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Katie M. Horsman-Hall Yvette Orihuela Stephanie L. Karczynski Ann L. Davis Jeffrey D. Ban Susan A. Greenspoon 《Forensic Science International: Genetics Supplement Series》2009,3(4):242-250
Fired cartridge cases are a common type of evidence found at crime scenes. However, due to the high chamber temperatures and touch nature of this evidence, DNA testing is not commonly sought because it is believed DNA is only present in low levels, whether it is due to initial low levels of DNA and/or DNA degradation from the heat or inhibition of the PCR reaction. Moreover, very few laboratories report STR typing success with fired cases. This study focused on obtaining STR profiles from fired cartridge cases using the AmpFℓSTR® MiniFiler™ kit, which is designed to amplify DNA from low level, inhibited, and degraded samples. Comparisons to other STR amplification kits were also conducted. In attempt to simulate casework, random individuals loaded cartridges into a firearm. DNA was recovered from the fired cartridge cases using the double swab technique and extracted using an automated large volume DNA IQ™ method. Initially, testing focused on known shedders handling cartridges for 30 s prior to firing. A significantly greater number of alleles was obtained following amplification with the MiniFiler™ kit versus the PowerPlex® 16 BIO kit. No alleles were observed using the Identifiler® kit. In an attempt to better simulate casework, a random selection of laboratory personnel handled shotshells for as long as needed to load and fire the weapon. In this mock sample study, the MiniFiler™ kit successfully amplified an average of 22% of expected alleles from DNA recovered from shotshell cases versus the PowerPlex® 16 BIO kit where an average of 7% of alleles were observed. However, the total number of alleles obtained from the two kits was not significantly different. The quality of the DNA obtained from fired cases was studied with evidence of inhibition in at least 11% of shotshell case samples. After swabbing the head and the hull of three shotshell cases separately, a significantly greater number of alleles was obtained from the hull as opposed to the head of the fired shotshell case. In addition, after firing, various internal firearm surfaces were swabbed, including the chamber of barrel, ejection port, and breechface, in an attempt to obtain amplifiable DNA. DNA was obtained from the chamber of the barrel and was amplifiable using the MiniFiler™ kit, although mixtures were obtained with extensive drop-in and drop-out making this analysis unlikely to aid an investigation. 相似文献
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Susan A. Munkres 《Law & social inquiry》2008,33(2):447-472
Sociologists of law have long been concerned with the effectiveness of rights; the emergence of diversity training in the 1990s spurred renewed attention to questions of how laws are enacted in daily life. Much scholarship has constructed the managerialization of civil rights law and popularization of diversity concepts as diluting efforts to redress structural discrimination. In studying diversity and antiharassment trainings in practice, I argue that these are sites where civil rights find expression of their obligations, and I find that much of the "dilution" of content stems from diversity trainers' efforts to negotiate with the resistance of trainees to their new obligations under civil rights law. The trainees evince a variable legal consciousness in relationship to this legality of rights-promotion, to which they are being exposed in these trainings; the findings suggest further research is needed into the legal consciousness of the privileged. 相似文献
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When you have a rational discussion of what to do with the Israeli settlements, how do you factor in the irrational, the deeply held beliefs of people with varying views? As we witnessed at the conference, when people speak about the Israeli settlements, they do so with emotion, using such phrases as “Messianic spirit,”“longing for homeland,”“compassionate revenge,” and “the destruction of dreams.” The land in question is precious to two different groups, who each imbue the land with their own narrative and fervor. In fact, at the very heart of what makes the settlements issue so complex and seemingly intractable is its psychological dimensions — the mental and emotional factors that permeate the discussion at every level. 相似文献
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Chun Bun Lam Anna R. Solmeyer Susan M. McHale 《Journal of youth and adolescence》2012,41(12):1657-1670
Although socioemotional competencies have been identified as key components of youths’ positive development, most studies on empathy are cross-sectional, and research on the role of the family has focused almost exclusively on parental socialization. This study examined the developmental course of empathy from age 7 to 14 and the within-person associations between sibling warmth and conflict and youths’ empathy. On three occasions across 2?years, mothers, fathers, and the two eldest siblings from 201 White, working- and middle-class families provided questionnaire data. Multilevel models revealed that, controlling for youths’ pubertal status and parental education, girls’ empathy increased during the transition to adolescence and then leveled off, but boys’ lower levels of empathy remained relatively unchanged. Moreover, controlling for parental responsiveness and marital love, at times when firstborns and second-borns reported more sibling warmth and less sibling conflict than usual, they also reported more empathy than usual. The within-person association between sibling warmth and empathy also became stronger over time. Findings highlight gender differences in empathy development and the unique role of siblings in shaping each other’s socioemotional characteristics during adolescence. 相似文献
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Susan B. Boyd 《Feminist Legal Studies》2010,18(2):137-158
This article explores the tensions between autonomy and expectations of mother-caregivers, in the context of normative trends
in post-separation parenting law. Going back to first principles of feminism, the article asks what scope for autonomy there
is for modern mothers in the face of socio-legal norms that prioritise shared parenting. The very relationship between mother-caregivers
and children illustrates the important connection between relationships and autonomy: the caregiving that mothers provide
enables children to become autonomous persons yet, at the same time, this caregiving relationship constrains maternal autonomy.
In the current context that encourages shared parenting, the potential for maternal autonomy may be even more compromised—a
deep irony in a supposedly post-feminist era. A responsible mother is now expected to nurture a child’s relationship with
the father, unless he is proven to be harmful. The ability of women to be at all autonomous from the fathers of their children
in the face of this normative expectation is dubious, even when the adults live separately. Moreover, the dominance of the
heterosexual and patriarchal family—always a challenge for women’s autonomy—is reproduced in this imposition of equal parenting
in the name of children’s rights. This article uses a contextual approach to relational autonomy to point to an approach that
might challenge the normative climate of shared parenting. 相似文献