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141.
利用差减抑制杂交(SSH)技术,以旋毛虫5日龄成虫的cDNA为试验方(tester),以肌幼虫+3日龄成虫的cDNA为驱动方(driver),制备5日龄成虫差减cDNA,并与pT-Adv载体相连接,转入大肠埃希氏菌TOP 10F.以试验方的差减cDNA PCR产物+未差减cDNA为试验方探针,以驱动方的差减cDNA PCR产物+未差减cDNA为驱动方探针,对5日龄成虫差减cDNA克隆进行初步筛选,对筛选到的阳性克隆进一步用southern blot进行鉴定,并对其测序,进行序列分析.结果表明,获得1个大小为464 bp的旋毛虫5日龄成虫期特异性cDNA片段,经BLAST软件分析表明,该序列是1个未见报道的旋毛虫基因序列片段,可能编码1种表皮胶原蛋白.这为旋毛虫5日龄成虫期特异性基因全长序列的钓取、分析及鉴定奠定了基础. 相似文献
142.
应用RT-PCR和nPCR扩增由4株甘肃省近期(1997-1998)猪瘟流行野毒株的E2基因,将其克隆到pGEMTEasy载体上,经转化、筛选、鉴定后,测出核苷核序列.4株流行毒株的E2基因核苷酸序列同源性为89.2%-99.7%,相应的氨基酸序列同源性为93.8%-99.0%.这4株流行毒株;与C-株(疫苗种毒)E2基因的核苷酸序列同源性为82.2%-84.3%,相应的氨基酸序列同源性为87.9%-90.2%,表明近期猪瘟流行毒株与C-株的gp55蛋白之间存在一定的差异. 相似文献
143.
144.
司法推理中应用的基本法律概念(下) 总被引:1,自引:0,他引:1
权利和义务上面已经隐约提示,“权利”一词容易被不加区别地使用,涵盖特定情形中的特权、权力或者豁免权,而不是最严格意义上的权利;一些权威判例也偶尔意识到了该用法的不严谨。法官斯特朗(Strong)在People v.Dikeman一案〔27〕中说:“权利”一词被词典编撰者定义为指称——— 相似文献
145.
近来,在许多俄罗斯人的社会意识中出现了一种为斯大林主义正名,甚至颂扬斯大林主义的倾向.这是对破坏性的后苏联改革的反应所引起的.苏联的成就,如国家工业化、科学文化的发展、居民的社会保障(全民就业、免费教育和医疗、实际上的免费住房、一个月的休假等),都被重新归功于斯大林,更不用说伟大的卫国战争的胜利了. 相似文献
146.
The goals of the Postvention Taskforce of the International Association for Suicide Prevention (IASP) are to increase awareness of postvention and survivor issues. One of the strategies is to organize meetings. We report on the 1st International Suicide Postvention Seminar, held as a 1-day preevent at the 11th European Symposium on Suicide and Suicidal Behavior in Portoroz, Slovenia, September 2006. The seminar provided a state of the art review of the postvention field, with plenary presentations on research, psychotherapeutic care, development of national networks, and of postvention materials. We summarize the presentations below. 相似文献
147.
Body mass has been neglected as a possible predictor of suicidal behavior. The present investigation explores the association between body mass index and completed suicide. Data are from the National Mortality Followback Survey and refer to 373 suicides, and 518 deaths from motor vehicle accidents as controls. The results of a logistic regression analysis indicated that, for females, the greater the BMI the greater the suicide risk. However, this relationship did not hold up under controls for race and other covariates of BMI. For males, the BMI was not significantly related to suicide risk either at the bivariate or multivariate levels of analysis. The best predictors of suicide were living alone for females, and alcohol abuse for males. 相似文献
148.
149.
My brand new bicycle had been stolen! Disbelief. Anger! Disappointment... I had been riding it for only a day,and was as excited as a,well,a boy with a new bike. Even though I only paid what would be the equivalent of half a tank of gas for my truck back home,I was still sorely put out. 相似文献
150.
Shulman S Zlotnik A Shachar-Shapira L Connolly J Bohr Y 《Journal of youth and adolescence》2012,41(5):593-606
This study examined the links between parental divorce, quality of maternal parenting, spousal relationships and middle adolescent
romantic competence in 80 mother-adolescent daughter pairs (40 divorced). Mothers were asked to describe their attitudes and
behaviors with regard to their daughters’ romantic behavior. In addition, mothers were interviewed about their own romantic
experiences when they were at the age of their daughters. Adolescent girls (mean age = 16.98 years; range 16–18) were administered
a comprehensive interview about romantic competence. Findings indicated that adolescent girls from divorced families showed
lower levels of romantic competence, which were expressed in their behavior, attitudes toward relationships and skill in handling
those relationships. Divorce was found to have had an adverse effect on girls’ romantic competence, whereas continued adaptive
parenting and spousal relationships alleviated the effect of divorce. Mothers’ coherent representation of their own adolescent
romantic experiences also alleviated the effect of divorce on daughters’ romantic behavior. Results show the important role
of family relationships in fostering romantic competence among adolescent girls. 相似文献