Multivariate analysis for estimating the age of a bloodstain

Our objective is to provide crime laboratories with a technique for estimating the age of a bloodstain. Toward that goal, we have used multiplexed, real-time RT-PCR (or qPCR) to determine the relative stability of different-sized segments of the same RNA species as well as differences in stability between two different RNAs' change over time in bloodstains. Our results indicate that a multivariate analysis of the changing ratio of the different RNA segments can be used to differentiate between samples of different ages in the defined population. Bloodstains from 29 of 30 donors could be partitioned into different ages using this technique. Although further improvements will be required before this approach can be implemented in crime laboratories, the multivariate analysis holds promise of providing a reliable approach for temporally linking a bloodstain to the commission of a crime or excluding a bloodstain as being irrelevant to the case in question.     

Volunteering and the state

Volunteering participation rates vary greatly across countries even when the potential volunteers’ individual characteristics are controlled. We therefore analyze the volunteers’ motives against the backdrop of the predominant political and economic environment in different countries, focusing on the state’s role in influencing an individual’s decision to volunteer. Combining individual-level data from the European and World Values Survey with macroeconomic and political variables for OECD countries, we identify three channels through which governmental activities influence voluntary labor: size of the state (i.e., amount of public social expenditure), political consensus between voters and the government, and government support for democratization.     

Performance of the Emit® II Plus 6-Acetylmorphine Assay on the Viva-E® analyzer

We evaluated the performance of Emit(?) II Plus 6-Acetylmorphine Assay for human urine screening on the Viva-E(?) analyzer. Precision was evaluated using the cutoff and ±25% controls. Recovery and linearity were studied by spiking 6-acetylmorphine (6-AM) into human urine pools. Accuracy was evaluated using urine specimens and the results were compared to those from GC/MS. Cross-reactivity with structurally related drugs was assessed at different cross-reactant concentrations. Interferences were assessed in the presence of 7.5 and 12.5 ng/mL of 6-AM. The qualitative repeatability coefficients of variation (CV's) ranged from 0.3% to 0.4% and the within-lab CV's ranged from 2.0% to 2.2%. In analyte units (ng/mL), the repeatability CV's ranged from 1.3% to 2.2% and the within-lab CV's ranged from 2.6% to 4.3%. The limit of detection of the assay was found to be 2.1 ng/mL. Recovery was within 15% of expected value. Linearity was 2.1-20 ng/mL. Method comparison showed 99% agreement with GC/MS. The assay had minimal cross-reactivity with morphine, morphine-3-glucuronide, morphine-6-glucuronide and other opioids. No interference was observed with endogenous interferences and structurally unrelated drugs. The assay correctly classified CAP survey samples. The Emit(?) II Plus 6-Acetylmorphine Assay will be a suitable screening method for urine specimens in both qualitative and semi-quantitative analyses.     

Tattoo frequency and types among homicides and other deaths, 2007-2008: a matched case-control study

An estimated 25% of the US population aged 18 to 50 years has a tattoo, which have been associated with markers of high-risk behaviors including alcohol and drug use, violence, carrying weapons, sexual activity, eating disorders, and suicide. This study compares tattoo prevalence and type in a homicide population to those of an age-, race-, and sex-matched control group of nonhomicide deaths. The data for this study were abstracted from autopsy records maintained by the Jefferson County Alabama Coroner/Medical Examiner's Office for the years 2007 and 2008. Odds ratios and 95% confidence intervals for the association between homicide and tattoo presence and characteristics were calculated using conditional logistic regression. There was no association between tattoo presence and death by homicide; however, among blacks, memorial tattoos were significantly more common among homicides compared with other types of deaths (odds ratio, 2.50; 95% confidence interval, 1.10-5.68). The results of the current study suggest that specific types of tattoos, but not all tattoos, may be risk factors for homicide. Other factors, such as race and lifestyle, along with tattoos may need to be considered.     

Adolescent egocentrism: Exploration into possible contributions of parent-child relations

A family relations model for the study of adolescent egocentrism was tested in an exploratory study of the relationship between parental socialization styles and adolescents' imaginary audience behavior. A sample of adolescent boys (n=58) and girls (n=57) responded to Heilbrun's Parent-Child Interaction Rating Scale and Schaefer's Parent-Behavior Inventory and completed Elkind and Bowen's Imaginary Audience Scale. As hypothesized, rejection-control was associated with increased imaginary audience behavior, while physical affect was negatively related to self-consciousness. Sex differences were noted, with rejection-control being most important in predicting self-consciousness for boys and physical affect being the best predictor of girls' egocentrism behavior. The data provide an alternative model to a cognitive developmental perspective of adolescent egocentrism development.Research was partially supported through the Western Regional Research Project W-144, Development of Social Competency in Children, with funding in part from the Science and Education Administration/Cooperative Research of USDA, and the Utah State University Agricultural Experiment Station.Received his M.A. in psychology from the University of Nebraska at Omaha and Ph.D. in human development from the Pennsylvania State University. Current research interest is personality and social development of children and adolescents.Completed his M.S. Degree in family and human development at Utah State University. Current research interests include the study of interpersonal perception and attraction and human socialization.     

Adolescent life changes and illness: A comparison of three models

This article examines how stressful life-events relate to the occurrence of illness in a nonclinical random sample of adolescents and what processes intervene in this relationship. Three alternative models of the life events and illness relationship are compared. Data support a “direct stress and illness model” as opposed to either a “sick role behavior model” or a “mental health model.” Social support from parents appears to mediate the life events-illness relationship. Undesirable and ambiguous life events have more impact on illness than desirable events do. Results are discussed in terms of adolescence as a unique developmental stage.