Constructing STR Multiplexes for Individual Identification of Hungarian Red Deer

Red deer is the most valuable game of the fauna in Hungary, and there is a strong need for genetic identification of individuals. For this purpose, 10 tetranucleotide STR markers were developed and amplified in two 5‐plex systems. The study presented here includes the flanking region sequence analysis and the allele nomenclature of the 10 loci as well as the PCR optimization of the DeerPlex I and II. LD pairwise tests and cross‐species similarity analyses showed the 10 loci to be independently inherited. Considerable levels of genetic differences between two subpopulations were recorded, and F_ST was 0.034 using AMOVA. The average probability of identity (PI_ave) was at the value of 2.6736 × 10^?15. This low value for PI_ave nearly eliminates false identification. An illegal hunting case solved by DeerPlex is described herein. The calculated likelihood ratio (LR) illustrates the potential of the 10 red deer microsatellite markers for forensic investigations.     

Introduction to the Special Issue: New Forms of Intolerance in European Political Life

This introduction summarizes the contributions to the Special Issue that focus on the spread of intolerant and racist discourses in Denmark, Italy, Spain, Greece, and Hungary. Through a comparative approach, the issue argues that what has been decisive in this process is the role played by mainstream political parties that perceive intolerance against the “other” as the natural outcome of the failure of previous tolerant policies on immigrants and minorities. Even if brought forward with different argumentation in each case, intolerance is introduced in all five countries as a principled position under the pretext of protecting European citizens’ rights.