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Current procedures for human DNA quantitation reach their limit at 150 pg DNA, which is above the limit of the PCR profiling range using Profiler-Plus (Applied Biosystems, CA). This study tested the potential for the use of primate specific Alu sequences in forensic science for the sensitive detection and quantitaion of DNA. A fluorescently labelled primer pair was designed enabling high efficiency amplification of the core Alu sequence within primate DNA. Quantitation was performed by measurement of fluorescence intensity and comparison to a series of standard template DNA amounts via the construction of a standard curve. The new Alu-based quantitation protocol developed has shown its feasibility in more sensitively quantitating (100-2.5 pg) unknown amounts of human DNA for forensic use. The method is compatible with the use and throughput of current forensic procedures. 相似文献
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The purpose of this study was to examine age and gender differences in peer conflict, particularly in regards to conflict issues and resolution strategies reported by children and adolescents. Students from grades 4 and 8 (60 boys, 60 girls) were asked interview questions and given 3 hypothetical scenarios to respond to. Teacher and self-reports were also gathered for each student to assess their level of adaptive ability. The results showed that adolescents tended to report higher rates of conflict and endorsed more cooperative strategies than 4th graders, who endorsed more aggressive tactics. Female students reported having more relational issues and used more conflict-mitigating strategies, while boys reported having more conflicts related to status/dominance. Finally, links were also found between effective resolution strategies and social ability. The implications and limitations of this study are discussed.M. A. Noakes received her Master's degree from the Department of Educational Psychology at the University of Alberta, Canada. Her current research interests include assessment of at-risk youth, relapse prevention programs for young offenders, and social emotional functioning of children and adolescents.C. M. Rinaldi her doctorate from the Department of Educational and Counselling Psychology at McGill University, Canada. Her current research interests include applied developmental psychology, social emotional functioning of children and adolescents, and parent-child relations. 相似文献
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Behavior in social-dilemma (mixed-motive) situations has been of great interest to economists, psychologists, and negotiation scholars. In this study, we used a threshold social-dilemma game to examine factors that have not yet been investigated and that may have an impact on behavior in these settings: gender and group identity. We found that, for women, interacting with members of a naturally occurring group increased coordination and efficiency, while for men, interacting with members of a naturally occurring group decreased coordination and efficiency. Psychological literature on gender differences and group interdependence explains these differences. We conclude by discussing the implications of these results for gender differences in negotiation behavior. 相似文献
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This article makes two major points in regard to expert psychological testimony on eyewitness identification. First, the attention devoted by psychologists to eyewitness identification issues is far out of proportion to the incidence of trials involving eyewitness identifications of criminal defendants; furthermore, the often-expressed concern over wrongful convictions is probably misplaced. Second, the experimental methods used in studies of eyewitness performance are fundamentally unsuited for drawing conclusions about actual witnesses. Hence, there is not an adequate scientific foundation for expert psychological testimony on eyewitness identification. Archival research is perhaps the most promising approach to the study of the criminal justice system. 相似文献
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