Headspace solid-phase microextraction (HS-SPME) and liquid-liquid extraction (LLE): comparison of the performance in classification of ecstasy tablets. Part 2

Headspace solid-phase microextraction (HS-SPME) is assessed as an alternative to liquid-liquid extraction (LLE) currently used for 3,4-methylenedioxymethampethamine (MDMA) profiling. Both methods were compared evaluating their performance in discriminating and classifying samples. For this purpose 62 different seizures were analysed using both extraction techniques followed by gas chromatography-mass spectroscopy (GC-MS). A previously validated method provided data for HS-SPME, whereas LLE data were collected applying a harmonized methodology developed and used in the European project CHAMP. After suitable pre-treatment, similarities between sample pairs were studied using the Pearson correlation. Both methods enable to distinguish between samples coming from the same pre-tabletting batches and samples coming from different pre-tabletting batches. This finding emphasizes the use of HS-SPME as an effective alternative to LLE, with additional advantages such as sample preparation and a solvent-free process.     

Immediate anaphylactic death following antibiotics injection: splenic eosinophilia easily revealed by pagoda red stain

The comparison of 4 cases of immediate anaphylactic death following the intramuscular injection of antibiotics (different types of penicillin or cephalosporins) with 4 cases of immediate non-anaphylactic death (induced by different causes) recognized splenic eosinophilia, as the main feature for the differential diagnosis, in agreement with isolated previous studies. The use of a stain (pagoda red) little known and seldom employed in Pathology and in Forensic Medicine, showed the concomitant massive presence (immunohistochemically confirmed) of mast cells and degranulated mast cells, the latter mainly located in splenic sinuses. The whole of our findings led us to consider the spleen as the possible shock organ in man. Waiting for further judgment on our hypothesis, we seized the opportunity to remark the employment of the pagoda red stain when the contemporary demonstration of eosinophils, mast cells and degranulated mast cells is required.     

Gasoline on hands: preliminary study on collection and persistence

The identification of an arsonist remains one of the most difficult challenges a fire investigation has to face. Seeking and detection of traces of gasoline could provide a valuable information to link a suspect with an arson scene where gasoline was used to set-up the fire. In this perspective, a first study was undertaken to evaluate a simple, fast and efficient method for collecting gasoline from hands, and to assess its persistence over time. Four collection means were tested: PVC, PE and Latex gloves, as well as humidified filter paper. A statistical assessment of the results indicates that Latex and PVC gloves worn for about 20 min, as well as paper filter rubbed on hands, allow an efficient collection of gasoline applied to hands. Due to ease of manipulation and to a reduced amount of volatile compounds detected from the matrix, PVC gloves were selected for the second set of experiments. The evaluation of the persistence of gasoline on hands was then carried out using two initial quantities (500 and 1000 microl). Collection was made with PVC gloves after 0, 30 min, 1, 2 and 4h, on different volunteers. The results show a common tendency of massive evaporation of gasoline during the first 30 min: a continued but non-linear decrease was observed along different time intervals. The results of this preliminary study are in agreement with other previous researches conducted on the detection of flammable liquid residues on clothes, shoes and skin.     

‘Provincializing’ critical theory: Islam,Sikhism and international relations theory

This article will attempt to ‘provincialize’ or ‘decentre’ critical theory by looking at the development of critical discourses from within the Islamic and Sikh religious traditions. Although important theological, philosophical and historical differences exist between the two communities, Islamic and Sikh narratives share a rejection of the subordination of the religious to the political and thus potentially challenge the Westphalian order. However, in the case of the Sikh Qaum, no clear distinction between ‘nation’ and ‘religion’ is possible given the strong attachment to a territorially defined ancestral homeland. This article suggests that both critical Islamic and Sikh discourses, particularly those emanating from the diaspora, are potentially compatible with the ‘discourse ethics’ of critical theory. This is, however, conditional on the recognition of the universality of their beliefs, a position incompatible with the ‘thin’ cosmopolitanism of critical theory.     

Bypassing a Centralized State: The Case of the Greek Subnational Mobilization in the European Union

For over 25 years successive administrative reforms have taken place in Greece, with a view to modernizing the subnational institutions. The European pressures come across as an influential factor inducing the process of change. But what has been the subnational responsiveness related to the rationalization initiatives? The aim of this paper is to investigate the Greek subnational authorities’ mobilization in the European arena, providing evidence of their bypassing the central state from a multi-level governance perspective. Building on the division between ‘financial' and ‘regulatory' mobilization, empirical data are drawn from the EU's environmental policy financial mechanism and the subnational representation and networking in Brussels. The figures show that only a few Greek subnational authorities financially mobilize; the vast majority show inertia. Moreover, their regulatory mobilization is hardly evident. It is argued that although the EU offers substantial incentives for mobilizing, domestic institutional capacity also accounts for much variation.     

When the slaves go marching out: indignatio,invisible bodies,and political theory

In January 2010, hundreds of illegal migrants took to the streets of Rosarno in Italy for a violent protest against the acts of racism which they had routinely suffered. A collective subject, considered invisible, dared to revolt. These migrants are an anomaly in the social, legal, and political senses. Their revolt is an example of rebellions who constitute a litmus test for the discourse of citizenship; it reveals itself as a form of political subjectivity and highlights the corporeality of the conflict. Understanding the revolt also troubles the boundary between body discourses and traditional political theory. In this paper, I analyse the revolt through categories of contemporary political theory such as the ‘bare life’ of Giorgio Agamben, and the ‘disagreement’ of Jacques Rancière. I show how these categories only partially help to interpret the phenomenon of this uprising. However, the Spinozist concept of indignatio is a more useful intellectual tool to interpret and understand the phenomenon of the revolt of Rosarno.     

GABA Receptors,Alcohol Dependence and Criminal Behavior

The aim of this study was to analyze the connection between alcohol dependence and criminal behavior by an integrated genetic–environmental approach. The research, structured as a case–control study, examined 186 alcohol‐dependent males; group 1 (N = 47 convicted subjects) was compared with group 2 (N = 139 no previous criminal records). Genetic results were innovative, highlighting differences in genotype distribution (p = 0.0067) in group 1 for single‐nucleotide polymorphism rs 3780428, located in the intronic region of subunit 2 of the GABA B receptor gene (GABBR2). Some environmental factors (e.g., grade repetition) were associated with criminal behavior; others (e.g., attendance at Alcoholics Anonymous) were inversely related to convictions. The concomitant presence of the genetic and environmental factors found to be associated with the condition of alcohol‐dependent inmate showed a 4‐fold increase in the risk of antisocial behavior. The results need to be replicated on a larger population to develop new preventive and therapeutic proposals.