Evaluation of the rapid analyte measurement platform (RAMP) for the detection of Bacillus anthracis at a crime scene

The aim of this study was to evaluate the accuracy and reliability of the rapid analyte measurement platform (RAMP) for presumptive identification of Bacillus anthracis spores. Test samples consisted of serial dilutions of spore preparations of several Bacillus species, including B. anthracis, which were tested, using the RAMP Anthrax test cartridge, according to the manufacturer's instructions. The fluorescence labelled antibody-antigen complexes were detected in the portable reader after 15 min following sample addition. Dilutions of common environmental and household powders were also tested to identify possible false positive results. B. anthracis spores were identified reliably in test samples containing more than 6000 spores. The test kits were highly specific, showing no cross reactivity with other Bacillus species or any environmental powders tested. The RAMP system for detection of B. anthracis spores, from environmental samples, showed consistent results under a variety of analytical conditions, enabling the trained user to provide a rapid, accurate preliminary risk assessment of a suspected bioterrorism incident.     

Sudden cardiac death due to giant cell inflammatory processes

Granulomatous inflammation of the myocardium may occur in a number of systemic disease processes including those with infectious etiologies such as fungal, mycobacterial and parasitic infections, as well as hypersensitivity reactions, and rarely autoimmune disorders. In many of these disorders, giant cells are components of the inflammatory infiltrate. Systemic granulomatous processes of unknown pathogenesis, most notably sarcoidosis, may also be associated with involvement of the myocardium. Occasionally, these disorders are associated with sudden death due to pathologic involvement of the heart. In contrast, giant cell myocarditis, also known as idiopathic myocarditis, a rare, frequently fulminant and fatal disorder of unknown etiology, is isolated to the heart and lacks systemic involvement. This disorder is most commonly diagnosed at autopsy. We present two cases in which sudden death resulted from a giant cell inflammatory process affecting the myocardium. Both individuals lacked antemortem diagnoses and collapsed at their respective places of employment. These cases compare and contrast the clinical and pathologic issues involved in the differential diagnoses of the subgroup of sudden cardiac deaths resulting from giant cell inflammatory processes that affect the myocardium, as well as the value of histologic examination and immunohistochemical studies.     

Gamma Irradiation as a Biological Decontaminant and Its Effect on Common Fingermark Detection Techniques and DNA Profiling

Abstract:  The use of disease-causing organisms and their toxins against the civilian population has defined bioterrorism and opened forensic science up to the challenges of processing contaminated evidence. This study sought to determine the use of gamma irradiation as an effective biological decontaminant and its effect on the recovery of latent fingermarks from both porous and nonporous items. Test items were contaminated with viable spores marked with latent prints and then decontaminated using a cobalt 60 gamma irradiator. Fingermark detection was the focus with standard methods including 1,2-indanedione, ninhydrin, diazafluoren-9-one, and physical developer used during this study. DNA recovery using 20% Chelex extraction and quantitative real-time polymerase chain reaction was also explored. Gamma irradiation proved effective as a bacterial decontaminant with D -values ranging from 458 to 500 Gy for nonporous items and 797–808 Gy for porous ones. The results demonstrated the successful recovery of latent marks and DNA establishing gamma irradiation as a viable decontamination option.     

Ideology and Polarization Among Women State Legislators

In early work on women in Congress, scholars consistently identified a tendency among women legislators to be more liberal roll‐call voters than male copartisans. Recent changes in Congress point to the polarization of women, where Democratic women remain more liberal than Democratic men but Republican women are no different from, or more conservative than, Republican men. We use newly available state legislative roll‐call data to determine whether women state legislators are more liberal or polarized than male copartisans. We find that while Democratic women state legislators remain consistently more liberal than male copartisans in most state chambers, Republican women legislators are growing more conservative. Thus, women state legislators are increasingly polarized in most U.S. states. Legislator replacement and increasing polarization among state legislators in office contribute to this effect. We argue that polarization among women legislators has implications for the representation of women in the states.     

Action Research at the Intersection of Structural and Family Violence in an Immigrant Latino Community: a Youth-Led Study

The current study addresses the lack of research exploring the social and emotional impact of anti-immigrant policy on Latino communities, and the intersection of anti-immigrant climates with other family stressors, like domestic violence (DV). In this paper we describe a qualitative study led by the participatory action research group La Voz Juvenile de Caminar Latino. Youth researchers conducted semi-structured interviews with Latino men, women, and youth participating in a domestic violence program. Using an inductive approach to thematic analysis, researchers identified themes that reflect how Latino families with histories of DV experience an increasingly anti-immigrant climate. All participants in this study described emotional stress, fear, and restrictions in their day-to-day life attributed to the anti-immigrant sociopolitical climate, and adults also spoke to work-related stress and economic insecurity. Both adults and children described harassment by strangers, coworkers, and/or peers. With regard to DV, women tended to describe immigration stress as exasperating family conflict, while men viewed these external stressors as discouraging DV. Distrust of police and other formal supports was a key theme underlying adult and youth perceptions of help-seeking for DV. The findings of this study suggest that anti-immigrant sentiment and policy creates undue stress for Latino families and barriers to formal help-seeking for DV. The participatory research process provided a corrective experience for youth witnesses of DV and prioritizes the voices of those most affected by immigration policies and violence.     

Coping with Electronic Data Discovery Issues

This article provides an overview of the steps a company should take if electronic discovery is requested from it during the course of litigation. Either by seeking a protective order or by inducing a motion to compel by objecting to the discovery requests, a ruling should be obtained from the court as to the parameters and limits of the requested discovery and who bears the cost of retrieving and producing the electronic data. If production is ordered by the court, the producing party must ensure through the use of formal review measures that any privileges protecting the data are not waived. While severe sanctions may be imposed on a party who destroys electronic evidence after litigation is initiated or when it is reasonably likely that litigation will ensue, a company may prevent a broad-scale, expensive production of electronic evidence by installing prior to any litigation a system of routine organization and disposal of non-essential electronic data.