The 2000-2001 GEP-ISFG Collaborative Exercise on mtDNA: assessing the cause of unsuccessful mtDNA PCR amplification of hair shaft samples

We report the results of Spanish and Portuguese working group (GEP) of International Society of Forensic Genetics (ISFG) Collaborative Exercise 2001-2002 on mitochondrial DNA (mtDNA) analysis. 64 laboratories from Spain, Portugal and several Latin-American countries participated in this quality control exercise. Five samples were sent to the participating laboratories, four blood stains (M1-M4) and a sample (M5) consisting of two hair shaft fragments. M4 was non-human (Felis catus) in origin; therefore, the capacity of the labs to identify the biological source of this sample was an integral part of the exercise. Some labs detected the non-human origin of M4 by carrying out immuno-diffussion techniques using antihuman serum, whereas others identified the specific animal origin by testing the sample against a set of animal antibodies or by means of the analysis of mtDNA regions (Cyt-b, 12S, and 16S genes). The results of the other three human blood stains (M1-M3) improved in relation to the last Collaborative Exercises but those related to hairs yielded a low rate of success which clearly contrasts with previous results. As a consequence of this, some labs performed additional analysis showing that the origin of this low efficiency was not the presence of inhibitors, but the low quantity of DNA present in these specific hair samples and the degradation.As a general conclusion the results emphasize the need of external proficiency testing as part of the accreditation procedure for the labs performing mtDNA analysis in forensic casework.     

Genetic Identification of Communist Crimes’ Victims (1944–1956) Based on the Analysis of One of Many Mass Graves Discovered on the Powazki Military Cemetery in Warsaw,Poland

As the result of the communist terror in Poland, during years 1944–1956 more than 50,000 people died. Their bodies were buried secretly, and most places are still unknown. The research presents the results of identification of people buried in one of many mass graves, which were found at the cemetery Pow?zki Military in Warsaw, Poland. Exhumation revealed the remains of eight people, among which seven were identified genetically. Well‐preserved molars were used for the study. Reference material was collected from the closest living relatives. In one case, an exhumation of victim's parents had to be performed. DNA from swabs was extracted with a PrepFiler^® BTA Forensic DNA Extraction Kit and organic method. Autosomal, Y‐STR amplification, and mtDNA sequencing were performed. The biostatistical calculations resulted in LR values from 1608 to 928 × 10¹⁸. So far, remains of more than 50 victims were identified.     

Parents’ Self-efficacy Beliefs and Their Children’s Psychosocial Adaptation During Adolescence

Research has shown that parents’ perceived parental self-efficacy (PSE) plays a pivotal role in promoting their children’s successful adjustment. In this study, we further explored this issue by comparing psychosocial adaptation in children of parents with high and low PSE during adolescence. One hundred and thirty Italian teenagers (55 males and 75 females) and one of their parents (101 mothers and 29 fathers) participated in the research. Data were collected at T1 (adolescents’ mean age = 13.6) and T2 (mean age = 17.5). Parents reported their PSE at T1. At T1 and T2, adolescents reported their perceived academic self-efficacy, aggressive and violent conducts, well-being, and perceived quality of their relationships with parents. At T2, they were also administered questions by using Experience Sampling Method to assess their quality of experience in daily life. As hypothesized, adolescents with high PSE parents reported higher competence, freedom and well-being in learning activities as well as in family and peer interactions. They also reported fewer problematic aspects and more daily opportunities for optimal experience. Findings pointed to the stability of adolescents’ psychosocial adaptation and highlighted possible directions in future research.     

Correlates of Identity Configurations: Three Studies with Adolescent and Emerging Adult Cohorts

Adolescence and emerging adulthood are two core developmental periods in which individuals can develop a meaningful identity across domains. However, there is a lack of studies exploring correlates of different identity configurations. The purpose of this article was to fill this gap in examining correlates of configurations characterized by identity stability or instability in both ideological and relational domains or identity stability in one domain and instability in the other domain. Three studies were presented. In the first study, we investigated links between identity configurations and internalizing problem behaviors in early and middle adolescents (N=1,891; M (age) =14; 55% female); in the second study, we focused on associations between identity configurations and identity functions in late adolescents and early emerging adults (N=1,085; M (age) =19; 63% female); in the third study, we investigated relationships between identity configurations, sense of coherence, and basic psychological need satisfaction in emerging adults (N=489; M (age) =21; 71% female). Overall, findings highlighted that participants experiencing a condition of identity stability in both domains reported a better profile than their peers displaying a condition of instability in both realms. Further, individuals with identity stability only in one domain reported intermediate scores and the effect provided by each domain varied according to the correlate examined and the age group taken into account. Implications of these findings are discussed.     

“One,none, and a hundred thousand” recipes for a robust response to turbulence

Turbulence appears to be a “new normal” in current societies, and public organizations need to learn how to react and adapt to it. Scholars agree on the need for robust actions to respond to turbulence. Through a qualitative comparative analysis performed on the vaccination campaigns of all 20 Italian regions, our paper explores whether different and alternative models of robustness may exist to cope with turbulence. Results shed light on three alternative models featuring static, hybrid, and dynamic robustness. They also highlight that robust governance appears to involve a capacity to learn, and to employ this learning as circumstances demand.     

Evaluation of the placenta in a stillborn fetus to estimate the time of death

The authors present a case of a presumably unknown pregnancy, in which the newborn was found dead in the bathroom. The child was considered stillborn and the death due to natural causes. The literature on estimation of the time of fetal death is reviewed. It has been reported that certain placental histological changes are useful in predicting the time of death in stillborn fetuses, but little has been published about their potential role. Furthermore, parameters evaluated by literature in examining the placenta for determining time of fetal demise in cases of stillbirth are not uniform in the few studies that have been published. We emphasize that any attempt to estimate the time of fetal death without an adequate knowledge of placental morphological changes is futile and forensically unjustifiable, and that a comprehensive placental, external, and histological examination of the fetus is still the method of choice to gain a reliable forensic answer.