Reduced volume PCR amplification reactions using the AmpFlSTR Profiler Plus kit

The forensic community continues to seek improvements in DNA typing methods on aspects such as sensitivity and efficacy. Reducing the volume of the AmpFlSTR Profiler Plus reagents offered greater sensitivity and improved the chance of obtaining useful results for samples with very low quantities of DNA and multiple source samples. On the downside, amplifications initiated with less than 0.4 ng of DNA exhibited a twofold increase in the standard deviation of peak ratios. This research suggested a twofold approach to analyzing samples. For samples with greater than 0.25 ng of DNA, a 25 microL reaction is appropriate. Samples that did not demonstrate quantifiable results, or that have less than 0.25 ng, can be amplified by drying the sample directly in the PCR tube and amplifying in a 5 microL reaction. The analyst can expect at least limited results with as little as 0.03 ng of DNA in the 5 microL reaction.     

Identification of anhydroecgonine ethyl ester in the urine of a drug overdose victim

Toxicological evaluation of postmortem urine collected from a 41-year-old deceased white male detected anhydroecgonine ethyl ester (ethylecgonidine, AEEE), a transesterification product of smoked cocaine co-abused with ethanol. A solid phase extraction (SPE) method was used to extract cocaine, AEEE, and related metabolites from urine. SPE on a 1 mL urine sample from the decedent followed by GC-MS detected AEEE. Other metabolites identified by GC-MS included cocaine, cocaethylene, and anhydroecgonine methyl ester (AEME). To determine whether some or all of the AEEE was artifactually produced in the heated GC injector port, an alternative LC-MS method was developed. LC/MS following SPE found at least 50 ng/mL of AEEE in the extract. The mass fragmentation (MS/MS and MS3) of AEEE detected in the urine was compared to spectra of authentic, synthesized compound. AEEE is a potential additional forensic marker for the co-abuse of smoked cocaine and ethanol.     

Romantic Relationships and Body Satisfaction Among Young Women

In the present study we examined associations between young women's romantic relationships and their body image. Ninety-five heterosexual couples (women's mean age=22.46 and men's mean age=24.38) participated in this study. We examined young women's satisfaction with their own bodies, their perceptions of their significant others’ satisfaction with their bodies, and their significant others’ actual satisfaction with their bodies using the Contour Drawing Rating Scale women's weight status was assessed using body mass index (BMI). Relationship constructs examined included relationship quality and the length of couples’ romantic relationships. Results indicated that females were more dissatisfied with their bodies than they perceived their significant others to be and were more dissatisfied than their significant others actually were. Analyses further indicated that the longer women had been in a relationship with their significant other, the more likely they were to incorrectly believe that their significant other wanted them to look thinner.Assistant Professor at Rutgers University. She received her PhD in psychology from the University of California at Riverside in 2002. Her research focuses on the development of body image and dieting behaviors and on understanding relations between personality and health.Assistant Professor at Villanova University. He received his PhD in psychology from the University of California at Riverside in 2002. His research focuses primarily on interpersonal theory.     

Reconstruction of ripped-up documents using fragment stack analysis procedures

Reconstruction of ripped-up documents can be a very time-consuming task for forensic experts. Currently, this task is often carried out using various homespun or loosely defined procedures. Under the main assumption that a (partially) ordered set of fragments can be recovered, we propose and discuss a more formal analysis methodology for this type of reconstruction problem. We discuss several complications that can occur in real-life problems and illustrate the efficiency of the proposed methods. Although several avenues for further research remain, we show that the proposed approach offers both a better understanding of the problem, as well as important strategies for devising very fast manual and semi-automatic fragment reassembly procedures.     

Capacity builders for governance: community-driven reconstruction in the eastern Democratic Republic of Congo

Community-driven reconstruction (CDR) has become a new paradigm in post-conflict development. It combines infrastructure restoration with introducing good governance at the local level. Recent evaluations show that governance objectives are not easily met and significant change cannot be demonstrated. This paper adds to this argument on the basis of ethnographic research on a CDR programme in eastern DRC. It seeks to find explanations for the lack of demonstrable governance impact in the content and implementation of training. It identifies room for improvement by better adjusting capacity building to locally prevailing accountability mechanisms and by coordinating capacity building with other development programmes.     

Local government,multilevel governance,and cluster‐based innovation policy: Economic cluster strategies in Canada’s city regions

This article examines the relationship between local government economic development policy and multilevel governance, and investigates whether multilevel governance can be effectively implemented in key sectors, using two case studies of the aerospace and fashion sectors. This article seeks to answer the following questions: What inhibits the formation of multilevel collaborative governance institutions in these sectors? What implications does this have for urban economic development policy for the City of Toronto, and by extension, other cities across Canada? The case study evidence suggests that these institutions are forming in other cities such as Montreal and contributing to their success. So why is Toronto different?     

Retract,Expand: Libel Law,the Professionalization of Journalism,and the Limits of Press Freedom at the Turn of the Twentieth Century

At the turn of the twentieth century, many judges and juries considered libel law to be a robust check against a reckless and sensational press. So how did the newspaper industry convince seventeen state legislatures between 1885 and 1915 to pass laws easing liability for accidental libel? This article analyzes a debate surrounding libel law reform to tell the story of how retraction statutes were conceived in press association meetings, enacted in spite of lawyers' reservations, and challenged in state supreme courts during a pivotal era in the professionalization of journalism. It offers a better understanding of the power of the institutional press to influence Americans' conception of the purpose of press freedom and the role of journalism in democratic society, and a clearer picture of the beginnings of a more press friendly era in libel law, from the wire service defense to the actual malice standard.