Rapid isolation of some antiepileptic hydantoins and their analogues with Sep-Pak C18 cartridges and capillary gas chromatography with splitless injection

A simple and rapid method for isolation of seven antiepileptics (2 hydantoin, 2 oxazolidin, and 3 suximide derivatives) from urine and plasma is presented. Urine and plasma (1 ml) samples containing seven antiepileptics were mixed with distilled water (4 ml), and the sample solution was poured into a pretreated Sep-Pak C18 cartridge; this was washed with water and chloroform/methanol was passed through it to elute the antiepileptics. The eluate was mixed with isoamyl acetate and evaporated under a stream of N2. The drugs were detected by gas chromatography with fused silica capillary columns, splitless injection and flame ionization detection. Separation of the seven antiepileptics from each other and from impurities was satisfactory with the use of an SPB-1 capillary column. The detection limit for the seven antiepileptics with the present method was 0.1-1.0 microgram/ml urine or plasma. The recovery of the drugs from urine and plasma was more than 70% and 50%, respectively.     

Positive and negative ion mass spectrometry of benzophenones, the acid-hydrolysis products of benzodiazepines

Positive electron impact (EI), positive chemical ionization (CI), and negative CI mass spectra of 14 benzophenones are presented. In the positive EI mode, intense molecular peaks appeared for most compounds; some other peaks due to splitting at both sides of the carbonyl group also appeared. In the positive CI mode, [M + 1]+ quasi-molecular ions together with [M + C2H5]+ peaks were observed for all compounds; some fragment peaks were common to those in the positive EI mode. In the negative CI mode, the spectra were much simpler than those in the positive EI or CI mode. In the 1 Torr negative CI mode, some spectra showed only single molecular anions; in the 0.01 Torr negative CI mode, halogen or nitro peaks appeared in addition to the molecular anions. An extraction procedure for benzophenones from human urine and plasma after heating in strong acid, and their separation by gas chromatography (GC) are also presented to serve for their actual identification by GC/mass spectrometry.     

Positive and negative ion mass spectrometry of antiepileptic hydantoins and their analogs

Positive ion electron impact (PIEI), positive ion chemical ionization (PICI), and negative ion chemical ionization (NICI) mass spectra of seven compounds of hydantoins and their analogs are presented; their probable fragmentation modes are also presented. In the PIEI mode, intensities of molecular ions differed according to different compounds. Cleavage at outsides of both carbonyl groups was commonly observed for all compounds. In the PICI mode, all compounds showed [M + 1]+ quasi-molecular ions constituting the base peaks. In the NICI mode, [M - 1]- quasi-molecular anions were the base peaks except for trimethadione and paramethadione. All negative spectra showed anions at m/z 42 due to [NCO]-; these peaks seem useful for screening of antiepileptics. An extraction procedure for the anti-epileptics from human urine or plasma, and their separation by gas chromatography (GC), are also presented to serve for their actual identification by GC/mass spectrometry.     

Detection of methamphetamine and amphetamine in a single human hair by gas chromatography/chemical ionization mass spectrometry

A detailed procedure of an extremely sensitive method for quantitation of methamphetamine and amphetamine in human hair by gas chromatography (GC)/chemical ionization (CI) mass spectrometry (MS) is presented. N-methylbenzylamine was used as an internal standard. The samples, after extraction with an organic solvent, were derivatized with trifluoroacetic anhydride before the GC/MS analysis. Quantitation was made with quasi-molecular ions of the derivatives by selected ion monitoring in the CI mode. The detection limit was about 10 pg in an injected volume. The high sensitivity enabled us to measure both stimulants in a single human hair in actual cases.     

An experimental study of the identification of the person of origin of a bloodstain by crossed immunoelectrophoresis

Crossed immunoelectrophoretic (CIEP) patterns were examined for the purpose of individualization of bloodstains. For comparison the areas of peaks were measured and ratios of the areas of corresponding peaks were calculated. To assess the dispersion of the ratios, the coefficient of variation (CV) was calculated as CV = 100 X standard deviation/mean of ratios. With 1-month-old bloodstains there were significant differences in the distributions of CV between the groups of the same and those of different origin. These results indicate that CIEP is useful as a means of identifying the origin of bloodstains.     

Liver damage in rats during acute carbon monoxide poisoning

Plasma leucine aminopeptidase (LAP) levels and respiration rates of isolated liver mitochondria were studied in carbon monoxide (CO)-poisoned rats sampled at respiratory arrest. An increase in LAP levels paralleled a decrease in the respiratory control ratio and the $\text{ADP}\text{O}$ ratio. The results suggest that the damage to mitochondria closely correlates with the liver damage in rats during acute CO poisoning.     

Identification of Arrhythmogenic Right Ventricular Cardiomyopathy‐Causing Gene Mutations in Young Sudden Unexpected Death Autopsy Cases

Arrhythmogenic right ventricular cardiomyopathy (ARVC) results in an increased risk of sudden death. We sought mutations of desmoglein‐2 (DSG2), desmoplakin (DSP), and plakophilin‐2 (PKP2) in 15 cases of sudden death whose causes of death could not be determined at autopsy. In three victims, mutations were identified in DSP. Two of these mutations were novel; one had previously been reported in a patient with ARVC that had been diagnosed clinically. Histological findings were not typical of ARVC; however, it was notable that these mutations were present in three of 15 cases, a relatively high proportion. The causal relationship between the mutations and ARVC is unclear, but the mutations might have been associated with faulty desmosomal proteins resulting in fatal arrhythmia. Combining information gathered by the traditional means of gross and histological examination with postmortem genetic analysis of young victims would assist in identifying their cause of death.     

Corruption,interest rates and business cycles: comparison of emerging economies

We examine the hypothesis that corruption in a country negatively influences the macroeconomy through an increase in the country-specific interest rate (interest rate shock). An empirical study estimated the contribution of the interest rate shocks to the variance in output growth at 5.1% in Mexico within the framework of stochastic growth models for small open economies. We replicate this study with the same dataset and investigate which parameters affect the contribution of the interest rate shocks to business cycles. Then, we estimate the same model for different emerging economies to investigate the relationship between the corruption level and macroeconomic contribution of the interest rate shocks. For this purpose, we use Transparency International’s Corruption Perceptions Index (CPI) to measure the corruption level. Finally, we investigate the correlation between the CPI and the estimated series of the interest rate shock. Our findings are as follows. First, the average size of the interest rate shocks is positively associated with the contribution of these shocks to the variability of output growth. Second, the average size of the interest rate shocks is also positively associated with the corruption level. Third, the estimated interest rate shock and the corruption level are positively correlated with each other. As we treat the corruption level as an exogenous variable in the model, these findings lead us to accept the hypothesis. The “Appendix” further clarifies a well-known hypothesis that the cycle is the trend in an emerging economy.     

Systematic Quantitative Literature Review on Criminological Theories in Asia

Empirical work on criminological theories in Asia has been increasing. However, few comprehensive and systematic reviews on the application of criminological theories in Asia have been conducted. Using a systematic quantitative literature review method on peer-reviewed English-language journal articles, we aim to provide an overview of the use of five major criminological theories in Asia: (1) strain; (2) social learning; (3) control; (4) routine activity; and (5) developmental and life-course. In particular, we address the following four questions: (1) how often are these theories tested in which region of Asia?; (2) what methodology is used to test these theories?; (3) to what extent are these theories supported in the Asian context?; and (4) what cultural uniqueness in the Asian context is taken into account in testing these theories, and what role and effect do they play in analysis and outcome? Findings indicate that the relationship between these theories and the Asian regions is skewed; many studies do not employ rigorous methodologies; these theories are either fully or partially supported in the Asian context; and only a few studies have analyzed the cultural uniqueness of the Asian context, and no effect of cultural uniqueness of the Asian context was found. Research implications for developing criminology in Asia are discussed.