全文获取类型
收费全文 | 976篇 |
免费 | 26篇 |
专业分类
各国政治 | 45篇 |
工人农民 | 158篇 |
世界政治 | 50篇 |
外交国际关系 | 51篇 |
法律 | 486篇 |
中国政治 | 14篇 |
政治理论 | 195篇 |
综合类 | 3篇 |
出版年
2021年 | 7篇 |
2020年 | 11篇 |
2019年 | 23篇 |
2018年 | 30篇 |
2017年 | 31篇 |
2016年 | 18篇 |
2015年 | 16篇 |
2014年 | 28篇 |
2013年 | 129篇 |
2012年 | 24篇 |
2011年 | 27篇 |
2010年 | 31篇 |
2009年 | 35篇 |
2008年 | 28篇 |
2007年 | 32篇 |
2006年 | 40篇 |
2005年 | 28篇 |
2004年 | 33篇 |
2003年 | 32篇 |
2002年 | 34篇 |
2001年 | 28篇 |
2000年 | 24篇 |
1999年 | 22篇 |
1998年 | 14篇 |
1997年 | 17篇 |
1996年 | 15篇 |
1995年 | 13篇 |
1994年 | 8篇 |
1993年 | 7篇 |
1992年 | 19篇 |
1991年 | 20篇 |
1990年 | 16篇 |
1989年 | 18篇 |
1988年 | 12篇 |
1987年 | 11篇 |
1986年 | 14篇 |
1985年 | 16篇 |
1984年 | 11篇 |
1983年 | 7篇 |
1982年 | 14篇 |
1981年 | 6篇 |
1980年 | 11篇 |
1979年 | 8篇 |
1978年 | 6篇 |
1977年 | 8篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1973年 | 5篇 |
1972年 | 2篇 |
1969年 | 3篇 |
排序方式: 共有1002条查询结果,搜索用时 46 毫秒
61.
62.
An ingestion of an unknown quantity of Harmomed (dothiepin and diazepam) capsules in a suicide is described. The authors report a new and fast method of analysing and determining the dothiepin concentration in postmortem specimens. Quantitation of dothiepin, and its metabolite desmethyldothiepin was performed by ethyl acetate extraction from alkalinized body fluids before GC-MS analysis. The analyses were performed without any complex sample clean-up steps and with little sample material. Postmortem concentrations of dothiepin, desmethyldothiepin, diazepam and desmethyldiazepam in body fluids are given. The proposed method is a rapid procedure for analysis in cases of deliberate poisoning with the antidepressant drug dothiepin. 相似文献
63.
In four forensic cases of unidentified skeletal remains investigated in the last year, we were able to attach three to missing persons. In one case we could show that the discovered bone sample did not fit to a missing child. The method for mitochondrial DNA analysis for the routine identification of skeletal remains was established in our institute by typing bone samples of defined age obtained from Frankfurt's cemetery. Reproducible results were obtained for bones up to 75 years old. For analysis the bone samples were pulverised to fine powder, decalcified and DNA was extracted. From the DNA we amplified a 404-bp fragment from HV-1 and a 379-bp fragment from HV-2 of the mtDNA control region. After sequencing of the PCR products, the results were compared to the Anderson reference sequence and to putative maternal relatives. 相似文献
64.
65.
Debates concerning the taxation of prostitution have occurred in taxation law and in feminist literature. This article will integrate the case of Polok v. C.E.C. [2002] E.W.H.C, 156; [2002] S.T.C. 361, within the feminist legal canon. The case is discussed in the context of the argument of the European doctrine of fiscal neutrality, which dictates that, regardless of legality as amongst member states, if an activity is levied to V.A.T. in one member state, V.A.T. should be levied on it in all member states. The doctrine of sovereignty accepts the possibility that the integrity of the V.A.T. system may be compromised by the levying of tax on illegal activities, in terms of the cooperation between tax and other aspects of the U.K.’s legal system. European law, feminist law, commodification and the marketplace are all considered within the context of these principles. The article also considers the place of Polok within standard feminist texts on prostitution. Different paradigms of prostitution define different aspects of prostitution as ‘problems’, and the article considers the implications within a feminist reconstruction of Polok of this. The article suggests that the challenge for a feminist analysis of Polok is to remain within the realm of European tax and competition law, and to render the perspective of the employees of the Polok taxpayers part of the substance of the deliberations of the case. 相似文献
66.
The recent House of Lords decision in Quintavalle v Human Fertilisation and Embryology Authority has raised difficult and complex issues regarding the extent to which embryo selection and reproductive technology can be used as a means of rectifying genetic disorders and treating critically ill children. This comment outlines the facts of Quintavalle and explores how the House of Lords approached the legal, ethical and policy issues that arose out of the Human Fertilisation and Embryology Authority's (UK) decision to allow reproductive and embryo technology to be used to produce a 'saviour sibling' whose tissue could be used to save the life of a critically ill child. Particular attention will be given to the implications of the decision in Quintavalle for Australian family and medical law and policy. As part of this focus, the comment explores the current Australian legislative and policy framework regarding the use of genetic and reproductive technology as a mechanism through which to assist critically ill siblings. It is argued that the present Australian framework would appear to impose significant limits on the medical uses of genetic technology and, in this context, would seem to reflect many of the principles that were articulated by the House of Lords in Quintavalle. 相似文献
67.
Schneider PM Bender K Mayr WR Parson W Hoste B Decorte R Cordonnier J Vanek D Morling N Karjalainen M Marie-Paule Carlotti C Sabatier M Hohoff C Schmitter H Pflug W Wenzel R Patzelt D Lessig R Dobrowolski P O'Donnell G Garafano L Dobosz M De Knijff P Mevag B Pawlowski R Gusmão L Conceicao Vide M Alonso Alonso A García Fernández O Sanz Nicolás P Kihlgreen A Bär W Meier V Teyssier A Coquoz R Brandt C Germann U Gill P Hallett J Greenhalgh M 《Forensic science international》2004,139(2-3):123-134
Degradation of human DNA extracted from forensic stains is, in most cases, the result of a natural process due to the exposure of the stain samples to the environment. Experiences with degraded DNA from casework samples show that every sample may exhibit different properties in this respect, and that it is difficult to systematically assess the performance of routinely used typing systems for the analysis of degraded DNA samples. Using a batch of artificially degraded DNA with an average fragment size of approx. 200 bp a collaborative exercise was carried out among 38 forensic laboratories from 17 European countries. The results were assessed according to correct allele detection, peak height and balance as well as the occurrence of artefacts. A number of common problems were identified based on these results such as strong peak imbalance in heterozygous genotypes for the larger short tandem repeat (STR) fragments after increased PCR cycle numbers, artefact signals and allelic drop-out. Based on the observations, strategies are discussed to overcome these problems. The strategies include careful balancing of the amount of template DNA and the PCR cycle numbers, the reaction volume and the amount of Taq polymerase. Furthermore, a careful evaluation of the results of the fragment analysis and of automated allele calling is necessary to identify the correct alleles and avoid artefacts. 相似文献
68.
Shewale JG Nasir H Schneida E Gross AM Budowle B Sinha SK 《Journal of forensic sciences》2004,49(6):1278-1290
The Y-PLEX 12 system, developed for use in human identification, enables simultaneous amplification of eleven polymorphic short tandem repeat (STR) loci, namely DYS392, DYS390, DYS385 a/b, DYS393, DYS389I, DYS391, DYS389II, DYS 19, DYS439 and DYS438, residing on the Y chromosome and Amelogenin. Amelogenin provides results for gender identification and serves as internal control for PCR. The validation studies were performed according to the DNA Advisory Board's (DAB) Quality Assurance Standards. The minimal sensitivity of the Y-PLEX 12 system was 0.1 ng of male DNA. The mean stutter values ranged between 3.76-15.72%. A full male profile was observed in mixture samples containing 0.5 ng of male DNA and up to 400 ng of female DNA. Amelogenin did not adversely affect the amplification of Y-STRs in mixture samples containing male and female DNA. The primers for the Y-STR loci present in Y-PLEX 12 are specific for human DNA and some higher primates. None of the primate samples tested provided a complete profile at all 11 Y-STR loci amplified with the Y-PLEX 12 system. Y-PLEX 12 is a sensitive, valid, reliable, and robust multiplex system for forensic analysis, and it can be used in human forensic and male lineage identification cases. 相似文献
69.
Balogh MK Burger J Bender K Schneider PM Alt KW 《Forensic science international》2003,137(2-3):188-195
A systematic study was conducted to investigate whether DNA can be successfully extracted from latent fingerprints deposited on ordinary paper and analysed using short tandem repeat profiling and mitochondrial DNA sequencing. In order to evaluate the performance of latent fingerprint analysis in a criminal case, experiments with varying conditions were carried out to improve our understanding of low copy number (LCN) DNA typing. After optimising the extraction methods to achieve increased sensitivity, the examination of touched paper can routinely yield the STR profile of the individual who has touched it. A fingerprint can therefore be considered as a potential source of DNA for genetic identification. Nevertheless, the findings of our "after enhancement experiment" (using chemically or physically pre-treated fingerprints), and our "mixture experiment" (using fingerprints from three to four people on the same sheet of paper) help to define the limitations of the low copy number PCR technique in forensic casework. 相似文献
70.
Leclair B Sgueglia JB Wojtowicz PC Juston AC Frégeau CJ Fourney RM 《Journal of forensic sciences》2003,48(5):1001-1013
Improvements in detection limits/sensitivity and lower sample consumption are potential benefits of reducing PCR reaction volumes used in forensic DNA typing of crime scene samples. This premise was studied first with experimental mixtures and a nine-loci megaplex, which demonstrated stochiometric amplification and accurate detection. Next, adjudicated casework samples were subjected to amplification under 15 different template DNA to PCR reaction volume ratios. Reduction of PCR reaction volume and DNA down to 10 microL and 0.500 ng, respectively, produced identical profiles with the same signal intensity and heterozygous allele peak height ratio (HR). Reduction to 5 microL and 0.063 ng yielded HR values that were slightly affected in one to three STR loci. PCR reaction volume reduction can enhance detection and sensitivity while reducing the consumption of irreplaceable crime scene samples. 相似文献