Variability of the Entire Mitochondrial DNA Control Region in a Human Isolate from the Pas Valley (Northern Spain)

Abstract: In this study, we analyzed the entire mtDNA control region in 61 unrelated individuals from the Pas Valley (Cantabria), a human isolate from northern Spain, to evaluate the suitability of this analysis to increase the power of discrimination of this locus for forensic purposes in human isolates. Low values obtained for the diversity parameters confirmed the relative isolation of this human group. The main findings of this study indicated that even the analysis of the entire mtDNA control region may have important limitations for use in forensic casework when dealing with human isolates: none of the 44 individuals who exhibited identical HVI‐HVII haplotypes could be further differentiated by analysis of segment HVIII. Nevertheless, analysis of the entire mtDNA control region proved to be useful to determine the ancestry of the samples examined, by contributing to the confirmation, and, on occasion, even to the refinement of the haplogroup assignment.     

Population data of new Y-chromosome STRs GATA C4, DYS438, DYS437, GATA A7.2, GATA H4, DYS439 and GATA A10 in males from Colombia

Two hundred twenty-five unrelated males were typed for 7 over 8 loci Y-chromosome STRs proposed in a collaborative study by The Spanish and Portuguese ISFG Working Group. The markers amplification were in two multiplex reactions GEPY I with GATA C4, DYS438, DYS437, DYS461 (GATA A7.2) and GEPY II with GATA H4, DYS439, GATA A10 and DYS460 (GATA A7.1). All gene diversities were upper 0.5 with the highest value in DYS439 with 0.64. Furthermore, 152 haplotypes from 7 loci Y-chromosome STRs were found within studied population and a high haplotype diversity 0.9902 was found. The DYS460 (GATA A7.1) marker can not be studied because its diverse alleles were not able for interpret.