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171.
172.
Effectiveness of Coupled Application of AmpFℓSTR Yfiler Kit and Reduced Size Y‐chromosomal Short Tandem Repeat Analysis for Archeological Human Bones
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Chang Seok Oh M.Sc. Soong Deok Lee M.D. Ph.D. Kyoung‐Jin Shin D.D.S. Ph.D. Dong Hoon Shin M.D. Ph.D. 《Journal of forensic sciences》2016,61(2):430-438
The AmpF?STR Yfiler PCR Amplification (Yfiler) kit continues to be improved for a better analytical efficiency in cases of highly degraded DNA. The authors endeavored to determine whether coupling of the Yfiler kit with supplemental multiplex amplification of some Y‐STR loci is a more efficient analytical mode for poorly preserved human femurs (n = 15) discovered at Korean archeological sites. To reveal locus profiles not easily obtained by Yfiler analysis, custom‐designed primers were adopted for the DYS390, DYS391, DYS392, DYS438, DYS439, and DYS635 loci. The success rate for 16 Y‐STR locus profiles obtained from the 15 femurs was improved from 18.33% (in the use of Yfiler kit only) to 49.17% (the coupled use of Yfiler and custom‐designed primers). In this study, the authors established that the custom‐designed primers offer a markedly improved success rate for obtainment of Y‐STR profiles from degraded aDNA not easily identified by sole use of the Yfiler assay. 相似文献
173.
Application of mRNA Expression Analysis to Human Blood Identification in Degenerated Samples that were False‐negative by Immunochromatography,,
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Shusaku Matsumura M.S. Aya Matsusue Ph.D. Brian Waters M.S. Masayuki Kashiwagi M.D. Ph.D. Kenji Hara Ph.D. Shin‐ichi Kubo M.D. Ph.D. 《Journal of forensic sciences》2016,61(4):903-912
Forensic laboratories are often faced with cases in which methamphetamine hydrochloride‐mixed blood is unable to be identified as human blood by immunochromatography against human hemoglobin A0. The application of mRNA expression analysis to samples that showed a false‐negative with immunochromatography was investigated as an alternative approach that did not depend on the antigen–antibody reaction. Real‐time PCR was used to examine the expression levels of blood markers such as glycophorin A, spectrin beta, and hemoglobin beta. Hemoglobin beta was the only marker that was specifically detected in blood, while glycophorin A was useful for determining human specificity. Hemoglobin beta showed good detection sensitivity and was detectable in 37‐year‐old blood stains. Hemoglobin beta was exclusively detectable in methamphetamine hydrochloride‐mixed blood stains. Detergents and disinfectants did not significantly influence mRNA markers. The proposed mRNA expression analysis was suitable for human blood identification as an alternative method to immunochromatography. 相似文献
174.
Roy Liff 《国际公共行政管理杂志》2013,36(8):474-483
This article examines the unintended consequences following implementation of a new public management (NPM) reform—a performance-based salary system—in two Swedish public schools. Headmasters and central office personnel were interviewed. The expected reform results at last appeared a decade after implementation when salary-setting procedures adopted the bureaucratic framework. Despite the common view that NPM reforms, owing to unintended consequences, fail because they adapt poorly to the Weberian control regime at public organizations, this article argues that the unintended consequences of an NPM reform can drive the “bureaucratic” organization even when there is no evidence of debureaucratization. 相似文献
175.
Dong‐Ik Shin 《The Pacific Review》2013,26(4):504-522
Abstract As its economy has become near to collapse, North Korea has tried to avoid direct contacts with South Korea because of the ‘absorption phobia’. Instead, the North has made continuous efforts to improve its relations only with the United States, seeking a guarantee for its survival. Given this circumstance, this paper argues that useful multilateral approaches such as KEDO and Four‐Party Talks will contribute to improving inter‐Korean relations. Thus, it would be sensible to explore every possible way (even through multilateral mechanisms) until both Koreas make a breakthrough for the deadlocked inter‐Korean CBMs. But the multilateral CBMs constitute a transitional and complementary role as South and North Korea should be primarily responsible for addressing major problems such as reunification. Among the multilateral approaches, the Four‐Party Talks will be a most useful mechanism which will enable the two Koreas to resume dialogue for the peace and reunification on the Korean Peninsula. In this peace process, more positive roles of major powers are also requested. 相似文献
176.
Eui Hang Shin 《发展研究杂志》2013,49(4):398-412
Data from 63 selected countries of the world at two different points in time, 1958 and 1968, are used to test a path model in which infant mortality acts as an intermediate variable between two composite indicators of economic and social development and the fertility rate. The model is tested with both cross‐sectional and longitudinal data. In order to ascertain the appropriate time dimension in which the independent variables influence the trends of fertility, the results from an instantaneous model are compared with those from a lagged effect model. 相似文献
177.
Around 2007 Turkey became a regional power in the Middle East, a status it has maintained at least until the outset of the Arab Revolt in 2011. To understand why Turkey only became a regional power under the Muslim akp government and why this happened at the specific point in time that it did, this article highlights the self-reinforcing dynamics between Turkey’s domestic political-economic transformation in the first decade of this century and the advantageous regional developments in the Middle East at the same time. It holds that this specific linkage—the ‘Ankara Moment’—and its regional resonance in the neighbouring Middle East carries more transformative potential than the ‘Washington Consensus’ or the ‘Beijing Consensus’ so prominently discussed in current global South politics. 相似文献
178.
Chung U Shin KJ Park MJ Kim NY Yang WI Cho SH Lee HY 《Forensic science international》2007,168(2-3):e51-e53
For highly degraded DNA samples of forensic casework, new miniSTR systems have been developed to supplement the current STR systems. In the present study, nine miniSTR loci were analyzed in 300 unrelated Koreans using three multiplex PCR systems (multiplex I: D10S1248, D14S1434 and D22S1045; multiplex II: D1S1677, D2S441 and D4S2364; and multiplex III: D3S3053, D6S474 and D20S482), and allele frequencies and forensic parameters were calculated. These data demonstrated that D10S1248, D2S441, D22S1045, D14S1434, and D6S474 are as highly informative as the CODIS STRs suggesting that the miniSTRs could be useful for forensic analysis of degraded DNA. 相似文献
179.
Analyses were conducted to examine the extent of overlap and predictive strength of multiple forms of child maltreatment and
stressors internal and external to the family. Findings suggest a strong association between child maltreatment (physical
and sexual abuse, neglect, and exposure to domestic violence) and stressors (family conflict, personal problems of parents,
and external constraints on the family). After controlling for stressors, family socioeconomic status, and child gender, a
general construct of child maltreatment was predictive of internalizing and externalizing behaviors in adolescents. Specific
effects of child physical and sexual abuse were revealed in these longitudinal structural equation model tests.
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Todd I. HerrenkohlEmail: |
180.