青壮年猝死综合征SCN2B、SCN4B基因检测

目的寻找SCN2B、SCN4B基因的变异位点,探讨其与青壮年不明原因夜间睡眠中猝死(SMDS)的关系。方法提取SMDS病例组及健康对照组的基因组DNA,采用聚合酶链式反应(PCR)方法扩增SCN2B、SCN4B基因编码区外显子、外显子-内含子交界区以及3'侧翼区序列,直接行DNA测序以明确遗传变异类型。结果在病例组中共检测到4个变异位点,c.237+27AG,c.*38CT,c.174CT(p.C58C)和c.*7CT。结论本研究首次对中国人SMDS病例进行了SCN2B、SCN4B基因的检测,上述基因是否为中国人SMDS的易感基因尚有待进一步研究证实。     

名誉侵权中的公共利益目的抗辩——新闻报道、舆论监督等行为的特殊免责事由

《民法典》第1025条规定了针对新闻报道、舆论监督等行为的公共利益目的抗辩,首次将新闻报道、舆论监督等行为的特殊免责事由写入法律。它需要弥补此前实务中引入的公众人物理论的不足,提供确定性规则。依据宪法,名誉权与表达自由均未被赋予绝对优先的地位,公共利益目的抗辩需要平衡好两者的关系。在适用时,可通过价值填充与规则再造实现法规范的进一步具体化。前者指根据法规范的目的及内容完成价值填充,后者指限缩案件范围,区分公共利益的界定主体与确认主体,将公共利益目的抗辩融入我国侵权责任法体系中,在个案中审慎开展利益衡量。     

马来西亚中小企业的发展路径与政策调整——一个制度变迁的分析

发展中小企业是金融危机后马来西亚经济政策的一个显著的调整.本文从马来西亚中小企业发展路径人手,分析了造成马国二元性产业组织结构的制度因素.结合制度变迁理论,文章探讨了马国政府在调整中小企业政策过程中所面临的组织成本、预期收益与有效的制度设计思路,并指出由于制度变迁的路径依赖性,马来西亚中小企业的发展将会是一个渐进的过程.     

南通汉族人群17个Y-STR基因座的遗传多态性

目的分析南通汉族人群的基因表型,评测17个Y-STR基因座在南通人群中的应用价值。方法采集343名南通汉族男性无关个体的外周血样本,通过Chelex-100法提取基因组DNA,用Amp FlSTR Yfiler~(TM)试剂盒进行基因分型,并与12个汉族人群[安徽、江苏、江西、山东、上海、浙江(1)、兰州、南阳、泸州、牡丹江、山西和浙江(2)]以及9个少数民族人群(蒙古族、锡伯族、拉萨藏族、青海藏族、哈萨克族、维吾尔族、满族、台湾排湾族和土家族)进行比较。结果南通汉族群体在17个Y-STR基因座共检出327种单倍型,单倍型多样性(haplotype diversity,HD)值为0.999 7,与其他人群间的R_(st)值范围为-0.000 6~0.263 5。多维尺度图结果显示南通汉族人群与大多数汉族人群之间差异无统计学意义,但明显有别于其他少数民族人群。结论 17个Y-STR基因座在南通汉族人群中的群体多态性高,具有法医学应用价值。     

Assessing the Effectiveness of NICHD Protocol Training Focused on Episodic Memory Training and Rapport-Building: a Study of Korean Police Officers

The present study examined the effectiveness of 2-day training programs utilizing the NICHD Protocol, with a particular focus on the episodic memory training and rapport-building phases. Ninety-seven police officers were allocated to one of four training conditions (protocol only, protocol and rapport-building, protocol and memory training, protocol and other interview issues), and mock interviews were conducted both before and after the training. The results showed that the proportions of invitations and directive questions asked significantly increased after training, whereas the proportions of option-posing and suggestive questions decreased. Although the different training programs changed interviewing behavior similarly, college students interviewed by police officers who had additional training in rapport-building and episodic memory training were more satisfied with their interviewers than those who were interviewed by police officers in the other conditions.     

分配正义与城乡基本公共服务均等化——基于同一性正义与差异性正义的分析

罗尔斯将平等的自由原则以及机会的公正平等原则和差别原则的结合作为实现正义的两个原则。我们根据人的同一性和差异性,将正义原则概括为同一性原则和差异性原则,认为正义是基于同一性正义和差异性正义相协同的协同正义。并以此运用于分配领域,分析和解决城乡基本公共服务均等化问题,认为同一性正义是其价值旨归,差异性正义是其必要限制和有益补充。     

The application of mitochondrial DNA cytochrome oxidase II gene for the identification of forensically important blowflies in Western China

Blowflies found on human corpses are important for the estimation of the postmortem interval and other questions of forensic relevance. Some of these species are difficult to differentiate morphologically, and therefore a molecular method was elaborated for species identification. Here, we describe a molecular method for rapid identification of these insects. Specific insect DNA fragments were amplified using the polymerase chain reaction, followed by direct DNA sequencing of the amplification products. Analysis of the cytochrome oxidase II sequences revealed abundant phylogenetically informative nucleotide substitutions that could identify blowfly species to species group. In contrast, because of the low level of sequence divergence of sister species, the data could not distinguish among taxa from the same species group, ie, the species within the Lucilia sericata and Lucilia cuprina groups. The molecular data support the existing species group separation of the taxa within the Calliphora. Because of the speed and accuracy of current nucleotide sequencing technology and the abundant apomorphic substitutions available from mtDNA sequences, this approach enables quick identification of species used for estimation of postmortem interval.     

应用Demirjian法进行法医学年龄推断的评价

目的对应用Demirjian法进行法医学年龄推断的准确度及可靠性进行初步评价。方法应用Demirjian法测定828名上海地区青少年(男279名,女549名,11～19周岁)的牙龄,比较所测牙龄和实际年龄的差异。结果在11～14岁年龄段Demirjian法测定的牙龄比实际年龄高估,在15～16岁年龄段低估,在17岁及17岁以上年龄段具有局限性。结论Demirjian法可以用于测定上海地区11～16岁年龄段青少年的牙龄,使用时需进行适当的修正。     

脑血管造影在法医学尸体检验中的应用

脑血管造影作为诊断脑血管疾病的"金标准",目前已经广泛地应用于临床医疗活动中,并积累了丰富的理论和实践资料,是趋于成熟的技术方法。由于因脑血管疾病引发的法医学鉴定案件较为多见,故该技术近年开始应用于法医学,显示出在法医学实践中独特而重要的应用价值。本文对脑血管造影术在法医学中的应用价值进行综述,内容包括辅助定位血管病变或损伤部位、明确出血性质、指导尸体解剖与脑组织病理取材等,并在此基础上展望了该技术在法医学中的应用前景。