Adaptation and evaluation of the PrepFiler™ DNA extraction technology in an automated forensic DNA analysis process with emphasis on DNA yield, inhibitor removal and contamination security

Within the initial step of the forensic DNA analysis process, the DNA extraction efficiency and especially the removal of potential PCR inhibitors is crucial for subsequent steps, e.g. quantification by real-time PCR and amplification of short tandem repeats (STRs). The protocol of the PrepFiler™ Forensic DNA Extraction Kit was optimized for the application on a Tecan liquid handling workstation Freedom EVO^® 150. This modified application of the PrepFiler™ technology was compared with respect to DNA yield, sensitivity and the ability to remove potential PCR inhibitors to an established routine method working on the same liquid handling workstation based on ChargeSwitch^® Technology (CST) from Invitrogen.     

Forensic anthropology in the global investigation of humanitarian and human rights abuse: Perspective from the published record

Forensic anthropologists have played key roles in the historical development of forensic science applications to global humanitarian and human rights issues. These anthropological initiatives can be traced back to the Smithsonian seminar organized by T. D. Stewart in 1968 and published in 1970. Key developments include the 1984 delegation sent by the American Association for the Advancement of Science to Argentina and the formation of the Argentine Forensic Anthropology Team. Subsequent highlights include major anthropological involvement in support of investigations by international criminal tribunals, formation of forensic anthropology teams in different countries and activities of the International Commission of Missing Persons and the forensic unit of the International Committee of the Red Cross. Recent developments feature the formation of the Humanitarian and Human Rights Resource Center of the American Academy of Forensic Sciences and its support of worthwhile projects in many countries. The published record provides historical perspective on these developments.     

Is Sincerity the First Virtue of Social Institutions? Police,Universities, and Free Speech

In the final chapter of Speech Matters, Seana Shiffrin argues that institutions have especially stringent duties to protect speech freedoms. In this article, I develop a few lines of criticism. First, I question whether Shiffrin’s framework of justified suspended contexts is appropriate for institutional settings. Second, I challenge the presumption that the knowledge-gathering function performed by police is necessarily compromised by insincere practices. Third, I criticize Shiffrin’s characterization of the university as involving a complete repudiation of enforced consensus, and I express doubts about the close connection between education and democratic legitimation that Shiffrin endorses. Finally, I raise a problem with the book’s overall argument: even if one agrees that speech freedoms are necessary for moral development, they also may be threatening to moral development. The upshot is that the protection of speech should be modulated in order to account for the potential conflicts between sincerity and other valuable ends, rather than being oriented above all to sincerity.

     

Effects of Human Electro‐Muscular Incapacitation (HEMI) Devices on Cardiovascular Changes in Anesthetized Swine as Measured by Transesophageal Echocardiography (TEE)

The abundance of, and reliance upon, human electro‐muscular incapacitation (HEMI) devices, especially in law enforcement, has generated scrutiny and examination of these technologies. The purpose of this study was to examine cardiovascular effects resulting from typical (5 sec) and longer activation (20 sec) HEMI applications studying myocardial function and peripheral vascular system using a combination of invasive cardiovascular catheters and transesophageal echocardiography (TEE). Six healthy swine (Sus scrofa) 3–5 months in age and weighing between 60 and 86 kg were anesthetized and exposed to the TASER Model X26 waveform while transesophageal echocardiography was performed. Stroke volume was shown to statistically decrease during HEMI application indicating an increase in systemic vascular resistance, but HEMI application did not result in myocardial dysfunction (“cardiac stunning”).     

Ebstein Anomaly and Sudden Childhood Death

A 13‐year‐old girl is reported who died suddenly and unexpectedly in her sleep from previously undiagnosed Ebstein anomaly. At autopsy, there was dilatation of the right atrium with marked dilatation of the right auricle and apical displacement of the tricuspid valve into the right ventricular cavity with atrialization of the upper portion of the right ventricle. There were also prominent dysplastic changes in both the septal and posterior leaflets of the tricuspid valve with thickening of the valve and fusion of leaflets to the wall of the ventricle. Histology of the myocardium showed focal, minor microscopic areas of interstitial fibrosis with marked fibrous dysplasia and thickening of the tricuspid valve. Lethal arrhythmias occur in this condition because of the geographical relationship of the conduction system to the abnormal anatomical structures. As adolescents who died suddenly are often minimally symptomatic, cases will rarely present de novo to forensic autopsy.     

The Practice of Law With LGBT‐Parent Families

In this article, I examine how a history of legal conflict has produced a constantly evolving professional identity for lawyers representing lesbian/gay/bisexual/transgender (LGBT) clients on family matters. Drawing on in‐depth interviews with 21 lawyers, I describe variation across areas of specialization, advertising, clientele, and access to professional networks. In addition, I focus on how sociopolitical and legal context shapes professional identity and practice for these lawyers, demonstrating the importance of practice location for this group of lawyers. Although interviews were conducted prior to national marriage recognition, these findings provide insight into the future development of the LGBT family law profession post‐Obergefell.     

Economic Abuse Experiences and Depressive Symptoms among Victims of Intimate Partner Violence

There are significant, detrimental effects of physical, sexual, and psychological intimate partner violence (IPV) on victims’ mental health and well-being. However, little is known about the impact of economic abuse. To address this gap, the purpose of this study was to examine the association between economic abuse and depression and to explore whether the association between economic abuse and depression could be accounted for by other forms of IPV victimization (physical, sexual, and psychological abuse). Data from 457 female victims of IPV, recruited from 14 domestic violence programs across 10 states and Puerto Rico, were examined to explore the association between economic abuse and depressive symptoms. A series of hierarchical regressions were used to examine whether the addition of economic abuse improved the association between depression over and above participants’ sociodemographic characteristics and experiences of psychological, physical, and sexual IPV. The majority (93%) of participants reported experiencing economic abuse from their intimate partner. The findings from a series of multiple regression analyses revealed that economic abuse was uniquely associated with depression after accounting for other forms of IPV victimization and the sociodemographic characteristics of the participants. Implications include the need for screening, intervention, and prevention of economic abuse among IPV victims and continued research regarding economic abuse experiences.     

Shoot/No-Shoot Decisions in the Context of IED-Detection Training and Eyewitness Memory for Persons

Cognitive approaches to training for the detection of improvised explosive devices (IED’s) are of increasing importance. However, there is a question as to the degree to which such training might interfere with other important law enforcement (LE) functions in the field, and the degree to which such training might enhance other important cognitive/perceptual functions. A promising cognitive approach to IED training, the SMOKE system, was provided to respondents, who then responded to shoot/no-shoot decisions, important LE situations of increasing relevance. It was shown that SMOKE training did not interfere with shoot/no-shoot decisions. However, those with SMOKE training performed better than control respondents on eyewitness memory for the perpetrator of a given crime in field-valid scenes. This indicates that cognitively based training may enhance vigilance and resultant memory in field situations.     

The Anarchy of Responsibility: Alterity as a Vehicle for Self-Representation (An Ethical Obligation Demanded or Owed)

This paper explores who, in the Levinasian sense, is the ethical subject. Central to Levinas’s philosophy, is the priority he accords to the ethical encounter with the other that precedes all thought for, or of, oneself. It is in the face of the other (‘alterity’) that one discerns the source of all ethics; an ethics that obliterates all competing claims based on self interest because it eschews all demands for reciprocity. The face of the other commands us to respond unconditionally and in so doing provides the opportunity for a moral existence. Is to speak as the other an inversion of Levinas’s philosophy? Is not the authentic voice of alterity one that is obsessed with the needs of the other and not of the self, the obligations that the self owes to the other and not those owed in return. I argue that to become the voice of the other is to act unethically in a Levinasian sense and thus betray her otherness, whilst recognising that she is my other and it is my response to her that matters.     

A new psychometric instrument assessing vulnerability to risk of suicide and self-harm behaviour in offenders: Suicide Concerns for Offenders in Prison Environment (SCOPE)

This study aimed to develop a new psychometric instrument to assess vulnerability to risk of suicide and nonfatal self-harm behaviour in young adult male and female offenders. In total three studies were conducted to assess the psychometric properties of the new instrument using both exploratory and confirmatory factor analysis in different samples. Participants in all three studies included a total of 1,166 young adult offenders across six Her Majesty's Prisons. The new instrument, Suicide Concerns for Offenders in Prison Environment (SCOPE), contained 28 items scoring on two subscales. The factorial structure of the new instrument initially obtained with exploratory factor analysis was subsequently confirmed in a new sample. The internal consistency of the two subscales were acceptable but the test-retest reliability coefficients were moderate. Concurrent validation with the Beck Hopelessness Scale was acceptable and SCOPE showed the ability to discriminate between those at risk and those with no known history of attempted suicide and nonfatal self-harm behaviour ( p < 0.01).