Identification of a skeleton using DNA from teeth and a PAP smear.

Identification of unknown living or deceased persons using dental treatment records is an established forensic technique. However, some cases remain unidentified, especially when antemortem dental records are not available for comparison to postmortem dental records. Cytological smears have been previously reported to be potential sources of DNA reference samples which can be compared to DNA recovered from found human remains. The case described here involves an adult skeleton which exhibited extensive, complex dental restorative treatment. A putative identification of the found skeleton as a missing woman was established using circumstantial evidence found at the scene. However, it became important to establish a positive identification using reliable scientific methods. When it was discovered that antemortem dental records were not available because the treatment was completed in another country and the treating dentist could not be found, cytological smears stained with Papanicolaou (PAP) stain obtained from the putative decedent's medical records were used as a reference DNA sample. DNA was recovered from the teeth of the skeleton using cryogenic grinding. Comparison of the genotypes resulted in the conclusion that the DNA originated from the same source. The use of PAP smears in this way is seen as a valuable resource in cases where positive identification using traditional dental and medical records is not possible.     

Inferring ancestral origin using a single multiplex assay of ancestry-informative marker SNPs

Tests that infer the ancestral origin of a DNA sample have considerable potential in the development of forensic tools that can help to guide crime investigation. We have developed a single-tube 34-plex SNP assay for the assignment of ancestral origin by choosing ancestry-informative markers (AIMs) exhibiting highly contrasting allele frequency distributions between the three major population-groups. To predict ancestral origin from the profiles obtained, a classification algorithm was developed based on maximum likelihood. Sampling of two populations each from African, European and East Asian groups provided training sets for the algorithm and this was tested using the CEPH Human Genome Diversity Panel. We detected negligible theoretical and practical error for assignments to one of the three groups analyzed with consistently high classification probabilities, even when using reduced subsets of SNPs. This study shows that by choosing SNPs exhibiting marked allele frequency differences between population-groups a practical forensic test for assigning the most likely ancestry can be achieved from a single multiplexed assay.     

No room for Asia-Pacifists?

When this Journal decided to run a special issue on IP in theAsia-Pacific region, it was following the conventional wisdomof the profession and the marketplace. For many years, the conceptof the Asia-Pacific sold conferences and printed publications.And it was on this assumption that JIPLP based its decisionto give over its March issue for coverage of that zone. However,there comes a point in the life of     

Making the innocent pay

Even those of us who enter the legal profession for reasonsof personal advancement or greed can spot an injustice whenwe see it. As students and raw recruits to the profession, weshare with our clients the notion that, in any cause, one sideis ‘right’ and the other ‘wrong’. Toallow the party in the wrong to vanquish the party in the rightwould be unjust, notwithstanding the fact that the law permitsit. In time our perspective mellows: we learn to appreciatethat     

IP litigation, the new money-spinner

It is a truism that intellectual property litigation costs alot of money. It can be ruinously expensive even for the victor,never mind the beaten, bloodied loser. Much effort has beendirected at reducing the cost of combat in court: streamliningthe trial process, rocket dockets, better case management, toname a few, have produced some relief for costly litigationbut no cure. However, since the reward for the winner is sooften the opportunity of either enforcing a