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151.
Krenke BE Viculis L Richard ML Prinz M Milne SC Ladd C Gross AM Gornall T Frappier JR Eisenberg AJ Barna C Aranda XG Adamowicz MS Budowle B 《Forensic science international》2005,148(1):1-14
Y chromosome-specific short tandem repeat (Y-STR) analysis has become another widely accepted tool for human identification. The PowerPlex Y System is a fluorescent multiplex that includes the 12 loci: DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. This panel of markers incorporates the 9-locus European minimal haplotype (EMH) loci recommended by the International Y-STR User Group and the 11-locus set recommended by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Described here are inter-laboratory results from 17 developmental validation studies of the PowerPlex Y System and include the following results: (a) samples distributed between laboratories and commercial standards produced expected and reproducible haplotypes; (b) use of common amplification and detection instruments were successfully demonstrated; (c) full profiles were obtained with standard 30 and 32 cycle amplification protocols and cycle number (24-28 cycles) could be modified to match different substrates (such as direct amplification of FTA paper); (d) complete profiles were observed with reaction volumes from 6.25 to 50 microL; (e) minimal impact was observed with variation of enzyme concentration; (f) full haplotypes were observed with 0.5-2x primer concentrations; however, relative yield between loci varied with concentration; (g) reduction of magnesium to 1mM (1.5 mM standard) resulted in minimal amplification, while only partial loss of yield was observed with 1.25 mM magnesium; (h) decreasing the annealing temperature by 2-4 degrees C did not generate artifacts or locus dropout and most laboratories observed full amplification with the annealing temperature increased by 2 degrees C and significant locus dropout with a 4 degrees C increase in annealing temperature; (i) amplification of individual loci with primers used in the multiplex produced the same alleles as observed with the multiplex amplification; (j) all laboratories observed full amplification with >or = 125 pg of male template with partial and/or complete profiles observed using 30-62.5 pg of DNA; (k) analysis of < or = 500 ng of female DNA did not yield amplification products; (l) the minor male component of a male/female mixture was observed with < or =1200-fold excess female DNA with the majority of alleles still observed with 10,000-fold excess female; (m) male/male mixtures produced full profiles from the minor contributor with 10-20-fold excess of the major contributor; (n) average stutter for each locus; (o) precision of sizing were determined; (p) human-specificity studies displayed amplification products only with some primate samples; and (q) reanalysis of 102 non-probative casework samples from 65 cases produced results consistent with original findings and in some instances additional identification of a minor male contributor to a male/female mixture was obtained. In general, the PowerPlex Y System was shown to have the sensitivity, specificity and reliability required for forensic DNA analysis. 相似文献
152.
Validation of male-specific, 12-locus fluorescent short tandem repeat (STR) multiplex 总被引:1,自引:0,他引:1
Krenke BE Viculis L Richard ML Prinz M Milne SC Ladd C Gross AM Gornall T Frappier JR Eisenberg AJ Barna C Aranda XG Adamowicz MS Budowle B 《Forensic science international》2005,151(1):111-124
Y chromosome-specific short tandem repeat (Y-STR) analysis has become another widely accepted tool for human identification. The PowerPlex Y System is a fluorescent multiplex that includes the 12 loci: DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. This panel of markers incorporates the 9-locus European minimal haplotype (EMH) loci recommended by the International Y-STR User Group and the 11-locus set recommended by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Described here are inter-laboratory results from 17 developmental validation studies of the PowerPlex Y System and include the following results: (a) samples distributed between laboratories and commercial standards produced expected and reproducible haplotypes; (b) use of common amplification and detection instruments were successfully demonstrated; (c) full profiles were obtained with standard 30 and 32 cycle amplification protocols and cycle number (24-28 cycles) could be modified to match different substrates (such as direct amplification of FTA paper); (d) complete profiles were observed with reaction volumes from 6.25 to 50 microL; (e) minimal impact was observed with variation of enzyme concentration; (f) full haplotypes were observed with 0.5-2x primer concentrations; however, relative yield between loci varied with concentration; (g) reduction of magnesium to 1mM (1.5 mM standard) resulted in minimal amplification, while only partial loss of yield was observed with 1.25 mM magnesium; (h) decreasing the annealing temperature by 2-4 degrees C did not generate artifacts or locus dropout and most laboratories observed full amplification with the annealing temperature increased by 2 degrees C and significant locus dropout with a 4 degrees C increase in annealing temperature; (i) amplification of individual loci with primers used in the multiplex produced the same alleles as observed with the multiplex amplification; (j) all laboratories observed full amplification with >or = 125 pg of male template with partial and/or complete profiles observed using 30-62.5 pg of DNA; (k) analysis of < or = 500 ng of female DNA did not yield amplification products; (l) the minor male component of a male/female mixture was observed with < or =1200-fold excess female DNA with the majority of alleles still observed with 10,000-fold excess female; (m) male/male mixtures produced full profiles from the minor contributor with 10-20-fold excess of the major contributor; (n) average stutter for each locus; (o) precision of sizing were determined; (p) human-specificity studies displayed amplification products only with some primate samples; and (q) reanalysis of 102 non-probative casework samples from 65 cases produced results consistent with original findings and in some instances additional identification of a minor male contributor to a male/female mixture was obtained. In general, the PowerPlex Y System was shown to have the sensitivity, specificity and reliability required for forensic DNA analysis. 相似文献
153.
Gender Patterns of Research and Licensing Activity of Science and Engineering Faculty 总被引:1,自引:0,他引:1
Jerry?G.?ThursbyEmail author Marie?C.?Thursby 《The Journal of Technology Transfer》2005,30(4):343-353
In this paper we extend our earlier work on science and engineering faculty disclosure and licensing activity to examine a characteristic of faculty ignored in our earlier work – the gender of faculty in our database of over 4500 faculty at 11 major universities. Not surprisingly, women comprise only 8.55% of the faculty in our sample. They are most represented in the biological sciences and the women in the sample are more likely to be younger faculty. The most striking result from highlighting women in the sample is that women are less likely to disclose inventions than men despite the fact that there are no significant differences in publication patterns. While disclosure activity varies by gender, we find that the disclosure activity of women and men converges over the period of our sample.JEL Classification: J16, L3, O3Financial support was provided by National Science Foundation (SES 0094573), the Alan and Mildred Peterson Foundation and the Ewing Marion Kauffman Foundation. 相似文献
154.
155.
Valrie Martin Marie‐Hlne Jobin 《Canadian public administration. Administration publique du Canada》2004,47(3):304-331
Au cours des deux derniéres décennies, plusieurs gouvemements ont voulu réformer leur administration publique en mettant en place un cadre de gestion axée sur les résultats. Jusqu à tout récemment, la documentation scientifique n'abor‐dait que trés peu ce théme et, outre les travaux de l'OCDE, le concept de gestion axée sur les résultats et son contenu n' avaient pas été définis. Nous avons donc voulu éla‐borer une définition générique de la gestion axée sur les résultats et comprendre la façon dont elle s'intègre à différents aspects de la gestion des organisations. Pour ce faire, nous avons exploré le cadre de gestion proposé par les réformes mises de l'avant dans huit juridictions. Les pays et provinces canadiennes choisis ont une approche‐type de gestion anglo‐américaine, soit le Canada, le Québec, l'Ontario, l'Alberta, les États‐Unis, l'Australie, le Royaume‐Uni et la Nouvelle‐Zélande. Les modèles étudiés présentent plusieurs similarités. On observe que la gestion de la performance dans ces juridictions se déploie dans des cycles de gestion composés de plusieurs étapes. Les éléments communs de ces cycles sont la planification stratégique, la planification opérationnelle, la réalisation des plans et des programmes, la mesure ds résultats, la reddition de comptes et l'analyse et l'interpréta‐tion des résultats. Ces processus se concrétisent par la publication de documents. Par ailleurs, ces cadres de gestion ont tendance à s'intégrer aux autres enjeux de la gestion des organisations tels que la qualité du service à la clientèle, la gestion des res‐sources humaines et financières et la vérification. Abstract: Over the last two decades, several governments have wanted to reform their public administration by implementing a results‐based management framework. Until very recently, the scientific literature hardly addressed this issue and, other than the OECD work, the concept of results‐based management and its content had not been defined. We therefore wanted to develop a generic definition for results‐based management and to understand how it fits into various aspects of organizational management. To do so, we explored the management framework put forward through reforms introduced in eight jurisdictions. The countries and Canadian provinces chosen, i.e., Canada, Quebec, Ontario, Alberta, United States, Australia, United Kingdom and New‐Zealand, use the typical Anglo‐American management approach. The models considered have several similarities. We found that performance management in those jurisdictions was implemented throughout several steps of management cycles. Common elements in these cycles include strategic planning, operational planning, plan and program delivery, results measurement, accountability, and results analysis and interpretation. The process is documented through published documents. Moreover, these management frameworks tend to become part of the other organizational management components, such as quality of customer service, management of human and financial resources, and auditing. 相似文献
156.
This article, to be published in two parts, explores the debate between the Bertelsmann Foundation and the Centre for Democracy and Technology about regulation on illegal and harmful content on the Internet. The authors intend to analyze the method, interests and limits of the self regulatory model proposed by the European institutions, bearing in mind the users’ empowerment perspective. 相似文献
157.
ABSTRACTWe tested the utility of applying the Verifiability Approach (VA) to witness statements after a period of delay. The delay factor is important to consider because interviewees are often not interviewed directly after witnessing an event. A total of 64 liars partook in a mock crime and then lied about it during an interview, seven days later. Truth tellers (n?=?78) partook in activities of their own choosing and told the truth about it during their interview, seven days later. All participants were split into three groups, which provided three different verbal instructions relating to the interviewer’s aim to assess the statements for the inclusion of verifiable information: no information protocol (IP) (n?=?43), the standard-IP (n?=?46) and an enhanced-IP (n?=?53). In addition to the standard VA approach of analysing verifiable details, we further examined verifiable witness information and verifiable digital information and made a distinction between verifiable details and verifiable sources. We found that truth tellers reported more verifiable digital details and sources than liars. 相似文献
158.
159.
Marie Léoutre 《议会、议员及代表》2013,33(1):22-39
SUMMARYThis paper explores the constitutional relationship between England and Ireland at the end of the seventeenth century with a focus on the contemporary debate around the prerogative of the Irish legislature. It examines and contrasts the arguments developed in the pamphlets of William Molyneux of Dublin (1656–98), representing the ruling Irish Protestant nation, and of English Whig Simon Clement (1654?–1730?), asserting the rights of the English empire. Molyneux's The Case of Ireland's being bound by Acts of Parliament in England, Stated (Dublin, 1698) and Clement's An answer to Mr. Molyneux his case of Ireland's being bound by Acts of Parliament in England, stated: and his dangerous notion of Ireland's being under no subordination to the parliamentary authority of England refuted by reasoning from his own arguments and authorities (London, 1698) are compared and analysed in the context of renewed tensions around the woollen trade. These pamphlets highlight the nature, and the perceived nature, of the constitutional relationship between the two polities in the aftermath of the ‘Glorious Revolution’ of 1688. The main discussion was whether Ireland was a colony of England or an independent kingdom, and how its nature affected the relationship between both legislatures. Molyneux argued that Ireland, although sharing a monarch with England, was an independent kingdom. To Molyneux, Ireland's independence, as a kingdom, signified the independence of its legislature and the unconstitutionality of the English Parliament's claim to legislate for Ireland. Clement refuted Molyneux's assertions point by point, contending that Ireland was part and parcel of an empire. In Clement's opinion, Ireland's subjection to England meant that the English Parliament had a legitimate right to legislate for Ireland. 相似文献
160.
ABSTRACTThe role of resources in war has been much debated. What happens when foreign patrons provide lavish amounts of cash to rebels, without mechanisms of accountability? This article analyzes three major sources of funding and their micro-level effects on insurgent-groups in the Syrian civil war. Recipients of funding demonstrated opportunism in actions, alliances, and ideologies, directly related to the funding source. Funders thus set the agenda of the war, promoting Islamist ideologies and regional over local issues. Private donors rivaled state sponsors, in what may be a harbinger of future globalization trends. 相似文献