Validation of the MiniFiler™ Kit in archaeological samples

We evaluate the usefulness of MiniFiler™ Kit in the field of ancient DNA. A set of samples belonging to different locations from Iberian Peninsula, with ages ranging from Neolithic to XVII century, was tested. Results could be replicated in only one burial site, probably due to the taphonomic conditions. Other cases could only produce partial or none genetic profiles.     

Selective colorimetric determination of TNT partitioned between an alkaline solution and a strongly basic Dowex 1-X8 anion exchanger

The Meisenheimer anions formed from TNT in KOH solutions in alcohol or acetone were used in screening tests for TNT among possible nitro-explosives. The same reaction was used for the spectrophotometric assay of TNT in soil by CRREL (Cold Regions Research & Engineering Laboratory of the U.S. Army) method, also known as Jenkins' method, but the color stability was too dependent on the solution composition and the water tolerance was low, necessitating complete drying of soil samples (which may cause partial analyte decomposition) prior to analysis. This study reports the development of a colorimetric method based on the solid phase extraction (SPE) of the Meisenheimer anion formed from TNT and aqueous NaOH into a strongly basic anion exchange resin Dowex 1-X8 (OH(-) form). The orange-red color that developed both in the solid resin and solution phases was persistent for at least 1h. The resin was let to swell in alcohol, washed first with 1M aqueous NaOH, and then with H(2)O before use. To 5 mL of 4-400 ppm TNT solutions in 1:1 (v/v) acetone-water, 0.5 mL of 5% NaOH was added, diluted to 50 mL with 1:1 acetone-water, and the resulting solutions (containing the orange-red Meisenheimer anion of TNT) were agitated at room temperature with 0.9 g resin for < or =50 min. TNT exhibited a reasonably constant distribution coefficient between the resin and aqueous phases. The absorbance of the filtered solutions was measured against a reagent blank at 500nm. The TNT-loaded resins were regenerated with 1M HCl containing sodium sulfite. The calibration line of filtrate absorbance versus analytical concentration was linear over two orders of magnitude between 0.4 and 40 ppm TNT in final solution. Unlike Jenkins' method, the method was tolerant to 100-fold (by mass) of common soil anions like sulfate, nitrate, and chloride. The basic advantages of the developed colorimetric method over the similar CRREL/Jenkins' method may be summarized as color stability, water and common ion tolerance, lowering of the LOD, and widening of the linear range. As opposed to Jenkins' method, the charge-transfer bands characteristic to colorimetric detection were not obscured by the presence of water. The method is suitable for on-site applications, because the color developed in heterogenous solution was stabilized, and the method can be easily practiced with a portable colorimeter to large numbers of samples.     

Molecular genetics of sudden cardiac death

Sudden cardiac death (SCD) is one of the most common causes of death. An important number of sudden deaths, especially in the young, are due to genetic heart disorders, both with structural and arrhythmogenic abnormalities. In recent years, significant advances have been made in understanding the genetic basis of SCD. Identification of the genetic causes of sudden death is important because close relatives are also at potential risk of having a fatal cardiac condition. A comprehensive post-mortem investigation is vital to determine the cause and manner of death and provides the opportunity to assess the potential risk to the family after appropriate genetic counselling. In this paper, we present an update of the different genetic causes of sudden death, emphasizing their importance for the forensic pathologist due to his relevant role in the diagnosis and prevention of SCD.     

Migration and Healthcare Reforms in Spain: Symbolic Politics,Converging Outputs,Oppositions from the Field

ABSTRACT

Migrants’ healthcare entitlement represents a conflictive issue in the political battlefield, with research pointing towards the determinant role of party politics in determining policy outputs. Addressing the 2012 healthcare reform and 2018 counter-reform adopted in Spain by a right-wing and left-wing government respectively and drawing on qualitative analysis of parties’ discourses and policy measures, we argue that ideological differences along the healthcare-migration nexus were overemphasised to play symbolic politics. Partisan competition had less impact on actual outputs, while clashes between the central and regional governments, path-dependent practices and opposition from multiple venues played a central role in the policymaking process.     

Baring it all for Activism: The 1985 Naked Protest in Mexico

This article analyses the 1985 naked protest carried out by silver miners of Pachuca, Mexico. This singular form of resistance, the first in Mexican labour history and organised by a dissident group within the miners' union called Liberación Minera (Miners Liberation), forced management to recognise and temporarily solve some of the miners' grievances. The naked protest unveiled the shady practices of the miners' employer, the state-owned Compañía Real del Monte y Pachuca, which refused to provide work clothes and safety equipment to miners. It also pointed to the miners' union leadership's complicity in the deterioration of labour conditions. Part of the miners' naked protest success had to do with the support that they gained from members of the left-wing press who used the protest to offer an early critique of Mexico's neoliberal policies. The 1985 naked protest occurred during one of Mexico's most severe economic crises and only four years before the company became privatised. This protest is one of the last examples of organised labour resistance before industries closed down and fired thousands of workers.     

Domestic Contestation and Presidential Prerogative in Colombian Foreign Policy

The study of Colombian foreign policy emphasises external constraints and presidential prerogative in foreign policymaking. Drawing on insights from recent foreign policy analysis literature and evidence from several cases (Plan Colombia, US military bases, free trade talks with China, and ICJ arbitration of a maritime border with Nicaragua), this article challenges commonplace presidentialist assumptions. A novel model of ‘contested presidentialism’ better captures how Colombian domestic actors mobilise to raise political costs to block or modify presidential preferences. When the opposition fails to raise costs, presidentialist assumptions apply. Otherwise, presidents respond strategically by abandoning policies or substituting second-best alternatives.     

Media Discourse and the Quality of Democracy in Serbia after Milošević

Abstract

The article examines the quality of democracy in Serbia via the quantitative analysis of media discourse. It reveals robust competition, participation and accountability in the first decade after regime change, followed by major recent decline, thus showing that expert assessments from influential indices of democracy underrated democratic quality in the former period and overrated it later. Also revealed are the advantages of complementing expert assessments with those based on media discourse. The content analysis examined 1,921 coded items from print and electronic media coverage of major political conflicts at strategic points in the country’s democratisation.