Development and validation of the AmpFℓSTR® Identifiler® Direct PCR Amplification Kit: a multiplex assay for the direct amplification of single-source samples

Abstract: The AmpF?STR^® Identifiler^® Direct PCR Amplification Kit is a new short tandem repeat multiplex assay optimized to allow the direct amplification of single‐source blood and buccal samples on FTA^® card without the need for sample purification and quantification. This multiplex assay has been validated according to the FBI/National Standards and SWGDAM guidelines. Validation results revealed that slight variations in primer concentration, master mix component concentration, and thermal cycling parameters did not affect the performance of the chemistry. The assay’s sensitivity was demonstrated by amplifying known amounts of white blood cells spotted onto FTA^® cards, and the assay’s specificity was verified by establishing minimal cross‐reactivity with nonhuman DNA. No effect on the age of the sample stored on the FTA^® substrate was observed and full concordance was established in the population study. These findings of the validation study support the use of the Identifiler^® Direct Kit for forensic standards and database samples genotyping.     

Personality Profiles of Special Force Police Officers

The present study was aimed at investigating whether different personality profiles could be found among Italian police Special Force (SF) officers. Our hypothesis was that different personality profiles would be found, and that they would differ with respect to psychological and organizational functioning. This conjecture was tested on anonymously collected Big Five scores of all members of a 'Reparto Mobile' unit (n?=?289) by means of a Two-Step cluster analysis. Two distinct personality profiles were found: two-thirds of participants showed a personality profile that was quite similar to the general population, with the exception of a higher self-reported emotional stability and self-deceptive enhancement, while the remaining third showed higher levels of any personality domain than both the other cluster and the general population. These officers also endorsed lower scores in measures of depression, anxiety, professional exhaustion, loss of empathy, and higher scores in a measure of organizational resilience, suggesting that they may represent the most suitable personnel for the delicate tasks their unit usually has to accomplish. In addition, self-deceptive enhancement emerged as a likely personality trait of police SF officers.     

The age of reason: An examination of psychosocial factors involved in delinquent behaviour

Purpose. Delinquent behaviour among children and adolescents is escalating at a considerable rate. This has led to calls to lower the Age of Criminal Responsibility (ACR); however there is limited research on which to base such a decision. The present study addressed this omission by (1) assessing whether or not children can accurately distinguish right from wrong in relation to ‘real‐life’ transgressions and (2) investigating psychosocial factors that may constrain children from acting in accordance with their knowledge of what is right and wrong. Methods. A total of 452 students were recruited from five schools in Sydney, Australia. Forty percent of participants were younger children recruited from Year 5 classes in primary school (mean age of 10.49), and 60% were older children from Year 8 classes in high school (mean age of 14.29). All students completed a questionnaire measuring their understanding of right and wrong, their level of moral disengagement and delinquent behaviour, and their perceived self‐efficacy relating to academic achievement, empathy, and resistance to peer pressure. Results. The majority of children in both age groups demonstrated knowing the difference between right and wrong in relation to ‘real‐life’ transgressions. Further analyses using structural equation modelling (analysis of moment structures, AMOS) revealed that children who engaged in delinquent behaviour were unable to exercise this knowledge appropriately to regulate their behaviour. They were less able to resist peer pressure for transgressive conduct, had low levels of empathic and academic self‐efficacies, and disengaged from moral standards. Conclusions. Implications for policy change and future research directions are proposed.     

Politics and the Public in Rawls' Political Liberalism

This paper is a critical discussion of a number of related themes in John Rawls' Political Liberalism. First, it considers whether Rawls' recent statement of his position proceeds from an adequate methodology for political theory. In particular, it questions whether Rawls has succeeded in accommodating both universalist, analytic and particularist, interpretive aspects of the political theoretical enterprise. Second, it engages in critical analysis of the conceptions of the political and the public which lie at the core of Rawls' theory. In this part of the paper, an important though not exclusive focus will be certain questions raised by Susan Moller Okin and other feminist critics of Rawls about the internal consistency of his conception of justice. It is argued that Political Liberalism neither addresses these questions explicitly nor, contrary to Okin's view, provides implicit conceptual tools which could allow a sympathetic interpreter of Rawls to do so. The direction of the argument will suggest certain preconditions for the development of a more substantively and methodologically adequate approach to political theory.     

Book reviews

The Partition of the Steppe. The Struggle of the Russians, Manchus, and the Zunghar Mongols for Empire in Central Asia, 1619–1758: A Study in Power Politics Fred W. Bergholz New York: Peter Lang, 1993, vi + 522 pp, hardcover

On Secret Service East of Constantinople: the plot to bring down the British Empire Peter Hopkirk London: John Murray, 1994. xvi, 431 pp. illus., £19.99

The Soviet Union and its Southern Neighbours: Iran and Afghanistan Mikhail Volodarsky London: Frank Cass Publishers, 1994, 196 pp, $37.50

Moscow's Lost Empire Michael Rywkin Armonk, New York: M.E. Sharpe, 1994, 214 pp, biblio, index

Russia and the Third World in the Post‐Soviet Era Mohiaddin Mesbahi (editor) Gainesville, USA: University Press of Florida, 1994, 414 pp, $49.95 cloth, $19.95/£18 paper

Central Asia and the World: Kazakhstan, Uzbekistan, Tajikistan, Kyrgyzstan, Turkmenistan Michael Mandelbaum (editor) New York: Council on Foreign Relations Press, 1994, viii, 251 pp, $16.95 paperback     

Growing up in the culture of slenderness: Girls'' experiences of body dissatisfaction

This paper reports two group-interviews of young women aged 8 and 13 years. These young women were dissatisfied with their bodies and described ideal figures that matched the slim adult ideal. The younger girls were not dieting but were involved in helping their parents to diet. Adolescents had avoided “fattening” foods and would be concerned if they put on half a stone in weight. Eating (particularly sweet food) was used as a means of comfort and to relieve boredom by young women in both age groups. Results are similar to those of Nickie Charles and Marion Kerr (1986) who interviewed adult women. It is concluded that children as young as 8 years give accounts of body dissatisfaction that are similar to those of adult women, and that suggest acceptance of the slim adult ideal. Implications are discussed in relation to cultural influences on young women that encourage objectification and criticism of the body.     

Shadow Writing and Participant Observation: A Study of Criminal Justice Social Work Around Sentencing

The study of decision‐making by public officials in administrative settings has been a mainstay of law and society scholarship for decades. The methodological challenges posed by this research agenda are well understood: how can socio‐legal researchers get inside the heads of legal decision‐makers in order to understand the uses of official discretion? This article describes an ethnographic technique the authors developed to help them penetrate the decision‐making practices of criminal justice social workers in writing pre‐sentence reports for the courts. This technique, called ‘shadow writing’, involved a particular form of participant observation whereby the researcher mimicked the process of report writing in parallel with the social workers. By comparing these ‘shadow reports’ with the real reports in a training‐like setting, the social workers revealed in detail the subtleties of their communicative strategies embedded in particular reports and their sensibilities about report writing more generally.