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Aedrianee Reeza Alwi MSc Naji Arafat Mahat PhD Faezah Mohd Salleh PhD Seri Mirianti Ishar PhD Mohammad Rahim Kamaluddin PhD Mohd Radzniwan A. Rashid MbBchBoA 《Journal of forensic sciences》2023,68(6):2103-2115
The onus of proof in criminal cases is beyond any reasonable doubt, and the issue on the lack of complete internal validation data can be manipulated when it comes to justifying the validity and reliability of the X-chromosomal short tandem repeats analysis for court representation. Therefore, this research evaluated the efficiency of the optimized 60% reduced volumes for polymerase chain reaction (PCR) amplification using the Qiagen Investigator® Argus X-12 QS Kit, as well as the capillary electrophoresis (CE) sample preparation for blood samples on Flinder's Technology Associates (FTA) cards. Good-quality DNA profile (3000–12,000 RFU) from the purified blood sample on FTA card (1.2 mm) were obtained using the optimized PCR (10.0 μL of PCR reaction volume and 21 cycles) and CE (9.0 μL Hi-Di™ Formamide and 0.3 μL DNA Size Standard 550 [BTO] and 27 s injection time) conditions. The analytical and stochastic thresholds were 100 and 200 RFU, respectively. Hence, the internal validation data supported the use of the optimized 60% reduced PCR amplification reaction volume of the Qiagen Investigator® Argus X-12 QS Kit as well as the CE sample preparation for producing reliable DNA profiles that comply with the quality assurance standards for forensic DNA testing laboratories, while optimizing the analytical cost. 相似文献
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Commentary on: Intarapanich NP,McCobb EC,Reisman RW,Rozanski EA,Intarapanich PP. Characterization and comparison of injuries caused by accidental and non‐accidental blunt force trauma in dogs and cats. J Forensic Sci 2016 Jul;61(4):993–9. 下载免费PDF全文
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Evaluation of Skin Surface as an Alternative Source of Reference DNA Samples: A Pilot Study 下载免费PDF全文
Mohammed H. Albujja M.Sc. Abdul Aziz Bin Dukhyil Ph.D. Abdul Rauf Chaudhary M.Phil. Ahmed Ch. Kassab Ph.D. Ahmed M. Refaat Ph.D. Saranya Ramesh Babu M.Sc. Mohammad K. Okla Ph.D. Sachil Kumar Ph.D. 《Journal of forensic sciences》2018,63(1):227-233
An acceptable area for collecting DNA reference sample is a part of the forensic DNA analysis development. The aim of this study was to evaluate skin surface cells (SSC) as an alternate source of reference DNA sample. From each volunteer (n = 10), six samples from skin surface areas (forearm and fingertips) and two traditional samples (blood and buccal cells) were collected. Genomic DNA was extracted and quantified then genotyped using standard techniques. The highest DNA concentration of SSC samples was collected using the tape/forearm method of collection (2.1 ng/μL). Cotton swabs moistened with ethanol yielded higher quantities of DNA than swabs moistened with salicylic acid, and it gave the highest percentage of full STR profiles (97%). This study supports the use of SSC as a noninvasive sampling technique and as a extremely useful source of DNA reference samples among certain cultures where the use of buccal swabs can be considered socially unacceptable. 相似文献
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The aim of this paper is to examine whether changes in nominal oil prices (Brent and West Texas Intermediate (WTI)) affect the stock market returns in the context of an emerging market framework. The Autoregressive Distributed Lag bounds testing approach of cointegration is used to test for the long run relation between the two variables, where the daily stock market index return is calculated using the first difference in the natural logarithms of stock market index. Further, we test for the stability of the cointegration relationship by examining the sensitivity analysis where diagnostic tests for serial correlation (namely the Breusch–Godfrey serial correlations LM test) and cumulative sum of recursive residuals (CUSUM) are employed. Using daily data from January 3, 2000 to December 9, 2015, the findings suggest that there is long run integration between oil prices and stock returns series in which the daily oil price shocks have a negative impact on stock returns. The highly significant error correction coefficient indicates high rate of convergence to equilibrium. In addition, the Toda and Yamamoto (J Econom 66(2):225–250, 1995) Granger non‐causality test indicates significant bidirectional causality between stock market returns and Brent nominal oil price, meanwhile there is unidirectional causality running from WTI oil price to stock market returns. These findings are, up to some extent, meaningful for investors, portfolio managers and policy makers. 相似文献
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Karen Dawisha Joseph Frankel Alan M James Ashok Kapur Peter Calvert Mohammad A Qadeer 《Third world quarterly》2013,34(3):137-179
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