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11.
Efficient capillary electrophoretic STR analysis requires rapid, reproducible and robust separation of DNA fragments with reasonable capillary longevity--this is currently accomplished using proprietary commercial polymeric sieving matrices specifically developed for this separation. These matrices, while effective, are costly and do not provide adequate resolution of STR DNA fragments in capillaries with shorter effective separation lengths, increasing the time required to accomplish the separation and minimizing the potential extrapolation to other miniaturized platforms. As the forensic community looks toward next generation microchip technology as a means of processing casework more rapidly, new sieving polymers need to be evaluated for utilization in this platform. The research presented here describes the assessment of commercially-available polymeric sieving matrices for STR analysis, with consideration given to feasibility of incorporation into a microdevice. Polymer composition, molecular weight, and concentration were evaluated, along with an assessment of the effects of buffer composition, separation temperature, and capillary length. These variables were evaluated individually or collectively on the ability to resolve STR DNA fragments and the reproducibility of the separations and the results compared to a proprietary commercial product. A 600,000 Da MW poly(ethylene oxide) (PEO) solution at a 3% (w/v) concentration was determined to be the most suitable matrix for these separations. This polymer, in coated capillaries, provided highly robust and reproducible separations, with near baseline resolution of fragments having single base differences. Reductions in the temperature of the separation, from 60 degrees C to 40 degrees C, and the urea concentration of the buffer, from 7 M to 3.5 M, provided increased longevity of the PEO polymer for repeated separations. Comparison of this polymer with currently specified commercial products used for STR analysis showed that the optimized PEO matrix provided superior separations under all conditions tested. In addition, PEO could be utilized in shorter capillary systems, with a concurrent decrease in analysis time, highlighting its potential for use in shortened capillary or microdevice systems. 相似文献
12.
The influence of crime among the elderly was investigated in this study. The study suggests that crime prevention programs among the elderly should be implemented with some reservations. Since the risks of criminal victimization are low among the elderly, it may not be possible to reduce those rates much further, even with elaborate security programs for the elderly. On the contrary, raising the salience of crime by placing great emphasis on preventive behavior may well foster social isolation among the elderly. 相似文献
13.
The article describes a study of the perceptions of three groups--patients, orthopaedic surgeons and the surgeons' practice managers--concerning three types of legal risk associated with the duty of care: failure to follow up, failure to warn and failure to diagnose. The study found there is cause for concern about doctors' follow-up and documentation of patient care. Doctors may be unaware of the Australian courts' propensity to emphasise practitioner responsibility rather than patient autonomy. A further important result is the considerable disparity between the surgeons' views and the views of their practice managers about the duty of care. The article draws out implications for improved risk awareness and suggests further research. 相似文献
14.
Jerome Kagan 《Journal of youth and adolescence》1976,5(2):113-129
Recent empirical findings viewed from a cognitive developmental perspective suggest new interpretive stances toward three familiar themes —the fears of infancy, the discontinuous quality of stages in cognitive functioning, and the capacity for resilience in cognitive development. (1) Time of onset and decline of separation anxiety in infants are primarily a function of the infant's level of cognitive development rather than variation in interactive experiences with the caretaker. (2) Cognitive competences seem to be severely limited to specific problem contexts, and we should not talk of competences in the abstract. Hence stages should be viewed as continuous and gradual. (3) Young mammals seem to retain a capacity for recovery from early experiences that retard normal development if they are fortunate enough to be moved to a more benign context.The research summarized in this article was supported over the past 18 years by grants from the National Institute of Child Health and Human Development, the National Institute of Mental Health, the National Science Foundation, the Carnegie Corporation of New York, the Spencer Foundation, the Office of Child Development, the Foundation for Child Development, and the Grant Foundation.Delivered at the Tenth Anniversary of Pritzker Children's Psychiatric Unit of Michael Reese Hospital and Medical Center, Dr. P. Devryer, Director, Chicago, Illinois 60615, March 8, 1975.Received his Ph.D. in psychology from Yale University in 1954. Joint concentration in both physiological and personality development. Current interests are in the growth of cognitive, social, and affective systems during the first decade of life. 相似文献
15.
Jerome T. Barrett 《Negotiation Journal》1999,15(3):219-228
Although people have their choice today of an ever-growing number of how-to books and articles about the mediation process, this plenitude was hardly always the case. In fact, back in the early 1950s in the United States, three young mediators with the Federal Mediation and Conciliation Service (FMCS) drafted a 62-page memo on the subject of their work which included 120 different techniques of mediation, many of which would be familiar and useful to practitioners today. Their pioneering work, however, was neither recognized nor encouraged by the FMCS; in fact, it was suppressed. This article focuses on the reasons for the suppression of that work, providing an overview of the vast changes that have taken place in mediation's history and the federal labor-management mediation agency over the past half-century. 相似文献
16.
Jerome Neu 《Criminal justice ethics》2013,32(2):62-67
Norval Morris, Madness and the Criminal Law Chicago: The University of Chicago Press, 1982, 235 pp. 相似文献
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