Lifestyle illicit drug seizures: A routine ESI-LC-MS method for the identification of sildenafil and vardenafil

For street samples suspected of containing the phosphodiesterase-5 inhibitors sildenafil (Viagra(?)) and/or vardenafil (Levitra(?)), including powders or adulterated herbal supplements, a chemical analysis is needed to provide confirmatory identification of these illegally procured substances. Sildenafil and vardenafil are structurally similar and it is difficult to differentiate between them, as previous mass spectrometric studies have shown the two drugs to produce similar fragmentation patterns. The use of tandem mass spectrometry can produce confirmatory data, but the technique requires a high level of technical expertise. We have developed an electrospray ionization-liquid chromatography-mass spectrometry (ESI-LC-MS) method that allows differentiation between these two structurally similar molecules via in-source fragmentation in combination with an ion trap mass spectrometer. A very stable gas phase ion is formed during in-source fragmentation of vardenafil; the combination of the stability of this ion and the longer residence time for the ion in the ion trap results in a very strong signal. This feature results in a method that can provide clear differentiation between sildenafil and vardenafil while at the same time requiring less expertise from the routine analyst to confirm the presence or absence of the two compounds.     

Validation of reduced-scale reactions for the Quantifiler Human DNA kit

Accurate quantification of DNA samples is an important step in obtaining accurate and reproducible short tandem repeat (STR) profiles. Quantitative real-time-PCR has improved the speed and accuracy of DNA quantification over earlier methods, albeit at significantly greater cost per reaction. Here, the performance of reduced volume (10 microL) DNA quantification assays using the Quantifiler Human DNA Quantification Kit was evaluated using commercial standards and single source biological stains (e.g., venous blood, saliva, and semen). In addition, casework-type samples including those subjected to environmental contaminants containing PCR inhibitors and samples having undergone extensive DNA degradation were also quantified. The concentration of DNA in various forensic samples ranged from 0 to 2.9 ng/microL depending on sample source and/or environmental insult. Compared to full-scale reactions, reduced volume assays displayed equivalent to improved amplification efficiency and sample-to-sample reproducibility (+/-0.01-0.17 C(T FAM)). Furthermore, the use of data from reduced-scale Quantifiler reactions facilitated the accurate determination of the amount of sample DNA extract needed to generate quality STR profiles. The use of 10 microL-scale Quantifiler reaction volumes has the practical benefit of increasing the effective number of reactions per kit by 250%; thereby reducing the cost per assay by 60% while consuming less sample. This is particularly advantageous in cases of consumptive testing.     

Civilising the police: reconceptualising the role of the state in theories of American policing

The three eras in American policing – political, reform, and community – has become the default theoretical framework within the study of criminal justice, explicitly and implicitly shaping the discourse of police studies. Despite historically informed criticisms of this three-era model, no alternative theory has been proffered as a way of critically thinking about the police. This paper draws on Norbert Elias’ civilising thesis and the role of the state as an alternative theoretical framework to explain the evolution of American policing. We argue that changes in policing are more cogently explained by assuming a long-term view of change and that the intrusion and the retreat of the state from society better captures the evolution of the police through time.     

"A Very Present Menace"? Attlee, Communism and the Cold War!

With the end of the Cold War and the further opening of archives, the role of Western communist parties and their relationship with the former Soviet Union has been the subject of fresh scrutiny. This article examines the conviction of the British Labour Government and its security services that the Communist Party of Great Britain represented, at least in the early Cold War period, a "very present menace". The article discusses the policies of the Soviet Union in Europe and the Communist Party in Britain and explores how these shaped the perspectives of the Attlee Government, especially during the London dock strike of 1949. When placed against this background, Attlee's anti-communism can no longer be accepted, as most commentators do, as simply a product of Cold War paranoia.     

All the best things come to those who wait

‘A book may be good for nothing; or there may be onlyone thing in it worth knowing; are we to read it all through?’(Samuel Johnson) This section is dedicated to the review of ideas, articles,books, films and other media. It will include replies (and rejoinders)to articles, the evaluation of new ideas or proposals, and reviewsof books and articles both directly and indirectly related tointellectual property law. In 1966, Professor Benjamin Kaplan gave the Carpentier Lecturesat Columbia University. His subject was copyright law and eachof his three lectures examined a different aspect of that subject.The first looked at the ‘First Three Hundred and FiftyYears’, the second at ‘Plagiarism Reexamined’,and the final at ‘Proposals and Prospects’. Theselectures were     

Entrepreneurship from the Ivory Tower: Do Incentive Systems Matter?

There has been a paucity of research to date that has explored whether incentive systems—in the form of monetary payments to inventors, their department or institution, or to university technology transfer office (UTTO) personnel—affect entrepreneurial activities at U.S. universities. To shed light on whether financial incentives to scientists, their departments, and UTTO personnel effect entrepreneurial activity, we used both qualitative data (structured interviews with 128 UTTO directors) and quantitative data from surveys and databases available on the web. Our results show surprisingly and opposite to our theoretical predictions that incentives to scientists and to their departments are negatively related to entrepreneurial activity. In addition and consistent with theory-based predictions, pay to UTTO personnel is positively related to entrepreneurial activity. We conclude with a discussion that offers some implications to research, practice, and theory in the field of technology transfer.     

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