Doping control analysis of selected peptide hormones using LC-MS(/MS)

With the constantly increasing sensitivity and robustness of liquid chromatography-mass spectrometry-based instruments combined with enhanced reproducibility as well as mass accuracy and resolution, LC-MS(/MS) has become an integral part of sports drug testing programs particularly concerning the detection of peptide hormones. Although several of the relevant peptidic drugs such as insulins (Humalog LisPro, Novolog Aspart, etc.), growth hormone releasing peptides (GHRPs, e.g., GHRP-2, GHRP-6, Hexarelin, etc.), and insulin-like growth factors (e.g., IGF-1, IGF-2, long-R(3)-IGF-1) are currently analyzed using dedicated top-down analytical procedures, i.e. employing specifically tailored sample preparation procedures followed by targeted LC-MS(/MS) measurements focusing on intact analytes, first approaches towards multi-analyte methods have been established. These allow the determination of the prohibited substances in blood and urine doping control specimens following therapeutic applications. In addition, the use of new complementary devices such as ion mobility analyzers, e.g., in hybrid mass spectrometers yielded promising data for the differentiation of isobaric insulins, which outlines the potential to further accelerate and multiplex doping control analytical assays to meet the continuously increasing demands of rapid and unambiguous test methods. Moreover, the potential of LC-MS/MS to target recombinant peptide hormones such as human growth hormone using bottom-up approaches has been demonstrated by targeting proteotypic peptides that unambiguously differentiate the recombinant molecule from the naturally occurring and endogenously produced analog.     

Reverse engineering--rapid prototyping of the skull in forensic trauma analysis

Rapid prototyping (RP) comprises a variety of automated manufacturing techniques such as selective laser sintering (SLS), stereolithography, and three-dimensional printing (3DP), which use virtual 3D data sets to fabricate solid forms in a layer-by-layer technique. Despite a growing demand for (virtual) reconstruction models in daily forensic casework, maceration of the skull is frequently assigned to ensure haptic evidence presentation in the courtroom. Owing to the progress in the field of forensic radiology, 3D data sets of relevant cases are usually available to the forensic expert. Here, we present a first application of RP in forensic medicine using computed tomography scans for the fabrication of an SLS skull model in a case of fatal hammer impacts to the head. The report is intended to show that this method fully respects the dignity of the deceased and is consistent with medical ethics but nevertheless provides an excellent 3D impression of anatomical structures and injuries.     

The Non-Procedural Determinants of Responsiveness

This article starts from the remarks by Peter Mair on the growing gap between responsiveness and responsibility – or middle-run responsiveness – and the declining capacity of parties to bridge that gap. It focuses on the empirical analysis of the association between economic and substantive democratic dimensions and responsiveness, which are highly relevant to the way in which parties compete and govern within contemporary democracies. Following an introduction of the topic, the second section puts forward key concepts and hypotheses; the third presents the operationalisation of the variables and the applied method; the fourth and primary empirical section of the article analyses the non-procedural determinants of political and economic responsiveness, including freedom and equality as well as several key economic structural factors. The concluding remarks recapitulate the main empirical findings and submit a number of aspects that party leaders ought to take into account when addressing the thorny issue of responsiveness.     

Morphology of low-velocity impact stains produced from single drops of blood

Systematic variation of blood droplet volume, the distance fallen and the surface (paper, wood, plastics, tiles) led to the conclusion that the size and the shape of the stains ("fingers", satellites) allowed to deduce the distance fallen but only if the actual surface structure was known. We found that detailed photography at the crime scene was necessary, yet experiments have to be performed due to the extreme influence of the actual surface texture on all characteristics (size, spines, peripheral spatter) of the blood stains.     

Camouflaging Facial Emphysema: A new syndrome

Some cosmetic products manufactured in Korea for the treatment of eczema, seborrhea and psoriasis have been suspected to contain anti-inflammatory corticosteroids such as prednisolone, hydrocortisone, betamethasone, dexamethasone and triamcinolone acetonide without these ingredients being indicated on the label. Due to their severe side effects such as permenent skin atopy, these corticosteroids have to be monitored in cosmetic products from a forensic point of view. Many cosmetic product samples (N=65) have been collected from both local and online markets in Korea. The corticosteroid content of these samples was analyzed by LC-MS/MS with diagnostic ions (m/z). Linearity was studied with 0.1-10 μg/mL range in all corticosteroids. Good correlation coefficients (r(2)≥0.997) were found and the limits of quantification were 4.68-7.97 ng/mL for each of the corticosteroids. At three different concentrations spanning the linear dynamic ranges, mean recoveries were 97.2-113.5%and precisions (RSD) for intra-day and inter-day analysis were less than 8.9%. Also, accuracy (Bias %) was less than 11.8%. The results showed that between 0.76-0.94 μg/g levels of prednisolone were detected in four cosmetic products and triamcinolone acetonidewas detected with a concentration in the range of 11.5-272 μg/g in nine samples. This fact reveals that some manufacturers have arbitrarily added these corticosteroids in their cosmetic products without indicating them on the label. Thus, these cosmetic products have to be monitored and if proven illegal preparations removed from the market.     

Current trends in nanotechnology research across worldwide geo-economic players

The purpose of this paper is to analyze the current temporal and spatial trajectories in nanotechnology research in order to display the worldwide patterns of scientific domains across main geo-economic players. Current trends of nanotechnology have been growing in chemistry and medicine because of technological applications of new nanomaterials mainly in Chemical Engineering, Biochemistry, Genetics and Molecular Biology. In addition, the results show that the laboratories of Europe and North America have intensive scientific collaborations with foreign scholars in nanotechnology studies. This paper can provide vital findings to support new research and innovation policies in order to foster the development of these driving converging technologies for future patterns of economic growth.     

Laudatio: Verleihung des Theodor Eschenburg-Preises an Prof. Dr. Gerhard Lehmbruch am 25. September 2003 auf dem Kongress der Deutschen Vereinigung für Politische Wissenschaft in Mainz

Ohne Zusammenfassung     

Short Term Change in Attitude and Motivating Factors to Change Abusive Behavior of Male Batterers after Participating in a Group Intervention Program Based on the Pro-Feminist and Cognitive-Behavioral Approach

The Domestic Abuse Education Project (DAEP), in Burlington, Middlebury, and St. Albans, Vermont, is a group based domestic abuse intervention program, based in a pro-feminist and cognitive-behavioral approach for domestic violence intervention and prevention. A pre and post-test instrument was developed and implemented to determine short-term change in attitude of participants and motivating factors to change behavior, after completing the twenty-seven session program. After the program, participants reported a positive change in attitudes regarding their abusive behavior and stereotypical beliefs about women. Participants were also more motivated to change their behavior by the effect abuse has on their family relationships. However, many participants continued to agree that insecurity, jealousy, and alcohol and drug use can cause violence. The positive changes in attitude and motivational factors show that this is an effective model in changing underlying batterer attitudes that provide rationale for abusive behavior.     

The Psychology of Political Extremism

Any attempt to analyse the phenomenon of political extremism must seek to explain why choices are sometimes made to articulate such views through violence. It is proposed that such a choice may have little to do with the specific ideology through which extremism is expressed but instead may reflect deeper psychological considerations. Investigations of persons who grew up in totalitarian systems suggest that the state may invade the psyche, interfering with the normal interplay of ego, superego and id. An explanation depending on the construction of a tripartite model of personal, non-personal and impersonal self was developed to explain the consequences of this process. It is proposed that such models may have a wider relevance and can be applied to the phenomenon of violence arising from contemporary political extremism. The advantage of such models is that they can be subjected to empirical investigation.