An alternative for the extraction and storage of DNA from insects in forensic entomology

An important area of recent research in forensic entomology has been the use of insect DNA to provide identification of insects for fast and accurate estimation of time since death. This requires DNA to be extracted efficiently and in a state suitable for use in molecular procedures, and then stored on a long-term basis. In this study, Whatman FTA cards were tested for use with the Calliphoridae (Diptera). In particular, testing examined their ability to effectively extract DNA from specimens, and store and provide DNA template in a suitable condition for amplification using the polymerase chain reaction (PCR). The cards provided DNA that was able to be amplified from a variety of life stages, and thus appears to be of sufficient quality and quantity for use in subsequent procedures. FTA cards therefore appear suitable for use with calliphorids, and provide a new method of extraction that is simple and efficient and allows for storage and transportation without refrigeration, consequently simplifying the handling of DNA in forensic entomological cases.     

Parent-adolescent conflict and stress as a parent: Are there differences between being a mother or a father?

Parent-adolescent conflict is viewed as a common dimension of the family during the teenage years. The purpose of the present study was to determine whether mothers and fathers of young adolescents differ in their report of conflict with their adolescent, level of stress, and parenting competence. Furthermore, the role of age and gender of the young adolescents was examined. One hundred and twenty-two adolescents and their parents participated in the study. All families were intact. The families were divided into groups according to the adolescent's age and gender. Analyses of variance with three factors (adolescent's age, adolescent's gender, mothers versus fathers) were performed. Mothers and fathers did not differ on personal (depression) or marital adjustment and on perceived parenting competency. In contrast, in terms of parent-adolescent conflict, mothers reported a less positive relationship, a greater number of conflicts, and more intense discussions of conflicts with their adolescents. Gender and age of the adolescent were not important contributors to the analyses. The results suggest that, in terms of the variables examined in this study, conflict in the parent-adolescent relationship is the primary difference between mothers and fathers.     

A quantitative comparison of smokeless measurements

Measurements of the type and concentration of propellant and stabilizer additives in smokeless gunpowder are used by forensic scientists investigating the source of explosives and by military laboratories assuring the safety and efficacy of munitions. The National Institute of Standards and Technology recently assessed the state-of-the-practice of smokeless powder measurements through an international measurement comparison exercise. We here present results provided by the five participants (of 20 total) reporting quantitative as well as qualitative values for two handgun reloading powders. All five of these participants reported values for nitroglycerin (NG), ethyl centralite (EC), diphenylamine (DPA), and N-nitrosodiphenylamine (NnDPA). Several participants additionally reported the concentrations of secondary stabilizer decomposition products. The unstable NG propellant additive appears to be more susceptible to method-specific calibration biases then are the stabilizer additives. All results from one participant were strongly biased relative to those of the other four. The within-participant measurement uncertainties for all analytes were self-reported to be 1 to 5% relative; among the four concordant participants, the measurement ranges are 5 to 10% relative. There was little consistency among the participants as to what components of measurement variance were included in their uncertainty statements. A discussion of the certainties in these measurements and factors that affect the accuracy of gunpowder additive determinations is presented.     

The Effect of pH on Electrolyte Detection of Fingermarks on Cartridge Cases and Subsequent Microscopic Examination

Cartridge cases may contain deposited fingermarks when the firearm was loaded ( http://www.nij.gov/pubs-sum/225320.htm , J Forensic Sci, 53 , 2008 and 812). Cartridge cases can be individualized with microscopic examination. However, heat and friction degrades the deposited fingermark on the fired cartridge cases, if any on the surface. Also, unfired and fired cartridge cases are made of metal, which is a nonporous surface that does not retain fingermarks well ( http://www.nij.gov/pubs-sum/225320.htm ). This study tests the effects of pH level on fingermark clarity on brass fired and unfired cartridge cases and microscopic striation examination (MSE). Two trials were performed to determine the optimal pH in fingermark clarity for both types of cartridges. This was performed through immersion in six pH range solutions from dilutions of sulfuric acid and sodium hydroxide for 24 h for the purpose of enhancing the fingermarks on the metal. The use of the optimal neutral pH level is suggested because immersion of the cartridge cases in pH 1–3 and 3–5 affects MSE.     

Development of a Computer‐Assisted Forensic Radiographic Identification Method Using the Lateral Cervical and Lumbar Spine

Medical examiners and coroners (ME/C) in the United States hold statutory responsibility to identify deceased individuals who fall under their jurisdiction. The computer‐assisted decedent identification (CADI) project was designed to modify software used in diagnosis and treatment of spinal injuries into a mathematically validated tool for ME/C identification of fleshed decedents. CADI software analyzes the shapes of targeted vertebral bodies imaged in an array of standard radiographs and quantifies the likelihood that any two of the radiographs contain matching vertebral bodies. Six validation tests measured the repeatability, reliability, and sensitivity of the method, and the effects of age, sex, and number of radiographs in array composition. CADI returned a 92–100% success rate in identifying the true matching pair of vertebrae within arrays of five to 30 radiographs. Further development of CADI is expected to produce a novel identification method for use in ME/C offices that is reliable, timely, and cost‐effective.     

American murder mystery revisited: do housing voucher households cause crime?

This paper reviews the current state of the housing market, particularly in the shadow of the foreclosure crisis, the collapse of the financial system, and persistent unemployment. The authors outline the policy priorities necessary to facilitate the recovery of the housing market in general and to encourage comprehensive revitalization of the hardest hit communities.     

Ripple effects: the groundnut scheme failure and railway planning for colonial development in Tanganyika, 1947–1952

While the reasons for the failure of the groundnut scheme are well understood, its effects on colonial development in Tanganyika are not. Drawing from the voluminous paper trail that development planning leaves in its wake, this paper traces the effects of the groundnut scheme demise on a contemporaneous plan to build a railway across Tanganyika to the Northern Rhodesian copperbelt. Tensions arose among the railway planners – civil servants, politicians, and consultants from Britain, Africa, and the United States – when, midway through the planning process, the scale of the groundnut scheme collapse became public. I demonstrate how this revealing crisis prompted planners to eschew the project's production-oriented impetus and embrace a welfare-oriented conclusion. By demonstrating the interlinked nature of development projects, this paper proposes a new angle for studying the history of development in an era characterised by the rapid proliferation of projects.