Validation of male-specific, 12-locus fluorescent short tandem repeat (STR) multiplex

Y chromosome-specific short tandem repeat (Y-STR) analysis has become another widely accepted tool for human identification. The PowerPlex Y System is a fluorescent multiplex that includes the 12 loci: DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. This panel of markers incorporates the 9-locus European minimal haplotype (EMH) loci recommended by the International Y-STR User Group and the 11-locus set recommended by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Described here are inter-laboratory results from 17 developmental validation studies of the PowerPlex Y System and include the following results: (a) samples distributed between laboratories and commercial standards produced expected and reproducible haplotypes; (b) use of common amplification and detection instruments were successfully demonstrated; (c) full profiles were obtained with standard 30 and 32 cycle amplification protocols and cycle number (24-28 cycles) could be modified to match different substrates (such as direct amplification of FTA paper); (d) complete profiles were observed with reaction volumes from 6.25 to 50 microL; (e) minimal impact was observed with variation of enzyme concentration; (f) full haplotypes were observed with 0.5-2x primer concentrations; however, relative yield between loci varied with concentration; (g) reduction of magnesium to 1mM (1.5 mM standard) resulted in minimal amplification, while only partial loss of yield was observed with 1.25 mM magnesium; (h) decreasing the annealing temperature by 2-4 degrees C did not generate artifacts or locus dropout and most laboratories observed full amplification with the annealing temperature increased by 2 degrees C and significant locus dropout with a 4 degrees C increase in annealing temperature; (i) amplification of individual loci with primers used in the multiplex produced the same alleles as observed with the multiplex amplification; (j) all laboratories observed full amplification with >or = 125 pg of male template with partial and/or complete profiles observed using 30-62.5 pg of DNA; (k) analysis of < or = 500 ng of female DNA did not yield amplification products; (l) the minor male component of a male/female mixture was observed with < or =1200-fold excess female DNA with the majority of alleles still observed with 10,000-fold excess female; (m) male/male mixtures produced full profiles from the minor contributor with 10-20-fold excess of the major contributor; (n) average stutter for each locus; (o) precision of sizing were determined; (p) human-specificity studies displayed amplification products only with some primate samples; and (q) reanalysis of 102 non-probative casework samples from 65 cases produced results consistent with original findings and in some instances additional identification of a minor male contributor to a male/female mixture was obtained. In general, the PowerPlex Y System was shown to have the sensitivity, specificity and reliability required for forensic DNA analysis.     

Correlates of Behavioral Outcomes in Sexually Abused Children

The present study attempted to identify individual and contextual factors associated with outcomes in a group of 63 latency-aged children disclosing sexual abuse (SA). Children reporting SA were found to display greater internalizing and externalizing behavioral difficulties as well as more sexualized behaviors relative to same-age non-abused peers. Mothers also reported these children as less socially competent than their peers. Family contextual factors appeared to be associated with behavioral difficulties and made a unique contribution to the prediction of externalizing and sexualized behaviors. Of the personal variables, avoidance coping was found to be linked to poorer outcomes. In examining possible factors linked to ‘resilient’ outcomes in a 6-month time-frame, family conflict and avoidance coping were found to be associated to clinical status in children reporting SA.     

Marriage at the intersection between tradition and globalization

In this paper, we will investigate the popularity of marriage migration between Turkish communities in Western Europe and emigration regions in Turkey. Our focus here is specifically on the Belgian case, namely the ‘Emirdag connection’. In Belgium, the majority of immigrants with a Turkish background come from the region of Emirdag, in the province of Afyon. On the basis of quantitative research methodologies, we first consider the magnitude of the phenomenon and the socio-economic situation of those involved. Using the qualitative research techniques of participant observation and in-depth interviews, we analyze the mechanisms in an attempt to explain marriage migration between these regions. Why do so many young people, born and raised in Western Europe, opt for an unknown partner from a region that is largely unknown to them but which proves to be their parents', or even grandparents', region of origin? Why does migration remain such a valuable life project for many young people in these regions of origin, despite the real danger of many negative side effects? The popularity of marriage migration is often explained by its role in making migration possible. However, migration theories alone cannot explain this phenomenon. Here we will argue that the existence of a ‘culture of migration’ that binds the region of origin with the region of destination and in which ‘the family’ as an institution is capable of building a bridge between traditional praxis, as well as the challenges linked to international migration, are crucial for understanding the enduring popularity of marriage migration.     

The Role of Ego-Resiliency as Mediator of the Longitudinal Relationship between Family Socio-Economic Status and School Grades

Family socio-economic status (SES) represents one of the major determinants of youth’s scholastic achievement, and thus it is important to unravel the psychological factors underlining this relation. In this article, we examined youth’s ability to flexibly adapt and, thus, cope with harsh environmental conditions—assessed by the construct of ego-resiliency—as a mediating mechanism in the across-time association between family SES and academic achievement. The longitudinal sample was composed of 265 (56% females) Italian students who were about 13 years old at Time 1 (T1) and about 18 years old at Time 2 (T2). In a structural equation model analysis, family SES significantly predicted ego-resiliency 6 years later while controlling for the latter’s strong longitudinal stability. Students’ school grades at the end of senior high school were also predicted by ego-resiliency assessed at the age of 13, controlling for grades in the last year of junior high school, gender, and initial differences in ages. In accordance with the posited hypothesis, this study provided support for a two-wave meditational model in which the relation between family SES at 13 years and later school grades at 19 years was mediated by ego-resiliency. All in all, results support the argument that being resilient, and thus being able to flexibly adapt one’s own emotional state and resultant behavior, matters to school success.     

Analysing Choice in Australian Individual Funding Disability Policies

The Australian National Disability Insurance Scheme (NDIS) will allocate funding packages to people with disability who are assessed as needing paid support. The NDIS is an example of individual funding, which is currently not the dominant way of organising disability support in Australia. Individual funding aims to increase opportunities for personal choice. We present a framework for understanding current individual funding policies in each Australian jurisdiction according to two policy dimensions that potentially enable greater personal choice for people with disability: who holds their allocated funds and where support can be purchased. The findings show wide disparities in choice across the country, particularly due to constrained funds and the shortage of support to purchase in regional areas. The analysis demonstrates that NDIS implementation will need to consider that, while individual funding can be empowering for some people with disability, enabling choice can be challenging for administrators and service providers.     

Security and discourse: the Israeli–Palestinian water conflict

When conflictive viewpoints are discursively strengthened, they develop into a ‘conflict discourse’ with a specific discursive structure which perpetuates conflict, like the discursive securitisation of an issue for varying audiences. When they are weakened, however, societal discourse can potentially change so that agreement becomes possible again, thus achieving discursive conflict transformation. This article analyses the Israeli and the Palestinian water discourse. On both sides, the dominant discourse structures underscore the conflictive issues regarding the distribution of water between Israelis and Palestinians, thus making communication, let alone negotiation, downright impossible. While Palestinians regard the natural water resources as sufficient in principle and the existing scarcity as entirely politically induced, Israelis perceive the natural water resources as absolutely scarce while receiving major de-securitisation impulses from the possibility of desalination. In the respective (minor) counter-discourses, however, possible starting points for dialogue and conflict resolution are visible.