental leaves, which have resulted in more fathers using parental leave. Yet this policy has a limited outreach to male-dominated professions, including the legal profession. In this article, we examine attitudes to paternity and parental leaves among male lawyers and the motives behind their decisions to use or not use them. We approach the issue from the perspective of the legal profession’s professional ethos, which impacts lawyers’ attitudes and practices regarding work-life balance. In our analysis, we draw on 20 Finnish and 18 Quebecois interviews with current and former male lawyers from private law practices in two urban civil-law contexts: Helsinki, Finland and Montreal, Canada. The findings indicate that, in traditionally male-dominated professions, it is not enough to provide men with a statutory right to paternity and parental leave. There is also a need for organisational solutions and peer encouragement in the work environment so that men feel comfortable taking leave.