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71.
A K Singh C McArdle M Ashraf K Granley U Mishra B Gordon 《Forensic science international》1990,47(1):1-15
Equine plasma and urine samples were analyzed by using a high-performance liquid chromatography (HPLC), enzyme-linked immunosorbent assay (ELISA) and particle concentration fluorescence assay (PCFIA). Although ELISA and PCFIA were rapid, simple and sensitive for the screening of furosemide, they did not give reproducible quantitative results. The HPLC method, which required relatively longer analysis time, provided simple and reproducible quantitative analysis of furosemide in plasma and urine. The performance of the three methods was compared for the quantitation of furosemide in plasma obtained from thoroughbred mares dosed intravenously with furosemide (500 micrograms/kg (n = 7) and 1.0 mg/kg (n = 5)). Although the plasma furosemide profiles determined by ELISA, PCFIA and HPLC were similar, ELISA and PCFIA methods exhibited considerable variation in values. At high furosemide concentrations, the PCFIA method gave better quantitative values than ELISA. However, at trace furosemide concentrations the PCFIA method gave false positive values which were not confirmed by HPLC or ELISA. The pharmacokinetic values obtained from the HPLC data and the pharmacokinetic values obtained previously from the gas chromatographic data were comparable. The data obtained by ELISA and PCFIA were not suitable for the pharmacokinetic calculations. 相似文献
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Reddy BM Naidu VM Madhavi VK Thangaraj K Langstieh BT Venkataramana P Kumar V Singh L 《Forensic science international》2005,149(1):81-97
Allele frequency for the 9 STR loci (D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D7S820) were estimated from a total of 1096 individuals belonging to 27 endogamous populations from the state of Andhra Pradesh, India, covering the entire gamut of socio-economic variation of the linguistic region. These loci are found to be highly polymorphic within the populations with high levels of average heterozygosity (>0.80). However, the allele frequency distributions are fairly uniform across the populations in case of these loci suggesting relatively greater homogeneity among the populations. Regrouping populations into five broad socio-economic categories further reiterated the homogenous pattern of genetic diversity. 相似文献
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Although suicidal behavior in children and adolescents is a major public health problem, large-scale research on suicide in this population is uncommon. In this study, we reviewed autopsy and field reports for all pediatric suicide cases referred to the New Mexico Office of the Medical Investigator from 1979 to 2005. The age-adjusted suicide rate was 4.8 per 100,000. Psychologic stressors and parasuicidal behavior were identified in some cases. Seventy-six percent of suicides occurred in the victim's home or yard, and 25% left a suicide note. In 26% of cases, alcohol or other drugs were detected in postmortem. Gunshot wound was the most common method overall (58%), followed by hanging (30%). Although the age-adjusted suicide rate is higher in New Mexico than nationally, the trends in the population are similar. With a solid understanding of the circumstances, it may be possible to predict, and hopefully prevent, future cases of child and adolescent death. 相似文献
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South Asian women are a focus area for organisations such as the UN, World Bank and WHO, where violence against women severely constrains policy instruments such as the Millennium Development Goals. The field researcher is often invisible in research space, which informs policy in practice. Through critical reflexivity we rupture the silence on researcher vulnerability, foregrounding researcher resilience as the ethical compass in the research space of gendered violence. Through narratives of researchers as development actors in the river of corrosiveness involved in acid violence research, we offer a typology for researcher resilience for consideration in research designs for policy development. 相似文献
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Trust is the bedrock of research, built on honesty, responsibility, accountability, and transparency. When embraced by all researchers and embedded in all aspects of research, these values help ensure that research has a positive impact on the research community, public, and policy makers, thereby enabling discoveries and innovations to enhance the well-being of humanity. 相似文献
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