Fatty Acid Profiles for Differentiating Growth Medium Formulations Used to Culture Bacillus cereus T‐strain Spores

Microbial biomarkers that indicate aspects of an organism's growth conditions are important targets of forensic research. In this study, we examined fatty acid composition as a signature for the types of complex nutrients in the culturing medium. Bacillus cereus T‐strain spores were grown in medium formulations supplemented with one of the following: peptone (meat protein), tryptone (casein protein), soy protein, and brain–heart infusion. Cellular biomass was profiled with fatty acid methyl ester (FAME) analysis. Results showed peptone cultures produced spores enriched in straight‐chained lipids. Tryptone cultures produced spores enriched in branched‐odd lipids when compared with peptone, soy, and brain–heart formulations. The observed FAME variation was used to construct a set of discriminant functions that could help identify the nutrients in a culturing recipe for an unknown spore sample. Blinded classification tests were most successful for spores grown on media containing peptone and tryptone, showing 88% and 100% correct identification, respectively.     

Involving the Public: When Are Surveys and Stakeholder Interviews Effective?1

Scholars and practitioners alike advocate involving stakeholders in environmental decision making, although there is uncertainty regarding the effectiveness of public involvement tools and the degree of public involvement in the decision making process. Some researchers have gone a step further to promote the use of public surveys and stakeholder interviews as preferred means to include public concerns in environmental decision making. However, there is little evidence as to whether public involvement tools are effective at representing public preferences, especially when there is a shortage of technical information to inform public opinion. This study examines the effectiveness of surveys and stakeholder interviews for assessing the District of Columbia's environmental problems in a comparative risk assessment. The findings suggest that these public involvement tools are less effective when there is a shortage of technical data. Instead, more deliberative forms of public involvement may generate greater convergence of opinion regarding environmental problems.     

DART‐MS as a Preliminary Screening Method for “Herbal Incense”: Chemical Analysis of Synthetic Cannabinoids

Direct analysis in real time mass spectrometry (DART‐MS) served as a method for rapid high‐throughput screening of six commercially available “Spice” products, detecting various combinations of five synthetic cannabinoids. Direct analysis in real time is an ambient ionization process that, along with high mass accuracy time‐of‐flight (TOF)‐MS to 0.0001 Da, was employed to establish the presence of cannabinoids. Mass spectra were acquired by simply suspending a small portion of sample between the ion source and the mass spectrometer inlet. The ability to test minute amounts of sample is a major advantage when very limited amounts of evidentiary material are available. In addition, reports are widespread regarding the testing backlogs that now exist because of the large influx of designer drugs. This method circumvents time‐consuming sample extraction, derivatization, chromatographic, and other sample preparative steps required for analysis by more conventional mass spectrometric methods. Accordingly, the synthetic cannabinoids AM‐2201, JWH‐122, JWH‐203, JWH‐210, and RCS‐4 were identified in commercially available herbal Spice products, singly and in tandem, at concentrations within the range of 4–141 mg/g of material. Direct analysis in real time mass spectrometry decreases the time necessary to triage analytical evidence, and therefore, it has the potential to contribute to backlog reduction and more timely criminal prosecution.     

Elimination of Bioweapons Agents from Forensic Samples During Extraction of Human DNA

Collection of DNA for genetic profiling is a powerful means for the identification of individuals responsible for crimes and terrorist acts. Biologic hazards, such as bacteria, endospores, toxins, and viruses, could contaminate sites of terrorist activities and thus could be present in samples collected for profiling. The fate of these hazards during DNA isolation has not been thoroughly examined. Our goals were to determine whether the DNA extraction process used by the Royal Canadian Mounted Police eliminates or neutralizes these agents and if not, to establish methods that render samples safe without compromising the human DNA. Our results show that bacteria, viruses, and toxins were reduced to undetectable levels during DNA extraction, but endospores remained viable. Filtration of samples after DNA isolation eliminated viable spores from the samples but left DNA intact. We also demonstrated that contamination of samples with some bacteria, endospores, and toxins for longer than 1 h compromised the ability to complete genetic profiling.     

Complex linkages between forced labor slavery and environmental decline in marine fisheries

Recent media attention on human rights abuses in the fishing sector, precipitated by undercover investigations from nongovernmental organizations and investigative journalists (e.g., Environmental Justice Foundation [EJF] 2014 ENVIRONMENTAL JUSTICE FOUNDATION (EJF). (2014) Slavery at Sea: The Continued Plight of Trafficked Migrants in Thailand’s Fishing Industry (London, UK: EJF). [Google Scholar], 2015a Environmental Justice Foundation. (2015a) Thailand’s Seafood Slaves: Human Trafficking, Slavery and Murder in Kantang’s Fishing Industry (London, UK: EJF). [Google Scholar], 2015b EJF. (2015b) Pirates and Slaves: How Overfishing in Thailand Fuels Human Trafficking and the Plundering of our Oceans (London, UK: EJF). [Google Scholar]; Mendoza, McDowell, Mason, and Htusan 2016 MENDOZA, Martha, MCDOWELL, Robin, MASON, Margie, HTUSAN, Esther. (2016) Fisherman Slaves: Human Trafficking and the Seafood We Eat (New York: AP Editions). [Google Scholar]), has prompted calls from the scientific community for increased transdisciplinary and empirical research of fisheries’ social dimensions, such as labor (Kittinger et al. 2017 KITTINGER, John N., TEH, Lydia C. L., ALLISON, Edward H., BENNETT, Nathan J., CROWDER, Larry B., FINKBEINER, Elena M., HICKS, Christina, SCARTON, Cheryl G., NAKAMURA, Katrina, OTA, Yoshitaka, YOUNG, Jhana, ALIFANO, Aurora, APEL, Ashley, ARBIB, Allison, BISHOP, Lori, BOYLE, Mariah, CISNEROS-MONTEMAYOR, Andrés M., HUNTER, Philip, LE CORNU, Elodie, LEVINE, Max, JONES, Richard S., KOEHN, Zachary, MARSCHKE, Melissa, MASON, Julia G., MICHELI, Fiorenza, McCLENACHAN, Loren, OPAL, Charlotte, PEACEY, Jonathan, PECKHAM, S. Hoyt, SCHEMMEL, Eva, SOLIS-RIVERA, Vivienne, SWARTZ, Wilf, and WILHELM, T.’Aulani. (2017) Committing to socially responsible seafood. Science, 356(6341), 912–913.[Crossref], [PubMed], [Web of Science ®] , [Google Scholar]). Given views that social and ecological systems are interdependent (Ostrom 2009 OSTROM, Elinor. (2009) A general framework for analyzing sustainability of social-ecological systems. Science, 325(5939), 419–422. [Crossref], [PubMed], [Web of Science ®] , [Google Scholar]), the need for theory development to explicate pathways for how this interdependence occurs and the potential for using policy and practices for intervention and prevention exist. Integrating ecological data and economics and human rights theory, Brashares and colleagues’ (2014) wildlife decline and social conflict framework offered a hypothesis about the negative association between fish stock declines and child slavery. Yet, more precision in terminology, pathways, and feedbacks may be warranted. With the aim of exploring empirical, conceptual, and theoretical support for Brashares et al.’s (2014 BRASHARES, Justin S., ABRAHMS, Briana, FIORELLA, Kathryn J., GOLDEN, Christopher D., HOJNOWSKI, Cheryl E., MARSH, Ryan A., McCAULEY, Douglas J., NUÑEZ, Tristan A., SETO, Katherine, and WITHEY, Lauren (2014) Wildlife decline and social conflict. Science, 345(6195), 376–378.[Crossref], [PubMed], [Web of Science ®] , [Google Scholar]) pathways, the revised theory developed in this article posits how forced labor slavery and environmental decline in marine fisheries may be linked.     

Gender Stereotypes and the Policy Priorities of Women in Congress

Political Behavior - Scholars find that women who run for Congress are just as likely to win as men are, yet women face considerable challenges related to their sex on the campaign trail. Women are...     

Loners,Colleagues, or Peers? Assessing the Social Organization of Radicalization

This study explores the utility of a sociological model of social organization developed by Best and Luckenbill (1994) to classify the radicalization processes of terrorists (i.e., extremist perpetrators who engaged in ideologically motivated acts of violence) who are usually categorized as loner or lone wolf attackers. There are several organizational frameworks used to define or classify violent acts performed by individuals who may or may not have ties to extremist groups, but these studies largely ignore the role of social relationships in radicalization and the extent to which they inform our knowledge of terror. To address this gap, we apply the Best and Luckenbill model of social organization using a qualitative analysis of three case studies of four lone actor or small cell terrorists. The findings demonstrate lone actors are not always true loners in the context of radicalization, and highlights the ways that the Internet and social ties foster the radicalization processes of terror.

     

Extending the Eros Effect: Sentience,Reality, and Emanation

This article provides three theoretical extensions to George Katsiaficas's understanding of the “eros effect,” which is a concept for explaining mass political awakenings and spontaneous rebellions. Katsiaficas argues that moments of mass revolt ignite the human need for justice and universal solidarity. That solidarity then radiates outward, alluring people into revolt and creating a groundswell of radicalism. Katsiaficas's heuristic is used here as a springboard for further theorizing. To begin, the eros effect is grounded in the sentient body—that is, the body's ability to feel itself and the world in ways that precede and exceed not only perception and language, but any and all oppressive conditions. It is also argued that the sentient body is a reality-creating organism that is inhibited and/or hindered by various oppressive conditions. This tension between creation and oppression produces the conditions of mass revolt. Lastly, the phenomenon of bodily emanation is introduced to explain the magnetic allure of mass rebellion—spontaneous mass revolts emanate a tangible and experiential feeling that attracts people to radical activity. Overall, this philosophy of sentience, reality, and emanation provides a sympathetic extension of Katsiaficas's eros effect.     

Indigenous Migration Dynamics in the Ecuadorian Amazon: A Longitudinal and Hierarchical Analysis

Amazonian indigenous populations are approaching a critical stage in their history in which increasing education and market integration, rapid population growth and degradation of natural resources threaten the survival of their traditions and livelihoods. A topic that has hardly been touched upon in this context is migration and population mobility. We address this by analysing a unique longitudinal dataset from the Ecuadorian Amazon on the spatial mobility of five indigenous groups and mestizo co-residents. Analyses reveal traditional and new forms of population mobility and migrant selectivity, including gendered forms of marriage migration and rural-urban moves driven by education. These results illustrate a dynamic present and an uncertain future for indigenous populations in which rural, natural-resource-based lifeways may well be sustained but with increasing links to urban areas.