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A 40-year-old pedestrian was involved in a road traffic accident. He lost consciousness immediately and remained comatose for 6 months until he died of a bronchopneumonia. Examination of the fixed brain revealed the late results of diffuse axonal injury. Not all the features were readily visible macroscopically, but required a limited number of histological sections from readily predictable sites for demonstration. Special stains played only a confirmatory role. This case illustrates some of the problems in diagnosing one of the commoner patterns of brain damage in head injury, and shows how the problem may best be approached in practice. 相似文献
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Véronique Bourdon Ph.D. Carolyn Ng B.S. Jessica Harris M.S. Mechthild Prinz Ph.D. Eli Shapiro Ph.D. 《Journal of forensic sciences》2014,59(4):1057-1063
Sequencing mitochondrial DNA hypervariable regions I and II (HVI and HVII) is useful in forensic missing person and unidentified remains cases. Improvements in ease and sensitivity of testing will yield results from more samples in a timely fashion. Routinely, amplification of HVI and HVII is followed by Sanger sequencing using the BigDye® Terminator v3.1 Cycle Sequencing kit (Applied Biosystems) using 4 μL of ready reaction mix (RRM). Each sequencing reaction is then purified through column filtration before capillary electrophoresis. Using lower amounts of RRM (2 μL or 1 μL) and purification using BigDye® XTerminator? (Applied Biosystems) instead of columns showed no loss of sequence length and increased the quality and the sensitivity of testing, allowing HVI and HVII typing from mitochondrial genome equivalent to 125 fg of nuclear DNA, or 100 pg of HVI/HVII amplicons. Using this methodology, testing can be completed in 1 day, and the cost of testing is reduced. 相似文献
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Bryn Rosenfeld Kosuke Imai Jacob N. Shapiro 《American journal of political science》2016,60(3):783-802
When studying sensitive issues, including corruption, prejudice, and sexual behavior, researchers have increasingly relied upon indirect questioning techniques to mitigate such known problems of direct survey questions as underreporting and nonresponse. However, there have been surprisingly few empirical validation studies of these indirect techniques because the information required to verify the resulting estimates is often difficult to access. This article reports findings from the first comprehensive validation study of indirect methods. We estimate whether people voted for an anti‐abortion referendum held during the 2011 Mississippi General Election using direct questioning and three popular indirect methods: list experiment, endorsement experiment, and randomized response. We then validate these estimates against the official election outcome. While direct questioning leads to significant underestimation of sensitive votes against the referendum, indirect survey techniques yield estimates much closer to the actual vote count, with endorsement experiment and randomized response yielding the least bias. 相似文献
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Using integrated administrative data from criminal justice and social service systems can harness information in meaningful ways that transcend traditional “silos” and allow communities to focus collective attention on important social issues that cross systemic boundaries. Despite recent advances in use of integrated administrative data, practical information to promote adoption by new users is lacking. Here we provide an introduction to potential uses of integrated administrative data for criminal justice researchers, including general benefits of using integrated data as well as implications for innovative research design. We describe a case example of data integration through a state data warehouse for a federally funded project on impact of incarceration on families. The project utilizes data from eight agencies (corrections, juvenile justice, mental health, substance use, social services, health, education, and environmental control) and includes development of an Online Analytical Processing cube. We draw from lessons learned to provide specific recommendations for developing researcher-practitioner partnerships that use integrated administrative data to improve translational criminal justice research and evidence-based practice and policy. 相似文献
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Leff SS Thomas DE Shapiro ES Paskewich B Wilson K Necowitz-Hoffman B Jawad AF 《Journal of school violence》2011,10(2):165-184
The climate of school classrooms, shaped by a combination of teacher practices and peer processes, is an important determinant for children's psychosocial functioning and is a primary factor affecting bullying and victimization. Given that there are relatively few theoretically-grounded and validated assessment tools designed to measure the social climate of classrooms, our research team developed an observation tool through participatory action research (PAR). This article details how the assessment tool was designed and preliminarily validated in 18 third-, fourth-, and fifth-grade classrooms in a large urban public school district. The goals of this study are to illustrate the feasibility of a PAR paradigm in measurement development, ascertain the psychometric properties of the assessment tool, and determine associations with different indices of classroom levels of relational and physical aggression. 相似文献
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Joe Shapiro 《Socialism and Democracy》2013,27(2):131-135
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