人体皮肤创伤组织细胞因子表达的时间相关性

目的探讨TGF-β1、b—FGF和VEGF在人体皮肤创伤组织中的表达变化及其与损伤时间的关系。方法收集15例机械性致伤的人体皮肤组织(受伤5—120min内死亡),常规制作组织切片后进行免疫组化染色,并统计分析TGF-β1、b—FGF和VEGF因子的表达情况。结果 TGF-β1在16—50min组表达升高,60～120min组呈阳性至强阳性表达;b—FGF在16—50min组表达升高明显,强阳性表达亦出现在60～120min组;VEGF仅在60—120min组出现阳性表达。统计学分析表明,TGF—β1和b—FGF的表达升高分别在受伤60~120min(P<0.01)和在16—50min(P<0.01)有显著性意义,VEGF表达升高只在60~120min(P<0.05)有显著性意义。结论 TGF-β1、b—FGF和VEGF的表达时间和表达强度与损伤时间有关;TGF-β1、b—FGF和VEGF的表达可为人体短存活期损伤时间的推断提供参考。     

大鼠急性心肌缺血zif/268蛋白表达及其意义

目的 探讨急性心肌缺血早期不同时间不同区域心肌细胞内原癌基因蛋白zif/268的表达变化,为心肌早期缺血死后诊断提供新指标。方法 建立心肌早期缺血模型,大鼠分为正常、缺血组。采用免疫组化SABC法研究心肌细胞核蛋白zif/268的累积情况。结果 缺血 60min后,缺血区大鼠心肌细胞有部分心肌细胞核呈弱阳性着色,以后随缺血时间延长核阳性增强。正常和缺血30min组及未缺血区心肌细胞核未见有阳性反应。HE染色无明显病理改变。结论 免疫组化染色法检测心肌细胞核zif/268的表达对急性心肌缺血的死后诊断是一种有价值有意义的手段。     

苯并二氮类药物及其主要代谢物的薄层色谱分析

目的建立饮料、血、尿中 11种常见苯并二氮类药物及尿中 7种主要代谢物的薄层色谱分析法(HPTLC)。方法分析物采用GDX10 1树脂进行固相萃取 ,乙醚作为洗脱溶剂。苯 :丙酮 (10∶6)等作为展开体系 ,改良碘化铋钾显色。结果所建方法绝对灵敏度 0 3～ 0 6μg,尿检出限 0 4～ 1 0 μg/ml、血检出限 0 6～ 1 0 μg/ml、饮料检出限 0 4～ 0 8μg/ml。结论HPTLC法简便、快速 ,适合作为常规毒物分析方法     

3个Y-STR的复合扩增及其单倍型

目的 建立复合扩增Y-STR基因座的体系,获得广东汉族人的单倍型频率。方法 复合扩增DYS439、DYS437和DYS434三个基因座,用聚丙烯酰胺凝胶电泳银染法进行基因分型,检测广东汉族327名无关男性个体的单倍型。结果 3个基因座分别检出6个、4个和4个等位基因,共38种单倍型,其单倍型的个体识别率为0.8796。结论 Y-STR基因座复合扩增体系和建立的Y染色体STR数据库,在法医学鉴定中有应用意义。     

Asia’s Security Triangles: Maritime Minilateralism in the Indo-Pacific

Since the early years of the twenty-first century, a number of key regional governments have consciously chosen to alter the way they talk about the region, and have now largely shifted from using the ‘Asia-Pacific’ to the ‘Indo-Pacific’ construct. But after three decades of utilising the ‘Asia-Pacific’ concept, why has this been the case and how might this shift in geographical conceptualisation alter the strategic framework of the region? This paper argues that the ‘Indo-Pacific’ is a regional reconceptualisation utilised by Japan, Australia, India and the USA to address deficiencies in Asia’s maritime security and institutional architecture, which are being simultaneously influenced by a more assertive Chinese posture and waning U.S. influence. Additionally, the Indo-Pacific concept has developed in tandem with a transformation in the regional security architecture. The utilisation of maritime minilateralism between Japan, India, Australia and the USA supplements Asia’s bilateral American alliances, with an array of trilateral security dialogues or ‘security triangles’. The Asia-Pacific to Indo-Pacific shift is really an instance of an emerging minilateral security regionalism, rather than the predominant forms of bilateral and multilateral security and economic regionalism that have dominated Asia in recent decades.     

Voting for Movement Parties in Southern Europe: The Role of Protest and Digital Information

In recent years ‘movement parties’ such as Syriza in Greece, the Movimento 5 Stelle in Italy, Podemos in Spain and—to a lesser extent—Bloco de Esquerda in Portugal shook national party systems, breaking the consolidated dynamics of political competition. Despite growing interest in movement parties, there has been scant attention to the role of citizens adopting unconventional forms of action and using digital media in accounting for their electoral performance. To fill this gap, four original internet-based post-electoral surveys are employed showing that protesters and digital media users are more likely to vote for these parties, despite important country differences.     

Back to the Suitcase? Emigration during the Great Recession in Spain

The complex and multidimensional economic crisis experienced by Spain since 2008 significantly altered migration patterns in this country. Large scale unemployment contributed to slow down migrant inflows and accelerated out-migration flows in Spain. The media coverage of these processes created a distorted image of the patterns of migration affecting Spain during the crisis. Although the incipient economic recovery has not had a major impact in terms of modifying the migration dynamics triggered by the crisis, the media attention to this issue has substantially decreased without questioning its previous approach to the phenomenon. This article presents extensive data from a wide range of sources covering the period 2008–2016 to extract detailed information about the reality of crisis-era migration flows in Spain, and discusses the extent to which the media treatment of the issue has contributed to a partial and misleading view of the causes and consequences of the new Spanish emigration.     

“哈尔斯坦主义”与西德外交(1955-1970年)

冷战时期曾一度影响西德对外关系、特别是与东欧邻国关系的"哈尔斯坦主义"(1955-1970年)一直尚未得到系统的研究。它在战后重建欧洲和平与安全中到底起到了哪些作用?波恩政府坚持这一原则时,阿登纳及其幕僚是否考虑到他们有限的能力与影响?实际上,由于在外交实践中陷入困境,"哈尔斯坦主义"到1963年就开始失去意义。将"哈尔斯坦主义"置于整个西德外交的视角下进行考察,可以发现,"哈尔斯坦主义"不仅未能实现西德外交的预期目标,而且也反映出德国人在外交理念上的传统风格,即在对现实政治目标的追求中带有焦躁与鲁莽的心态。     

对社会转型期加强中国共产党执政基础建设的认识与思考

我国进入社会转型期,人们的行为方式、生活方式和价值观念都发生了明显的变化。这些变化,给党的执政基础建设带来了广泛而深远的影响。针对这些影响,在社会转型时期,加强党的执政基础建设,要努力做好夯实党执政的意识形态基础文章,做好拓展党执政的绩效基础文章,做好构建党执政的法理基础文章,做好坚持执政为民和密切党群关系的文章。     

德国非营利组织:现状、特点与发展趋势

近年来,非营利组织成为德国政界和学界的中心议题之一,并被赋予了重塑(现代化)德国社会的重要使命.本文将在德国福利制度转型的宏观视野下,运用现有文献资料,梳理和介绍德国非营利组织的类型、结构体系、活动领域、收入结构、雇员及志愿者规模、劳动力市场份额等现状与特点,并就德国非营利组织的发展趋势做简要描述和分析.