Quantification of psilocin in human whole blood using liquid chromatography–tandem mass spectrometry (LC–MS/MS)

There has been burgeoning interest in psilocybin-use for the treatment of various neurological and neurodegenerative diseases. Psilocybin is mistakenly perceived as the principal pharmacologically active compound due to its high concentrations found in magic mushrooms; however, it is the prodrug of psilocin. Despite the expanding body of clinical research seeking to understand the pharmacodynamic/pharmacokinetic properties of psilocin, and its role in inducing dramatic changes to cognitive function, there has not been a corresponding increase in the development of sensitive analytical methods that can quantify psilocin in different biological fluids. Existing analytical methods have been developed using plasma, serum, and urine as the matrix of choice, but with the unknown blood-to-plasma ratio of psilocin, any pharmacokinetic conclusions drawn solely on plasma data may be misleading. Thus, the main objective of this study is to develop the first analytical method that utilizes SPE and LC–MS/MS to quantify psilocin in human whole blood. The SPE procedure yielded a high recovery efficiency (≥89%) with minimal matrix effects. The method was validated according to ANSI/ASB 036 guidelines. Linearity was between 0.7–200 ng/mL and encompassed previously reported ranges found in plasma/serum. Bias, within- and between-run precision for all quality controls met ANSI/ASB 036 acceptability criteria. Endogenous/exogenous interferences and carryover were negligible. Psilocin stability was assessed at 4°C over 48 h and was considered stable. Although a proof-of-concept study will need to be performed to characterize the method, this analytical workflow was able to detect and quantify psilocin in human whole blood at low limits of quantification.     

Investigating Residential History Using Stable Hydrogen and Oxygen Isotopes of Human Hair and Drinking Water

The relationship between isotopic signals in human hair and geographic region has potential forensic applications for identifying unknown individuals' place of recent residence. This study analyzes δ²H and δ¹⁸O isotopes in residential tap water and bulk hair samples from 17 volunteers representing 12 locations in Ontario, Canada. There is a strong correlation (R² = 0.9) between δ²H and δ¹⁸O values of the water samples. In contrast, the δ²H and δ¹⁸O values of the hair samples are weakly correlated (R² = 0.3), and the greater variability in the data is linked to dietary factors. This study demonstrates that the δ²H and δ¹⁸O values of hair and drinking water can be used to help identify potential place of residence in forensic cases, particularly in relation to proximity to large bodies of water such as the Great Lakes, but interpretations are complicated by the contribution of both water and diet to δ²H and δ¹⁸O values in hair.     

Introduction: New directions in agrarian political economy

For four decades, The Journal of Peasant Studies (JPS) has served as a principal arena for the formation and dissemination of cutting-edge research and theory. It is globally renowned as a key site for documenting and analyzing variegated trajectories of agrarian change across space and time. Over the years, authors have taken new angles as they reinvigorated classic questions and debates about agrarian transition, resource access and rural livelihoods. This introductory essay highlights the four classic themes represented in Volume 1 of the JPS anniversary collection: land and resource dispossession, the financialization of food and agriculture, vulnerability and marginalization, and the blurring of the rural-urban relations through hybrid livelihoods. Contributors show both how new iterations of long-evident processes continue to catch peasants and smallholders in the crosshairs of crises and how many manage to face these challenges, developing new sources and sites of livelihood production.     

Retelling the past,inspiring the future: Waffen-SS commemorations and the creation of a ‘European’ far-right counter-narrative

ABSTRACT

Heinrich Himmler created the Waffen-SS in part as a multinational force, willing to fight for a New Europe based on Germanic blood. After the war, many international Waffen-SS units formed veterans' associations (VAs). Like other VAs, these provided veterans with the chance to engage in ‘memory work’ and to keep alive a sense of comradeship and of valiant sacrifice, as well as an emotional commitment to the fallen. Waffen-SS veterans were, however, alone in celebrating their ‘sacrifices’. Others shunned them for their participation in atrocities. To defend themselves, they developed a counter-hegemonic Second World War narrative that presented the Waffen-SS as uniquely heroic ‘European’ volunteers' against Bolshevism. This counter-narrative, however, only gained resonance with the fall of the Berlin Wall. After 1989, in fact, veterans could seek out and establish sites of public commemoration, not in Western but in Eastern Europe. Hurd and Werther use veterans' journals and books to explore the redeployment of SS ideology in a revisionist version of history. They examine the resurrection of a mass Waffen-SS graveyard in East Ukraine as a telling case history, discussing, not least, the implications of a ‘reconciliation’ of the former German soldiers with both Ukrainian villagers and Red Army veterans. Finally, they explore the significance of the veterans' ‘European’ counter-history for a younger generation of neo-Nazis.