用引物Y_3,Y_4和DNA扩增技术作血液(痕)和毛根性别鉴定的研究

作者用引物Y_3、Y_4和DNA聚合酶链式反应(PCR)作微量人类血液(痕)和毛根的性别鉴定。扩增的靶序列位于Y染色体DNA特异3.4kb重复序列中,扩增产物为460bp。检材用量为:新鲜血液0.5μl、血痕纱纤维1mm、毛根单个。20例保存4个月的血痕与2例保存6年半的血痕性别判定结果均正确,无性别记载的保存9～11年的3例血痕显现了清晰的460bpY特异DNA扩增带。15例保存20天的自然脱落毛根性别判定结果均正确。本法省略了检材处理中的酚-氯仿抽提DNA等纯化步骤,既简化了实验操作,又减少了检验过程中外源DNA的污染机会和样品DNA的损耗,使这一性别鉴定方法更符合法医学实践的需要。     

THE LOGISTICS OF MINISTERIAL RESHUFFLES

Ministerial reshuffles are complex exercises requiring careful handling. A frequent occurrence in British government, their significance is usually seen in political terms. Prime Ministers attach great importance to maximizing the political advantages to be gained from them. This factor is the chief determinant of their logistics, the principal characteristics of which are secrecy in advance and the speed with which ministerial changes are executed. These features - as much as the frequency of ministerial changes per se - may disrupt the policy process and have serious implications for minister-civil servant power relations. Such problems could be alleviated by giving ministers advance notice of changes of post. The institution of a process of ministerial handovers would strengthen the position of incoming ministers by making them less dependent on their officials, upon whom they rely heavily for initial briefing at present.     

Psychology and law courses

During the past decade the field of psychology of law has changed considerably. Accompanying the growth of research and practice, there has been an increase in offerings of courses in this field. The courses are offered at both undergraduate and graduate levels and cover a wide spectrum of interests. The types of courses currently being offered and the student samples enrolling in the courses are examined. In addition, a review of the major textbooks used in the courses is provided.